Delivery of germinal granules and localized RNAs via the messenger transport organizer pathway to the vegetal cortex of Xenopus oocytes occurs through directional expansion of the mitochondrial cloud

Wilk, Katarzyna; Biliński, S; Dougherty, Matthew; Kloc, Małgorzata

doi:10.1387/ijdb.041906kw

Cited by 53 publications

(38 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the METRO pathway, mRNAs accumulate within a region of the Balbiani body, also called the mitochondrial cloud, in stage I oocytes (Chang et al, 2004), and then translocate to the vegetal cortex through a directional expansion of the mitochondrial cloud itself (Wilk et al, 2004). Interestingly, many mRNAs localized through the METRO pathway are also components of the germ plasm and involved in germ cell differentiation (Kloc et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Maternal syntabulin is required for dorsal axis formation and is a germ plasm component in Xenopus

Colozza

Robertis

2014

Differentiation

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Maternal syntabulin is required for dorsal axis formation and is a germ plasm component in Xenopus

Colozza

Robertis

2014

Differentiation

View full text Add to dashboard Cite

“…7). The Balbiani bodydependent RNA transport system is shared by germline-specific RNAs (Kloc et al, 2001;Kloc and Etkin, 1995;Kloc et al, 1996;Kosaka et al, 2007;Wilk et al, 2005). Despite their common localization in early oocytes, important differences exist between syntabulin and germline-specific mRNA localization in late stage oocytes and in embryos.…”

Section: Discussionmentioning

confidence: 99%

“…syntabulin RNA is transported to the vegetal pole through the Buc-mediated Balbiani bodydependent pathway Some vegetal RNAs, including germline-specific gene products, are transported to the vegetal pole through the Balbiani body (Kloc et al, 2001;Kloc and Etkin, 1995;Kloc et al, 1996;Wilk et al, 2005), which requires maternal Buc function for its formation (Bontems et al, 2009;Marlow and Mullins, 2008). As syntabulin mRNA localizes to the vegetal pole of eggs and early embryos, we investigated when syntabulin achieves its vegetal pole localization and whether this involves the Buc-mediated Balbiani bodydependent pathway in oocytes.…”

Section: Microtubule-dependent Transport Of Syntabulinmentioning

confidence: 99%

“…In Xenopus and zebrafish oocytes, mRNAs localize to the oocyte vegetal pole via overlapping transport pathways. The early pathway involves recruitment of mRNAs, including germline-specific transcripts to the Balbiani body (Kloc et al, 2001;Kloc and Etkin, 1995;Kloc et al, 1996;Wilk et al, 2005), an evolutionarily conserved aggregate of organelles and molecules that is present in early oocytes (Guraya, 1979;Kloc et al, 2004). Balbiani body formation is controlled by Bucky ball (Buc).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Syntabulin, a motor protein linker, controls dorsal determination

Nojima¹,

Rothhämel

Shimizu³

et al. 2010

Development

134

View full text Add to dashboard Cite

SUMMARYIn amphibian and teleost embryos, the dorsal determinants (DDs) are believed to be initially localized to the vegetal pole and then transported to the prospective dorsal side of the embryo along a microtubule array. The DDs are known to activate the canonical Wnt pathway and thereby promote the expression of genes that induce the dorsal organizer. Here, by identifying the locus of the maternal-effect ventralized mutant tokkaebi, we show that Syntabulin, a linker of the kinesin I motor protein, is essential for dorsal determination in zebrafish. We found that syntabulin mRNA is transported to the vegetal pole during oogenesis through the Bucky ball (Buc)-mediated Balbiani body-dependent pathway, which is necessary for establishment of animal-vegetal (AV) oocyte polarity. We demonstrate that Syntabulin is translocated from the vegetal pole in a microtubule-dependent manner. Our findings suggest that Syntabulin regulates the microtubule-dependent transport of the DDs, and provide evidence for the link between AV and dorsoventral axis formation.

show abstract

“…Transport of RNA to the vegetal pole via the Bb has been proposed to involve an entrapment and expansion mechanism (Chang et al, 2004;Wilk et al, 2005). A self-organizing and amplifying Buc/Balbiani complex could facilitate robust recruitment (entrapment) of RNP complexes, including those containing buc.…”

Section: Oocyte Polarity and Follicle Cell Fatementioning

confidence: 99%

Oocyte polarity requires a Bucky ball-dependent feedback amplification loop

et al. 2014

View full text Add to dashboard Cite

In vertebrates, the first asymmetries are established along the animal-vegetal axis during oogenesis, but the underlying molecular mechanisms are poorly understood. Bucky ball (Buc) was identified in zebrafish as a novel vertebrate-specific regulator of oocyte polarity, acting through unknown molecular interactions. Here we show that endogenous Buc protein localizes to the Balbiani body, a conserved, asymmetric structure in oocytes that requires Buc for its formation. Asymmetric distribution of Buc in oocytes precedes Balbiani body formation, defining Buc as the earliest marker of oocyte polarity in zebrafish. Through a transgenic strategy, we determined that excess Buc disrupts polarity and results in supernumerary Balbiani bodies in a 3′UTR-dependent manner, and we identified roles for the buc introns in regulating Buc activity. Analyses of mosaic ovaries indicate that oocyte pattern determines the number of animal pole-specific micropylar cells that are associated with an egg via a close-range signal or direct cell contact. We demonstrate interactions between Buc protein and buc mRNA with two conserved RNA-binding proteins (RNAbps) that are localized to the Balbiani body: RNA binding protein with multiple splice isoforms 2 (Rbpms2) and Deleted in azoospermia-like (Dazl). Buc protein and buc mRNA interact with Rbpms2; buc and dazl mRNAs interact with Dazl protein.Cumulatively, these studies indicate that oocyte polarization depends on tight regulation of buc: Buc establishes oocyte polarity through interactions with RNAbps, initiating a feedback amplification mechanism in which Buc protein recruits RNAbps that in turn recruit buc and other RNAs to the Balbiani body.

show abstract

Delivery of germinal granules and localized RNAs via the messenger transport organizer pathway to the vegetal cortex of Xenopus oocytes occurs through directional expansion of the mitochondrial cloud

Cited by 53 publications

References 24 publications

Maternal syntabulin is required for dorsal axis formation and is a germ plasm component in Xenopus

Maternal syntabulin is required for dorsal axis formation and is a germ plasm component in Xenopus

Syntabulin, a motor protein linker, controls dorsal determination

Oocyte polarity requires a Bucky ball-dependent feedback amplification loop

Contact Info

Product

Resources

About