Abstract:We have recently demonstrated that the anthocyanidin delphinidin (DEL), one of the most abundant dietary flavonoids, inhibits activation of ErbB and vascular endothelial growth factor receptor family members. These receptors play crucial roles in the context of tumor progression and the outgrowth of blood and lymphatic vessels. Here, we have developed an improved chemical synthesis for DEL in order to study the effects of the aglycon and its degradation product gallic acid (GA) on endothelial and tumor cells i… Show more
“…Recently, it was demonstrated that delphinidin blocks the proliferation of primary human blood and lymphatic endothelial cells as well as carcinoma cells in vitro in a dose-dependent manner. At higher concentrations, apoptosis of endothelial and tumor cells was induced [25].…”
Section: Resultsmentioning
confidence: 99%
“…Interestingly, in another study in vivo administration of delphinidin significantly reduced angiogenesis in the MT-450 rat syngeneic breast tumor model, although tumor growth and metastasis were promoted. The antiproliferative effect of delphinidin on cultured cells does not necessarily reflect the response of tumors to this anthocyanidin in vivo [25].…”
Cornus mas L. (Cornelian cherry) is a flowering plant indigenous to Europe and parts of Asia, mostly studied for the antimicrobial activity of its juice. In this report, we investigated the composition and the in vitro antioxidant capacity of Cornus mas L. fruit juice from Greece, as well as its antiproliferative properties in vitro and in vivo. The fruits showed a high content of citric, malic, and succinic acid, in contrast to their juice, which had a low concentration of organic acids. The juice demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and modest antiproliferative potential against four human cancer cells lines and one murine: mammary adenocarcinoma MCF-7, hepatocellular carcinoma HepG2 and colon adenocarcinomas Caco2, HT-29, as well as murine colon carcinoma CT26. Cell viability was reduced by 40–50% following incubation of the cells with the highest concentration of the juice. Although Cornelian cherry juice exhibited in vitro growth inhibitory effects against colon carcinoma cells, no tumor growth inhibition was observed in an in vivo experimental colon carcinoma model in mice following prophylactic oral administration of a daily dose of 100 μL juice for a period of 10 days. Thus, our findings raise interesting questions for further research on Cornus mas L. fruit juice, and in parallel, the strong antioxidant potential implies that the plant could be further explored and exploited for its protective effect against oxidative damage.
“…Recently, it was demonstrated that delphinidin blocks the proliferation of primary human blood and lymphatic endothelial cells as well as carcinoma cells in vitro in a dose-dependent manner. At higher concentrations, apoptosis of endothelial and tumor cells was induced [25].…”
Section: Resultsmentioning
confidence: 99%
“…Interestingly, in another study in vivo administration of delphinidin significantly reduced angiogenesis in the MT-450 rat syngeneic breast tumor model, although tumor growth and metastasis were promoted. The antiproliferative effect of delphinidin on cultured cells does not necessarily reflect the response of tumors to this anthocyanidin in vivo [25].…”
Cornus mas L. (Cornelian cherry) is a flowering plant indigenous to Europe and parts of Asia, mostly studied for the antimicrobial activity of its juice. In this report, we investigated the composition and the in vitro antioxidant capacity of Cornus mas L. fruit juice from Greece, as well as its antiproliferative properties in vitro and in vivo. The fruits showed a high content of citric, malic, and succinic acid, in contrast to their juice, which had a low concentration of organic acids. The juice demonstrated significant antioxidant activity against the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and modest antiproliferative potential against four human cancer cells lines and one murine: mammary adenocarcinoma MCF-7, hepatocellular carcinoma HepG2 and colon adenocarcinomas Caco2, HT-29, as well as murine colon carcinoma CT26. Cell viability was reduced by 40–50% following incubation of the cells with the highest concentration of the juice. Although Cornelian cherry juice exhibited in vitro growth inhibitory effects against colon carcinoma cells, no tumor growth inhibition was observed in an in vivo experimental colon carcinoma model in mice following prophylactic oral administration of a daily dose of 100 μL juice for a period of 10 days. Thus, our findings raise interesting questions for further research on Cornus mas L. fruit juice, and in parallel, the strong antioxidant potential implies that the plant could be further explored and exploited for its protective effect against oxidative damage.
“…[8][9][10] Most protease methods utilize combinations of different collagenases and DNase. There are several products that are commercially available including dispase, 11 liberase, [12][13][14][15][16] elastase, 17,18 and purified collagenases. 8,[19][20][21] The targets of these enzymes and reaction conditions are described in Table 1.…”
The immune system has evolved as a powerful tool for our body to combat infections, and is being engineered for new treatments in cancer, autoimmune disease, and trauma.
“…Regarding the mechanism of anti‐cancer effects, both the inhibition of receptor tyrosine kinases like the epidermal growth factor receptor (EGFR) and the catalytic inhibition of topoisomerases might contribute to this effect . However, there is also some contradiction, as the application of DEL as a free aglycon has been found to promote tumor growth and metastasis formation in rats .…”
Our data indicate that a respective polyphenol-rich diet might aid to protect against genotoxic damages caused by AOH, whereby bioactive concentrations of DEL are predominantly expected locally in the intestines.
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