Delivery of the HIV-1 Tat protein to dendritic cells by the CyaA vector induces specific Th1 responses and high affinity neutralizing antibodies in non human primates

Mascarell, Laurent; Bauche, Cécile; Fayolle, Catherine; Diop, Ousmane M.; Dupuy, Monique; Nougarede, Nolwenn; Perraut, Ronald; Ladant, Daniel; Leclerc, Claude

doi:10.1016/j.vaccine.2006.02.009

Cited by 22 publications

(12 citation statements)

References 49 publications

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“…The technology's breadth of applications is further illustrated by the range of articles investigating oligonucleotides , small molecules , carbohydrates , and extracellular matrix components . In addition, the number of articles describing biosensor work in clinical support [1008][1009][1010][1011][1012][1013][1014][1015][1016][1017][1018][1019][1020][1021][1022][1023][1024], food/agricultural/veterinary/environmental sciences [1025][1026][1027][1028][1029][1030][1031][1032][1033][1034][1035][1036][1037][1038], and membrane/virus/cell characterization [1039][1040][1041][1042][1043][1044][1045][1046][1047][1048][1049][1050] demonstrate Biacore's emerging contributions in these fields. Biacore's more unusual applications, including the characterization of fibrils and the analysis of crude analytes are summarized in References 1051-1066.…”

Section: Methodsmentioning

confidence: 99%

Survey of the year 2006 commercial optical biosensor literature

Rich

Myszka

2007

J of Molecular Recognition

108

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We identified 1219 articles published in 2006 that described work performed using commercial optical biosensor platforms. It is interesting to witness how the biosensor market is maturing with an increased number of instrument manufacturers offering a wider variety of platforms. However, it is clear from a review of the results presented that the advances in technology are outpacing the skill level of the average biosensor user. While we can track a gradual improvement in the quality of the published work, we clearly have a long way to go before we capitalize on the full potential of biosensor technology. To illustrate what is right with the biosensor literature, we highlight the work of 10 groups who have their eye on the ball. To help out the rest of us who have the lights on but nobody home, we use the literature to address common myths about biosensor technology.

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Section: Methodsmentioning

confidence: 99%

Survey of the year 2006 commercial optical biosensor literature

Rich

Myszka

2007

J of Molecular Recognition

108

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“…Most fusion constructs have been engineered genetically; however, chemical fusion of epitopes to ACT also achieved the same effect [119]. In recent studies, this application was used to generate an HIV Tat–ACT fusion that could elicit Tat-specific antibodies and CD8 T cells in immunized mice [120,121], and a fusion with a malarial parasite epitope that could generate parasite-specific CD8 cells and completely protect mice when used as part of a heterologous prime/boost immunization regimen [122]. Interestingly, this technology was also recently used to fuse Mycobacterium tuberculosis antigens to ACT, and these fusion molecules were able to restimulate IFN-γ production by T cells from TB patients, which could facilitate detection of latent infection from patient blood samples, including those from HIV-infected individuals [123,124].…”

Section: Adenylate Cyclase Toxinmentioning

confidence: 99%

Pertussis Toxin and Adenylate Cyclase Toxin: Key Virulence Factors of Bordetella Pertussis and Cell Biology Tools

2010

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Pertussis toxin and adenylate cyclase toxin are two important virulence factors of Bordetella pertussis, the bacterial cause of the respiratory disease pertussis or whooping cough. In addition to studies on the structure, function and role in pathogenesis of these two toxins, they are both used as cell biology tools for a variety of applications owing to their ability to enter mammalian cells, perform enzymatic activities and modify cell signaling events. In this article, recent data from the research literature that enhance our understanding of the nature of these two toxins, their role in the pathogenesis of B. pertussis infection and disease, particularly in modulating host immune responses, and their use as tools for other areas of research will be outlined.

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“…In addition, evidence exists that natural IgM antibodies reacting with Tat may provide an early initial defense against the pathological effects of Tat after HIV infection and influence the course of AIDS progression [131,132]. Similarly, immunization with Tat elicits antibody responses in rodents, monkeys, and humans able to block the activity of extracellular Tat on cellular entry, gene expression, and replication [4,84,[133][134][135][136][137][138][139][140][141][142]. Moreover, strong anti-Tat antibody responses correlated with an efficient reduction in plasma viremia and long-term protection in Tat protein-vaccinated monkeys challenged intrarectally with an heterologous SHIV virus [143].…”

Section: The Choice Of Tat As Vaccine Relevant Antigenmentioning

confidence: 99%