DELLA functions evolved by rewiring of associated transcriptional networks

Briones‐Moreno, Asier; Hernández-García, Jorge; Vargas-Chávez, Carlos; Blanco‐Touriñán, Noel; Phokas, Alexandros; Úrbez, Cristina; Cerdán, Pablo D.; Coates, Juliet C.; Alabadı́, David; Blázquez, Miguel Á.

doi:10.1038/s41477-023-01372-6

Cited by 7 publications

(4 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In seed plants, the protein network featuring DOG1 includes important regulators of reproductive development such as, for example, DELLA and MOTHER OF FT and TFL1 (MFT) (Ali et al., 2022; Xi et al., 2010). DELLA proteins are well‐known for interacting with many other proteins, mainly transcription factors, to regulate various developmental processes (Briones‐Moreno et al., 2023; Lantzouni et al., 2020; Marin‐de la Rosa et al., 2014). For example, in Arabidopsis, DELLAs interact with ABA‐INSENSITIVE 3 (ABI3) and ABI5 to activate transcription of SOMNUS (SOM), repressing seed germination at high temperatures (Lim et al., 2013).…”

Section: Discussionmentioning

confidence: 99%

A DELAY OF GERMINATION 1 (DOG1)‐like protein regulates spore germination in the moss Physcomitrium patens

Vollmeister,

Phokas,

Meyberg

et al. 2023

The Plant Journal

Self Cite

View full text Add to dashboard Cite

SUMMARYDELAY OF GERMINATION 1 is a key regulator of dormancy in flowering plants before seed germination. Bryophytes develop haploid spores with an analogous function to seeds. Here, we investigate whether DOG1 function during germination is conserved between bryophytes and flowering plants and analyse the underlying mechanism of DOG1 action in the moss Physcomitrium patens. Phylogenetic and in silico expression analyses were performed to identify and characterise DOG1 domain‐containing genes in P. patens. Germination assays were performed to characterise a Ppdog1‐like1 mutant, and replacement with AtDOG1 was carried out. Yeast two‐hybrid assays were used to test the interaction of the PpDOG1‐like protein with DELLA proteins from P. patens and A. thaliana. P. patens possesses nine DOG1 domain‐containing genes. The DOG1‐like protein PpDOG1‐L1 (Pp3c3_9650) interacts with PpDELLAa and PpDELLAb and the A. thaliana DELLA protein AtRGA in yeast. Protein truncations revealed the DOG1 domain as necessary and sufficient for interaction with PpDELLA proteins. Spores of Ppdog1‐l1 mutant germinate faster than wild type, but replacement with AtDOG1 reverses this effect. Our data demonstrate a role for the PpDOG1‐LIKE1 protein in moss spore germination, possibly alongside PpDELLAs. This suggests a conserved DOG1 domain function in germination, albeit with differential adaptation of regulatory networks in seed and spore germination.

show abstract

Section: Discussionmentioning

confidence: 99%

A DELAY OF GERMINATION 1 (DOG1)‐like protein regulates spore germination in the moss Physcomitrium patens

Vollmeister,

Phokas,

Meyberg

et al. 2023

The Plant Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…We focused on processes regulated by DELLAs and TFs by co-activation that could be conserved in M. polymorpha , such as flavonoid biosynthesis (8, 39, 40). MpDELLA has been shown to interact with orthologs of MpMYB14 (9), and both share common activated transcriptional targets as Mp PAL ( Mp1g05190 ). This led us to hypothesize that MpMYB14 and MpDELLA would work together to engage MpMED15 and promote gene transcription.…”

Section: Resultsmentioning

confidence: 99%

“…In bryophytes, which lack known GID1 receptors, DELLA levels have been suggested to be primarily controlled through transcriptional regulation (8). In contrast to the proposed differences in DELLA regulation among land plants, the intrinsic property to interact with TFs is conserved in all land plant lineages and likely represents an ancestral function of these (9). Depending on the interacting partner, DELLAs affect transcription through two main alternative mechanisms: they either hinder the binding of TFs to their target DNA sequences (10, 11), or are recruited to the chromatin by TFs, where they can act as transcriptional coactivators (3, 12, 13).…”

Section: Introductionmentioning

confidence: 95%

DELLA Proteins Recruit the Mediator Complex Subunit MED15 to Co-activate Transcription in Land Plants

Hernández-García,

Serrano-Mislata,

Lozano-Quiles

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

DELLA proteins are negative regulators of the gibberellin response pathway in angiosperms, acting as central hubs that interact with hundreds of transcription factors and regulators to modulate their activities. While the mechanism of transcription factor sequestration by DELLAs to prevent DNA binding to downstream targets has been extensively documented, the mechanism that allows them to act as co-activators remains to be understood. Here, we demonstrate that DELLAs directly recruit the Mediator complex to specific loci in Arabidopsis, facilitating transcription. This recruitment involves DELLA amino-terminal domain and the conserved MED15 KIX domain. Accordingly, partial loss of MED15 function mainly disrupted processes known to rely on DELLA co-activation capacity; including cytokinin-dependent regulation of meristem function and skotomorphogenic response, gibberellin metabolism feedback, and flavonol production. We have also found that the single DELLA protein in the liverwortMarchantia polymorphais capable of recruiting MpMED15 subunits, contributing to transcriptional co-activation. The conservation of Mediator-dependent transcriptional co-activation by DELLA between Arabidopsis and Marchantia implies that this mechanism is intrinsic to the emergence of DELLA in the last common ancestor of land plants.Significance StatementDELLA proteins are plant-specific transcriptional hubs integrating environmental signals with endogenous cues. In order to regulate downstream processes, DELLAs modulate the activity of hundreds of transcription factors and transcriptional regulators in various ways. Here, we describe the molecular mechanism underlying DELLA co-activator function. We show that DELLAs act as transcriptional activators in eukaryotic cells by interacting with the Mediator complex subunit MED15. Mediator function is necessary to regulate a subset of DELLA-regulated responses that are mediated by direct co-activation of DELLA-Transcription factors complexes. We further show that this mechanism is present in bryophyte DELLAs, and thus represents a conserved mechanism of DELLA function in land plants.

show abstract

“…Of notable significance is the involvement of the DELLA proteins that act as master regulators that rewire a multitude of transcriptional networks to control diverse biological responses ( Briones-Moreno et al., 2023 ). One of the Arabidopsis DELLA factors exhibits a propensity to interact with distinct SPLs , yielding disparate outcomes.…”

Section: Major Flowering Pathway Genes In Deciduous Woody Plantsmentioning

confidence: 99%

Regulatory frameworks involved in the floral induction, formation and developmental programming of woody horticultural plants: a case study on blueberries

Song,

Liu,

Zhong

2024

Front. Plant Sci.

View full text Add to dashboard Cite

Flowering represents a crucial stage in the life cycles of plants. Ensuring strong and consistent flowering is vital for maintaining crop production amidst the challenges presented by climate change. In this review, we summarized key recent efforts aimed at unraveling the complexities of plant flowering through genetic, genomic, physiological, and biochemical studies in woody species, with a special focus on the genetic control of floral initiation and activation in woody horticultural species. Key topics covered in the review include major flowering pathway genes in deciduous woody plants, regulation of the phase transition from juvenile to adult stage, the roles of CONSTANS (CO) and CO-like gene and FLOWERING LOCUS T genes in flower induction, the floral regulatory role of GA-DELLA pathway, and the multifunctional roles of MADS-box genes in flowering and dormancy release triggered by chilling. Based on our own research work in blueberries, we highlighted the central roles played by two key flowering pathway genes, FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1, which regulate floral initiation and activation (dormancy release), respectively. Collectively, our survey shows both the conserved and diverse aspects of the flowering pathway in annual and woody plants, providing insights into the potential molecular mechanisms governing woody plants. This paves the way for enhancing the resilience and productivity of fruit-bearing crops in the face of changing climatic conditions, all through the perspective of genetic interventions.

show abstract

DELLA functions evolved by rewiring of associated transcriptional networks

Cited by 7 publications

References 52 publications

A DELAY OF GERMINATION 1 (DOG1)‐like protein regulates spore germination in the moss Physcomitrium patens

A DELAY OF GERMINATION 1 (DOG1)‐like protein regulates spore germination in the moss Physcomitrium patens

DELLA Proteins Recruit the Mediator Complex Subunit MED15 to Co-activate Transcription in Land Plants

Regulatory frameworks involved in the floral induction, formation and developmental programming of woody horticultural plants: a case study on blueberries

Contact Info

Product

Resources

About