Delivery of Recombinant Human Bone Morphogenetic Protein-2 Using a Compression-Resistant Matrix in Posterolateral Spine Fusion in the Rabbit and in the Non-Human Primate

Abstract

The new compression-resistant biphasic ceramic phosphate/collagen sponge matrices were biologically compatible with recombinant human bone morphogenetic protein 2 bone formation, resulted in biomechanically stiffer fusion masses than autograft, better space maintenance than plain collagen sponges, and improved handling and radiographic resorption properties over the ceramic carriers previously tested.

Osteogenic potential of five different recombinant human bone morphogenetic protein adenoviral vectors in the rat

“…Some BMPs can induce bone formation in mice, rats, rabbits, sheep, dogs, monkeys, and baboons. [5][6][7][8][9][10][11][12][13][14][15][16][17][18] Purified BMP-2, BMP-4, and BMP-7 have been used in human clinical cases to repair resistant nonunions and segmental defects of long bones. [19][20][21][22][23] In a previous study, the ability of recombinant human BMP-2, BMP-3, BMP-4, BMP-5, BMP-6, and BMP-7 to stimulate DNA synthesis was compared in cultures of periosteal cells and epiphyseal and sternal chondrocytes of embryonic chick.…”

Comparing ectopic bone growth induced by rhBMP‐2 on an absorbable collagen sponge in rat and rabbit models

“…Previous preclinical experiments with rhBMP-2 implanted in animals showed that successful results with rhBMP-2 were dependent on concentration, not delivered dose. 22 Although it is unknown if longer soaking times would benefit clinical outcomes in an ALIF application, longer soaking times may help to maximize the amount of rhBMP-2 at the local site in environments that may be exposed to increased compressive loads.…”

Mistura de proteínas morfogenéticas ósseas, hidroxiapatita, osso inorgânico e colágeno envolta por membrana de pericárdio no preenchimento de defeito ósseo segmentar em coelhos

“…Existem diferentes tipos de carreadores, com diferentes tipos de resposta (Zegzula et al, 1997;Suh et al, 2002), sendo ainda necessário esclarecer problemas como combinação, biodisponibilidade, difusibilidade e espécie (Lane, 2001). O carreador incorporado ao produto utilizado tratase da hidroxiapatita reabsorvível microgranular que, segundo Granjeiro et al (1992), permite reabsorção pelo mecanismo de fagocitose e possui alto grau de pureza.…”

“…Vários tipos de compostos de hidroxiapatita, reabsorvíveis ou não, têm sido testados como carreadores com resultados satisfatórios (Suh et al, 2002;Den Boer et al, 2003). O mesmo carreador empregado no experimento foi utilizado em associação à BMP (Lima, 2002), ao osso bovino inorgânico (Costa Filho et al, 2001), ou à matriz óssea orgânica (Braz et al, 2003), aparentemente sem interferência com a produção de osso.…”