Delta-aminolevulinate dehydratase (δ-ALA-D) activity in diabetes and hypothyroidism

Souza, João Baptista D'andrea; Rocha, João Batista Teixeira da; Nogueira, Cristina W.; Borges, Vanessa C.; Kaizer, Rosilene Rodrigues; Morsch, Vera Maria; Dressler, Valderi L.; Martins, Ayrton F.; Flores, Érico M.M.; Schetinger, Maria Rosa Chitolina

doi:10.1016/j.clinbiochem.2006.11.016

Cited by 17 publications

(18 citation statements)

References 25 publications

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“…In this study, the ALA-D activity increased in quails aflatoxin-intoxicated, an enzyme sensible to oxidants [32]. Thus, we believe that ALA-D activity increased due to its relation to oxidative stress, such as observed in this study.…”

Section: Discussionsupporting

confidence: 49%

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

Migliorini

Silva

Santúrio

et al. 2017

Acta Scientiae. Vet.

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Background: Contamination of crops with aflatoxin is considered a serious global threat to food safety, since potent carcinogenic, teratogenic, mutagenic and immunosuppressive effects of aflatoxins are well recognized. Recently, the use of adsorbents has been linked with protective effects against oxidative stress in several diseases. Thus, the aim of this study was to assess the occurrence of oxidative stress in quails (Coturnix coturnix) fed with aflatoxin-contaminated diet, as well as the protective effect of an adsorbent. Materials, Methods & Results: Twenty-eight quails were divided into four groups (n = 7): diet without additives (control; the group A), diet and adsorbent containing aluminosilicates (the group B), aflatoxin-contaminated diet (200 ppb) (the group C), and aflatoxin-contaminated diet (200 ppb) and adsorbent containing aluminosilicates (the group D). The composition of the adsorbent containing aluminosilicates was 0.3% based on yeast cell wall, silymarin, and bentonite. The animals received feed and water ad libitum during 20 days. At the end of the experimental period, total blood was collected by cardiac puncture in tubes without anticoagulant to obtain serum (centrifuged at 3500 g during 10 min) for later determination of biochemical parameters. The liver was placed in a solution of Tris-HCl 10 mM, pH 7.4 for TBARS (Thiobarbituric acid reactive substances), ROS (Reactive oxygen species), SOD (Superoxide dismutase) and CAT (Catalase) analysis. The hepatic tissue was gently homogenized in a glass potter in specific buffer, homogenated, and centrifuged at 10.000 g at 4ºC for 10 min to yield a supernatant (S1) used for analyses. Homogenate aliquots were stored at -80°C until utilization. Fragments of liver and intestine (5 cm) were collected for histopathological analyses. Between days 15 to 20 of the experiment, group C quails showed clinical signs, such as apathy, creepy feathers and reduced feed intake. At day 20 of experiment, macroscopically, the liver of quails belonging to the group C showed greenish yellow color differently from the other groups. Microscopically, no alterations were observed in the liver of animals in groups A and B. Severe diffuse microvacuolar degeneration (hydropic) of hepatocytes and small foci of necrosis in the liver were observed in the group C, as observed in the group D, but in a more moderate degree to microvacuolar degeneration. Seric total protein, albumin, globulin and uric acid levels decreased in the group C and D. The levels of alanine aminotransferase (ALT) increased in the group C, and the treatment with adsorbent was able to avoid this increment. Seric and hepatic reactive oxygen species and TBARS increased in the group C, and the treatment with adsorbent reduced theses parameters in the group D. Catalase (CAT) activity decreased, while ALA-D increased in the group C. The treatment with adsorbent was able to prevent CAT activity decrease, but it did not prevent the increase in ALA-D activity. Discussion: Aflatoxins are considered one of the mo...

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Section: Discussionsupporting

confidence: 49%

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

Migliorini

Silva

Santúrio

et al. 2017

Acta Scientiae. Vet.

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“…Inhibition of δ‐ALAD could impair heme biosynthesis and favors aminolevulinic acid accumulation thus exacerbating oxidative stress in diabetic animal. This finding is consistent with studies in human and animal models of diabetes where a reduced δ‐ALAD activity was observed (Fernández‐Cuartero et al, ; Souza et al, ). Furthermore, ROS‐induced glycation of the lysine residue of the enzyme's active site as well as oxidation of its critical cysteinyl residues could be responsible for the reduced δ‐ALAD activities (Brito, Folmer, Soares, Silveira, & Rocha, ; Caballero, Gerez, Polo, Vazquez, & Batlle, ).…”

Section: Discussionsupporting

confidence: 92%

“…Inhibition of δ-ALAD could impair heme biosynthesis and favors aminolevulinic acid accumulation thus exacerbating oxidative stress in diabetic animal. This finding is consistent with studies in human and animal models of diabetes where a reduced δ-ALAD activity was observed(Fernández-Cuartero et al, 1999;Souza et al, 2007). Furthermore, ROS-induced glycation of the lysine residue of the enzyme's active site as well as oxidation of its critical cysteinyl residues could be responsible for the reduced δ-ALAD activities(Brito, Folmer, Soares, Silveira, & Rocha, 2007;Caballero, Gerez, Polo, Vazquez, & Batlle, 1998).However, the reversal (increased activity) in the δ-ALAD activity in diabetic animals maintained on diet supplemented with the leaves suggests possible modulatory tendency of the diet; which might associated with the antioxidant effect of the jute leaf as evident in increased serum catalase and SOD activities in diabetic rats treated with the leaf-supplemented diet.…”

supporting

confidence: 92%

Dietary supplementation of jute leaf (Corchorus olitorius) modulates hepatic delta‐aminolevulinic acid dehydratase (δ‐ALAD) activity and oxidative status in high‐fat fed/low streptozotocin‐induced diabetic rats

et al. 2019

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Impaired liver function is associated with decreased hepatic delta‐aminolevulinic acid dehydratase (δ‐ALAD) activity in diabetes mellitus. Hence, this study described the effect of dietary jute leaf (Corchorus olitorius) on hepatic δ‐ALAD activity in high‐fat fed combined with low‐dose streptozotocin administered diabetic rats. Animals were fed diets containing 35% fat for 14 days prior to a single administration of low‐dose (35 mg/kg body weight) streptozotocin to induce diabetes. Thereafter, the animals were randomly placed in groups and fed 100 mg/g jute leaf‐supplemented diets for 30 days. The result showed that jute leaf supplementation significantly (p < 0.05) reversed the decreased hepatic δ‐ALAD activity, increased hepatic catalase and SOD activity accompanying the decrease in serum AST and AST activities. This finding suggests that restoration of hepatic δ‐ALAD activity, modulation of hepatic function biomarkers, and increase in antioxidant status could be possible underlying events mediating the hepatoprotective effect of jute leaf in diabetic conditions. Practical applications Decrease in hepatic δ‐ALAD activity has been associated with diabetes‐induced hepatotoxicity arising from prolonged and uncontrolled hyperglycemia. Therefore, increased δ‐ALAD activity represents improved hepatic function in diabetic situations. Antidiabetic properties of jute leaf have been demonstrated but information on its effect on hepatic δ‐ALAD is lacking. Thus, this study revealed that dietary supplementation of jute leaf restored hepatic δ‐ALAD activities and improved liver antioxidant status in diabetic rats which is an indication of its hepatoprotective properties.

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“…Insulin signaling and insulin-like growth factor 1 receptor activation, meanwhile, are known to modulate the levels of enzymes regulating numerous cellular processes. When wild type mice or cultured endothelial cells are exposed to high levels of glucose to establish diabetes-associated conditions, the transcriptional activity of superoxide dismutase 1 ( Sod1 ) and the enzymatic activity of delta-aminolevulinate dehydratase (ALAD) are significantly lower than in controls [20-22]. Levels of glutathione and the enzyme glutathione reductase (GSR) are depleted in apoprotein E-deficient mutant mice [23].…”

Section: Introductionmentioning

confidence: 99%

Rapamycin increases oxidative stress response gene expression in adult stem cells

Kofman

McGraw

Payne³

2012

Aging

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Balancing quiescence with proliferation is of paramount importance for adult stem cells in order to avoid hyperproliferation and cell depletion. In some models, stem cell exhaustion may be reversed with the drug rapamycin, which was shown can suppress cellular senescence in vitro and extend lifespan in animals. We hypothesized that rapamycin increases the expression of oxidative stress response genes in adult stem cells, and that these gene activities diminish with age. To test our hypothesis, we exposed mice to rapamycin and then examined the transcriptome of their spermatogonial stem cells (SSCs). Gene expression microarray analysis revealed that numerous oxidative stress response genes were upregulated upon rapamycin treatment, including superoxide dismutase 1, glutathione reductase, and delta-aminolevulinate dehydratase. When we examined the expression of these genes in 55-week-old wild type SSCs, their levels were significantly reduced compared to 3-week-old SSCs, suggesting that their downregulation is coincident with the aging process in adult stem cells. We conclude that rapamycin-induced stimulation of oxidative stress response genes may promote cellular longevity in SSCs, while a decline in gene expression in aged stem cells could reflect the SSCs' diminished potential to alleviate oxidative stress, a hallmark of aging.

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Delta-aminolevulinate dehydratase (δ-ALA-D) activity in diabetes and hypothyroidism

Cited by 17 publications

References 25 publications

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

The Protective Effects of an Adsorbent against Oxidative Stress in Quails Fed Aflatoxin-Contaminated Diet

Dietary supplementation of jute leaf (Corchorus olitorius) modulates hepatic delta‐aminolevulinic acid dehydratase (δ‐ALAD) activity and oxidative status in high‐fat fed/low streptozotocin‐induced diabetic rats

Rapamycin increases oxidative stress response gene expression in adult stem cells

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