Delta expression in post‐mitotic neurons identifies distinct subsets of adult‐specific lineages in Drosophila

Cornbrooks, Carson J.; Bland, Christin E.; Williams, Darren W.; Truman, James W.; Rand, Matthew D.

doi:10.1002/dneu.20308

Cited by 10 publications

(7 citation statements)

References 41 publications

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“…These results indicate that Notch and Delta could mediate neuron-glia signalling through cell-cell contacts. Such a possibility had already been suggested for the larval brain [25-27]. To further localize Notch activity in the adult brain, we have evaluated the expression of a Notch-reporter construct: Su(H)Bs-lacZ [28].…”

Section: Resultsmentioning

confidence: 99%

Notch Signaling Modulates Sleep Homeostasis and Learning after Sleep Deprivation in Drosophila

et al. 2011

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Summary The role of the trans-membrane receptor Notch in the adult brain is poorly understood. Here, we provide evidence that bunched, a negative regulator of Notch is involved in sleep homeostasis. Genetic evidence indicates that interfering with bunched activity in the mushroom bodies (MBs) abolishes sleep homeostasis. Combining bunched and Delta loss-of-function mutations rescued normal homeostasis, suggesting that Notch signaling may be involved in regulating sensitivity to sleep loss. Preventing the down regulation of Delta by over-expressing a wild-type transgene in MBs reduces sleep homeostasis and, importantly, prevents learning impairments induced by sleep deprivation. Similar resistance to sleep loss is observed with the Notchspl-1 gain-of-function mutants. Immunohistochemistry reveals that the Notch receptor is expressed in glia, whereas Delta is localized in neurons. Importantly the expression of the intracellular domain of Notch, a dominant activated form of the receptor, in glia is sufficient to prevent learning deficits after sleep deprivation. Together these results identify a novel neuronal-glia signalling pathway dependent on Notch and regulated by bunched. These data highlight the emerging role of neuron-glia interactions in regulating both sleep and learning impairments associated with sleep loss.

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Section: Resultsmentioning

confidence: 99%

Notch Signaling Modulates Sleep Homeostasis and Learning after Sleep Deprivation in Drosophila

et al. 2011

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“…3). Although expression of Notch ligands in auditory nerve fibers has not been reported, Delta has been shown to be trafficked to neurites of other neurons, such as Drosophila ventral nerve cord neurons (Cornbrooks et al 2007). Notch signaling has also been shown to be involved in regulating contact-dependent neurite growth in mammalian neuronal cultures (Franklin et al 1999; Sestan et al 1999), suggesting that Delta-Notch signaling occurs in some neurites.…”

Section: Discussionmentioning

confidence: 99%

Hes5 Expression in the Postnatal and Adult Mouse Inner Ear and the Drug-Damaged Cochlea

Hartman

Basak

Nelson

et al. 2009

JARO

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The Notch signaling pathway is known to have multiple roles during development of the inner ear. Notch signaling activates transcription of Hes5, a homologue of Drosophila hairy and enhancer of split, which encodes a basic helix-loop-helix transcriptional repressor. Previous studies have shown that Hes5 is expressed in the cochlea during embryonic development, and loss of Hes5 leads to overproduction of auditory and vestibular hair cells. However, due to technical limitations and inconsistency between previous reports, the precise spatial and temporal pattern of Hes5 expression in the postnatal and adult inner ear has remained unclear. In this study, we use Hes5-GFP transgenic mice and in situ hybridization to report the expression pattern of Hes5 in the inner ear. We find that Hes5 is expressed in the developing auditory epithelium of the cochlea beginning at embryonic day 14.5 (E14.5), becomes restricted to a particular subset of cochlear supporting cells, is downregulated in the postnatal cochlea, and is not present in adults. In the vestibular system, we detect Hes5 in developing supporting cells as early as E12.5 and find that Hes5 expression is maintained in some adult vestibular supporting cells. In order to determine the effect of hair cell damage on Notch signaling in the cochlea, we damaged cochlear hair cells of adult Hes5-GFP mice in vivo using injection of kanamycin and furosemide. Although outer hair cells were killed in treated animals and supporting cells were still present after damage, supporting cells did not upregulate Hes5-GFP in the damaged cochlea. Therefore, absence of Notch-Hes5 signaling in the normal and damaged adult cochlea is correlated with lack of regeneration potential, while its presence in the neonatal cochlea and adult vestibular epithelia is associated with greater capacity for plasticity or regeneration in these tissues; which suggests that this pathway may be involved in regulating regenerative potential.

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“…An alternative approach could be to take advantage of expression lines that selectively label only distinct tracts or commissures. These will most likely become available over time due to the continuous effort of screening for postembryonic lineages (Truman et al, 2004; Cornbrooks et al, 2007; Boone and Doe, 2008) and the invention of novel screening strategies (Pfeiffer et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Muscle strength, functional performance, and self‐reported outcomes four years after arthroscopic partial meniscectomy in middle‐aged patients

Ericsson¹,

Roos²,

Dahlberg³

2006

Arthritis & Rheumatism

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Objective. To examine thigh muscle strength, functional performance, and self-reported outcome in patients with nontraumatic meniscus tears 4 years after operation, and to study the impact of a strength deficit on self-reported outcome and evaluate the feasibility of 3 performance tests in this patient group. Methods. The study group comprised 45 patients (36% women, mean age 46.7) who had an arthroscopic partial meniscectomy a mean of 4 years (range 1-6 years) previously. Main outcome measures included isokinetic strength of knee extensors and flexors, functional performance (1-leg hop, 1-leg rising, and square-hop tests), and a self-reported questionnaire (Knee Injury and Osteoarthritis Outcome Score). Results. We found lower knee extensor strength and worse 1-leg rising capacity in the operated leg, but no difference between operated and nonoperated leg for knee flexors (P < 0.004 and P > 0.3, respectively). Patients with a stronger quadriceps of the operated leg compared with the nonoperated leg had less pain and better function and quality of life (r ‫؍‬ 0.4 -0.6, P < 0.010). We found the 1-leg rising and 1-leg hop tests to be suitable performance tests in middle-aged meniscectomy patients. Conclusion. Quadriceps strength is reduced in the meniscectomized leg compared with the nonoperated leg 4 years after surgery. This relative quadriceps weakness significantly affects objective and self-reported knee function, pain, and quality of life, indicating the importance of restoring muscle function after meniscectomy in middle-aged patients.

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Delta expression in post‐mitotic neurons identifies distinct subsets of adult‐specific lineages in Drosophila

Cited by 10 publications

References 41 publications

Notch Signaling Modulates Sleep Homeostasis and Learning after Sleep Deprivation in Drosophila

Notch Signaling Modulates Sleep Homeostasis and Learning after Sleep Deprivation in Drosophila

Hes5 Expression in the Postnatal and Adult Mouse Inner Ear and the Drug-Damaged Cochlea

Muscle strength, functional performance, and self‐reported outcomes four years after arthroscopic partial meniscectomy in middle‐aged patients

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