Delivery of siRNA into the cytoplasm by liposomal bubbles and ultrasound

Negishi, Yoichi; Endo, Yoko; Fukuyama, Tetsuya; Suzuki, Ryo; Takizawa, Tomoko; Omata, Daiki; Maruyama, Kazuo; Aramaki, Yukihiko

doi:10.1016/j.jconrel.2008.08.019

Cited by 115 publications

(83 citation statements)

References 37 publications

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“…The liposomes were prepared by a reverse-phase evaporation method, as described previously. 4) In brief, all the reagents were dissolved in 1 : 1 (v/v) chloroform-diisopropylether. Phosphate-buffered saline was added to the lipid solution, and the mixture was sonicated and then evaporated at 47°C.…”

Section: Preparation Of Liposomes and Blsmentioning

confidence: 99%

“…These are polyethylene glycol (PEG)-modified liposomes that contain echo-contrast gas, which can function as a gene and short interfering RNA (siRNA) delivery tool with US exposure in vitro and in vivo. 3,4) However, it is not yet known whether BLs increase the temperature of tumor tissue and enhance the ablation area and antitumor effect under HIFU exposure as microbubbles. In this study, we examined whether BLs and HIFU could enhance the ablation area of tumors and the antitumor effect.…”

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confidence: 99%

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Combination of Bubble Liposomes and High-Intensity Focused Ultrasound (HIFU) Enhanced Antitumor Effect by Tumor Ablation

Hamano

Negishi

Takatori

et al. 2014

Biological & Pharmaceutical Bulletin

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Ultrasound (US) is used in the clinical setting not only for diagnosis but also for therapy. As a therapeutic US technique, high-intensity focused ultrasound (HIFU) can be applied to treat cancer in a clinical setting. Microbubbles increased temperature and improved the low therapeutic efficiency under HIFU; however, microbubbles have room for improvement in size, stability, and targeting ability. To solve these issues, we reported that "Bubble liposomes" (BLs) containing the US imaging gas (perfluoropropane gas) liposomes were suitable for ultrasound imaging and gene delivery. In this study, we examined whether BLs and HIFU could enhance the ablation area of the tumor and the antitumor effect. First, we histologically analyzed the tumor after BLs and HIFU. The ablation area of the treatment of BLs and HIFU was broader than that of HIFU alone. Next, we monitored the temperature of the tumor, and examined the antitumor effect. The temperature increase with BLs and HIFU treatment was faster and higher than that with HIFU alone. Moreover, treatment with BLs and HIFU enhanced the antitumor effect, which was better than with HIFU alone. Thus, the combination of BLs and HIFU could be efficacious for cancer therapy.Key words high-intensity focused ultrasound; tumor ablation; bubble liposome; cancer therapy Ultrasound (US) imaging is a widely used diagnostic technique that allows for real-time imaging and combines the advantages of noninvasiveness with easy access by the public and low cost. In addition, US is used in the clinical setting not only for diagnosis but also for therapy. Recently, as a therapeutic US technique, high-intensity focused ultrasound (HIFU) has been applied for the treatment of prostate cancer and liver cancer in a clinical setting.

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Section: Preparation Of Liposomes and Blsmentioning

confidence: 99%

mentioning

confidence: 99%

Combination of Bubble Liposomes and High-Intensity Focused Ultrasound (HIFU) Enhanced Antitumor Effect by Tumor Ablation

Hamano

Negishi

Takatori

et al. 2014

Biological & Pharmaceutical Bulletin

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“…Dilution of silencing RNA in BPS in volume of 1 ml resulted in flush after an alternated administration of 0.3 ml of BPS for 24-36 hours and protein knockdown in hydrodynamic delivery (Kinoshita et al, 2005). Tissues containing silencing RNA may be used in the possible heart transplantation in animal models (Negishi et al, 2008). In comparative studies it was observed that silencing RNA labeled with fluorescent administered rectal and intra-peritoneal significant uptake was observed in the rectal administration after 4 hours of the dosing (Shukla et al, 2009).…”

Section: Modalities For Delivering Silencing Rna To Kidneymentioning

confidence: 99%

Remedial applications of silencing ribonucleic acids and modalities for its delivery to the kidneys - A review

Wang¹,

Lv²,

Zhu³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

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Background: The Kidney has been the target organ for the delivery of silencing ribonucleic acids (silencing RNA) administered systemically in comparison to other body tissues. Materials and method:In this review, we discussed different approaches made to delivering proteins to the kidneys in different conditions like normal and pathological defects. Data from clinical experiments have been used to discuss and support the administration of silencing RNA for the treatment of kidney diseases. Results: Results were achieved using the available genome wide RNA libraries. Conclusion:The research results are helpful in application to 3D and conventional models to find the involvement of signal pathways in kidney diseases.

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“…URDDS include microbubbles, [8][9][10] nanobubbles, 11,12 nanodroplets, 13 liposomes, 14,15 emulsion, 16 and micelles. [17][18][19] A combination of two or more formulations can be used as URDDS, such as liposomal bubbles 20,21 and microemulsions. 22 The drugs loaded can include inorganic substances, eg, titanium dioxide, 23 small molecules (curcumin, 24 doxorubicin, 25 cisplatin, 5 epirubicin hydrochloride, 26 10-hydroxycamptothecin 27 ), proteins, 28,29 small interfering RNA, 8,21 DNA, 30 and antisense oligodeoxynucleotides.…”

Section: Introductionmentioning

confidence: 99%

“…[17][18][19] A combination of two or more formulations can be used as URDDS, such as liposomal bubbles 20,21 and microemulsions. 22 The drugs loaded can include inorganic substances, eg, titanium dioxide, 23 small molecules (curcumin, 24 doxorubicin, 25 cisplatin, 5 epirubicin hydrochloride, 26 10-hydroxycamptothecin 27 ), proteins, 28,29 small interfering RNA, 8,21 DNA, 30 and antisense oligodeoxynucleotides. 31,32 Their therapeutic applications include anticancer therapy, 23,33 treatment of ischemic myocardium, 29 induction of an immune response, 34 cartilage tissue engineering, 35 transdermal drug delivery, 36,37 treatment of Huntington's disease 38 and thrombolysis, 39,40 and disruption of the blood-brain barrier.…”

Section: Introductionmentioning

confidence: 99%

Potential and problems in ultrasound-responsive drug delivery systems

Zhao¹,

Du²,

Lu³

et al. 2013

IJN

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Abstract:Ultrasound is an important local stimulus for triggering drug release at the target tissue. Ultrasound-responsive drug delivery systems (URDDS) have become an important research focus in targeted therapy. URDDS include many different formulations, such as microbubbles, nanobubbles, nanodroplets, liposomes, emulsions, and micelles. Drugs that can be loaded into URDDS include small molecules, biomacromolecules, and inorganic substances. Fields of clinical application include anticancer therapy, treatment of ischemic myocardium, induction of an immune response, cartilage tissue engineering, transdermal drug delivery, treatment of Huntington's disease, thrombolysis, and disruption of the blood-brain barrier. This review focuses on recent advances in URDDS, and discusses their formulations, clinical application, and problems, as well as a perspective on their potential use in the future.

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Delivery of siRNA into the cytoplasm by liposomal bubbles and ultrasound

Cited by 115 publications

References 37 publications

Combination of Bubble Liposomes and High-Intensity Focused Ultrasound (HIFU) Enhanced Antitumor Effect by Tumor Ablation

Combination of Bubble Liposomes and High-Intensity Focused Ultrasound (HIFU) Enhanced Antitumor Effect by Tumor Ablation

Remedial applications of silencing ribonucleic acids and modalities for its delivery to the kidneys - A review

Potential and problems in ultrasound-responsive drug delivery systems

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