Delivery of siRNA into breast cancer cells via phage fusion protein-targeted liposomes

Abstract

Efficacy of siRNAs as potential anticancer therapeutics can be increased by their targeted delivery into cancer cells via tumor-specific ligands. Phage display offers an unique approach to identify highly specific and selective ligands that can deliver nanocarriers to the site of disease. In this study, we proved a novel approach for intracellular delivery of siRNAs into breast cancer cells through their encapsulation into liposomes targeted to the tumor cells with preselected intact phage proteins. The targeted siRNA liposomes were obtained by a fusion of two parental liposomes containing spontaneously inserted siRNA and fusion phage proteins. The presence of pVIII coat protein fused to a MCF-7 cell-targeting peptide DMPGTVLP in the liposomes was confirmed by Western blotting. The novel phage-targeted siRNA-nanopharmaceuticals demonstrate significant down-regulation of PRDM14 gene expression and PRDM14 protein synthesis in the target MCF- 7 cells. This approach offers the potential for development of new anticancer siRNA-based targeted nanomedicines.

Immunocompatibility of Bacteriophages as Nanomedicines

“…Recently, Bedi et al, demonstrated phage-targeted siRNA nanopharmaceuticals through their encapsulation into liposomes targeted to the breast tumor cells with preselected intact phage proteins [20]. "The presence of pVIII coat protein fused to a MCF-7 cell-targeting peptide "DMPGTVLP" in the liposomes was confirmed by Western blotting.…”

Synthetic Biology & Bioinformatics Prospects in the Cancer Arena

“…In a different approach, a phage display-selected ligand targeting breast cancer cells was incorporated in liposomes containing siRNA. The delivered liposomes were shown to significantly downregulate the PRDM14 gene in the MCF7 target cells (Bedi et al, 2011). In addition, the option to directly use bacteriophages as drug-delivery platforms has been explored.…”

Adsorption Isotherms and Kinetics of Multi-Walled Carbon Nanotubes (MWCNTs), Boron Nitride Nanotubes (BNNTs), Amorphous Boron Nitride Nanotubes (a-BNNTs) and Hexagonal Boron Nitride Nanotubes (h-BNNTs) for Eliminating Carcinoma, Sarcoma, Lymphoma, Leukemia, Germ Cell Tumor and Blastoma Cancer Cells and Tissues

“…Multi-Walled Carbon Nanotubes (MWCNTs), Boron Nitride Nanotubes (BNNTs), Amorphous Boron Nitride Nanotubes (a-BNNTs) and Hexagonal Boron Nitride Nanotubes (h-BNNTs) for eliminating carcinoma, sarcoma, lymphoma, leukemia, germ cell tumor and blastoma cancer cells and tissues from human blood are a new form of nanotubes with unique characteristics such as small size, high area specific surface, a hollow tube-like structure, high mechanical strength, and considerable electrical conductivity [164][165][166][167][168][169][170][171][172][173]. Nanotubes are classified as single-walled (SWNTs) and multi-walled (MWNTs).…”

“…They can show a behavior similar to that of metals or semi-conductors depending on their diameter and characteristics. These behaviors usually originate from the development and arrangement of their Nanolayers [164][165][166][167][168][169][170][171][172][173].…”

“…Studies have evaluated the application of these nanomaterials for the removal of contaminants such as micropollutants, heavy metals, radio-nucleotides, chemical organic compounds and hazardous compounds from gas currents . Multi-Walled Carbon Nanotubes (MWCNTs), Boron Nitride Nanotubes (BNNTs), Amorphous Boron Nitride Nanotubes (a-BNNTs) and Hexagonal Boron Nitride Nanotubes (h-BNNTs) can also be used in the fabrication of transistors, field emission screens, Nano-lithography and biosensors [158][159][160][161][162][163][164][165][166][167][168][169][170][171][172][173].…”