Delivery of TFPI-2 Using Ultrasound with a Microbubble Agent (SonoVue) Inhibits Intimal Hyperplasia after Balloon Injury in a Rabbit Carotid Artery Model

Zhou, Jingtao; Wang, Yuxue; Xiong, Yang; Wang, Hongxing; Feng, Youmei; Chen, Juan

doi:10.1016/j.ultrasmedbio.2010.08.013

Cited by 19 publications

(16 citation statements)

References 32 publications

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“…For the protein expression analysis, western blot was performed according to the previously described protocol [19]. In brief, after mice were sacrificed, gastric mucosa was harvested and homogenized in 1 mL of lysis buffer containing 50 mM NaCl, 10 mM Tris, 1 mM EDTA, 1 mM PMSF, 0.5 mM Na 3 VO 4 .12H 2 O, 50 mM NaF and 1 mM benzamidine.…”

Section: Methodsmentioning

confidence: 99%

Potential malignant transformation in the gastric mucosa of immunodeficient mice with persistent Mycoplasma penetrans infection

et al. 2017

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Mycoplasma infection has been reported in immunocompromised cancer patients; nevertheless, it is not clear if persistent Mycoplasma infection could facilitate the proliferation of cancer cells in immunocompromised organisms. The aim of this study was to examine the relationship between persistent Mycoplasma infection and malignant transformation in an immunodeficient host model. Immunodeficient mouse model was established using cyclophosphamide and mice gastric mucosal cells were infected with Mycoplasma penetrans (Mpe). After 18 weeks, mice were sacrificed and gastric mucosal Mpe infected cells were identified by fluorescence in situ hybridization (FISH). Moreover, pathological and ultrastructural changes in mice gastric mucosa were evaluated and the expression of multiple proto-oncogenes was examined by Western blot. Our data show that Mpe infection was detected in the blood of immunodeficient mice and Mpe persistent infection in mice gastric mucosa was confirmed by FISH. There were pathological and ultrastructural malignant transformation occurred in the gastric mucosa of infected mice compared to control mice. Mpe infected mice showed lower expression of p53 and p21 and higher H-ras expression compared to the control group. Moreover, expression of NF-κB p65 subunit increased in Mpe infected mice, similar to the TNF-α expression. Bax expression in gastric mucosa of Mpe infected mice was lower while Bcl-2 expression was higher than in the uninfected control group. Collectively these data demonstrate that persistent Mpe infection is associated with aberrant expression of multiple proto-oncogenes in gastric mucosa of immunodeficient mice which potentially facilitate the malignant transformation.

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Section: Methodsmentioning

confidence: 99%

Potential malignant transformation in the gastric mucosa of immunodeficient mice with persistent Mycoplasma penetrans infection

et al. 2017

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“…The combination of ultrasound exposure and microbubbles for gene delivery has several advantages, including low cytotoxicity, high target selectivity, low immunogenicity, and repeatable applicability. Previous reports have indicated that SonoVue could be a useful gene delivery tool 26 . Therefore, we hypothesized that the combination of ultrasound exposure and SonoVue would be a useful tool for the intra‐cellular delivery of NET‐1 siRNA.…”

Section: Discussionmentioning

confidence: 98%

Neuroepithelial Transforming Protein 1 Short Interfering RNA-Mediated Gene Silencing With Microbubble and Ultrasound Exposure Inhibits the Proliferation of Hepatic Carcinoma Cells In Vitro

Han

Cheng

Jing

et al. 2012

Journal of Ultrasound in Medicine

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“…Previous studies used commercially available microbubbles, including SonoVue or Definity microbubbles, as in vivo gene delivery vehicles (28,29). In these studies, plasmid DNA was mixed and incubated with microbubbles to couple the genes to the microbubbles prior to intravenous injection.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound combined with targeted cationic microbubble-mediated angiogenesis gene transfection improves ischemic heart function

Zhou

Deng

et al. 2017

Experimental and Therapeutic Medicine

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The present study aimed to construct targeted cationic microbubbles (TCMBs) by synthesizing cationic microbubbles conjugated to an intercellular adhesion molecule-1 (ICAM-1) antibody, and then to use the TCMBs to deliver the angiopoietin-1 (Ang-1) gene into infarcted heart tissue using ultrasound-mediated microbubble destruction. It was hypothesized that the TCMBs would accumulate in higher numbers than non-targeted cationic microbubbles (CMBs) in the infarcted heart, and would therefore increase the efficiency of targeted Ang-1 gene transfection and promote angiogenesis. The results of the study demonstrated that the ability of TCMBs to target inflammatory endothelial cells was 18.4-fold higher than that of the CMBs in vitro. The accumulation of TCMBs was greater than that of CMBs in TNF-α-stimulated human umbilical cord veins, indicated by a 212% higher acoustic intensity. In vivo, the TCMBs specifically accumulated in the myocardial infarct area in a rabbit model. Three days after ultrasound microbubble-mediated gene transfection, Ang-1 protein expression in the TCMB group was 2.7-fold higher than that of the CMB group. Angiogenesis, the thickness of the infarct region and the heart function of the TCMB group were all significantly improved compared with those in the CMB and control groups at 4 weeks following gene transfection (all P<0.01). Therefore, the results of the current study demonstrate that ultrasound-mediated TCMB destruction effectively delivered the Ang-1 gene to the infarcted myocardium, resulting in improved cardiac morphology and function in the animal model. Ultrasound-mediated TCMB destruction is a promising strategy for improving gene therapy in the future.

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Delivery of TFPI-2 Using Ultrasound with a Microbubble Agent (SonoVue) Inhibits Intimal Hyperplasia after Balloon Injury in a Rabbit Carotid Artery Model

Cited by 19 publications

References 32 publications

Potential malignant transformation in the gastric mucosa of immunodeficient mice with persistent Mycoplasma penetrans infection

Potential malignant transformation in the gastric mucosa of immunodeficient mice with persistent Mycoplasma penetrans infection

Neuroepithelial Transforming Protein 1 Short Interfering RNA-Mediated Gene Silencing With Microbubble and Ultrasound Exposure Inhibits the Proliferation of Hepatic Carcinoma Cells In Vitro

Ultrasound combined with targeted cationic microbubble-mediated angiogenesis gene transfection improves ischemic heart function

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