Delivery of noncarrier naked DNA vaccine into the skin by supersonic flow induces a polarized T helper type 1 immune response to cancer

Abstract

A shift in the immune response to favour enhanced tumor rejection can be achieved by skin delivery of naked DNA vaccine.

RNA interference-mediated silencing of Foxo3 in antigen-presenting cells as a strategy for the enhancement of DNA vaccine potency

“…37,38 However, it is well established that the anti-p185 antibody has an important role in directly inhibiting the growth of HER-2-positive tumors in vitro and in several preclinical models. 33,39 Furthermore, it has also been documented that the collaboration of both the HER-2/neu antibody and T cells contributes to the rejection of HER-2/neu tumors in transgenic mice and the eradication of clinically evident p185neu-positive tumors in wild-type BALB/ mice.…”

An HDAC inhibitor enhances the antitumor activity of a CMV promoter-driven DNA vaccine

“…20 Briefly, for naked DNA vaccination, 1 mg of DNA plasmid dissolved in 20 ml of autoclaved double-distilled water was directly added to the loading hole near the nozzle and then delivered to the shaved abdominal region of mice at a discharge pressure of 60 psi using a lowpressure-accelerated gene gun (GDS-80) (Wealtec Technologies Co. Ltd, Taipei, Taiwan).…”

“…20 In brief, C3H/He mice were inoculated with 1 mg pCMV-EGFP DNA plasmid (pEGFR-N1; Clontech Laboratories, Inc., Palo Alto, CA) with the HDAC inhibitor OSU-HDAC42. Inguinal lymph nodes were collected 48 h after DNA plasmid administration.…”

“…20 Serum immunoglobulin (Ig) G antibody response was carried out with alkaline phosphatase-conjugated rat antimouse IgG secondary antibodies (Calbiochem, Darmstadt, Germany). Color development was facilitated using 3,3 0 ,5,5 0 -tetramethylbenzidine (Sigma).…”

“…20 DNA-vaccinated mice were killed 1 week after the last DNA vaccination, and spleens were isolated and RBCs were removed from single-cell splenocyte suspensions using a NH 4 Cl-KCl lysis buffer. A total of 7 Â 10 6 washed splenocytes was then stimulated with 20 mg ml À1 human recombinant ErbB2 protein in RPMI 1640 medium containing 10% FBS, 20 mg ml À1 streptomycin, 20 U ml À1 penicillin, and 50 mM 2-ME (Life Technologies, Rockville, MD).…”