Delivery of DNA vaccine using chitosan–tripolyphosphate (CS/TPP) nanoparticles in Asian sea bass, Lates calcarifer (Bloch, 1790) for protection against nodavirus infection

Vimal, S.; Majeed, Shahid; Nambi, K.S.N.; Madan, N.; Farook, M.A.; Venkatesan, C.; Taju, G.; Venu, S.; Subburaj, R.; Thirunavukkarasu, A. R.; Hameed, A.S. Sahul

doi:10.1016/j.aquaculture.2013.11.017

Cited by 55 publications

(26 citation statements)

References 37 publications

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“…No protection was observed in turbot [346]. Whereas specific anti-NNV antibodies were only detected in grouper and Asian sea bass [347,349], the expression of cell-mediated cytotoxicity-related genes was observed in grouper and European sea bass [348,349], but it was not tested in Asian seabass [347].…”

Section: Preventionmentioning

confidence: 93%

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Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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Section: Preventionmentioning

confidence: 93%

“…Finally, the potential use of DNA vaccines to prevent VER outbreaks has been explored in turbot, grouper, European and Asian seabass [346][347][348][349][350]. The protection obtained was moderate in grouper and European sea bass (RPS: 43%-47% and 45%, respectively) [348,349], but considerably higher in Asian sea bass (RPS 77%) [350].…”

Section: Preventionmentioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

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“…Numerous vaccines have been developed with an aim to control NNV: formalin-inactivated virus (Kai and Chi, 2008;Pakingking et al, 2009;Yamashita et al, 2005), binary ethylenimine (BEI)-inactivated virus (Barnes et al, 2005;Kai and Chi, 2008), recombinant protein (Barnes et al, 2005;Husgard et al, 2001;Lin et al, 2007;Sommerset et al, 2005;Tanaka et al, 2001;Vimal et al, 2014a) and DNA based vaccines (Sommerset et al, 2005;Vimal et al, 2014b). The protective immunity conferred by a given vaccine is most commonly evaluated in trials by the analysis of the relative percent survival (RPS) of vaccinated and nonvaccinated fish.…”

Section: Introductionmentioning

confidence: 99%

Comparison of ELISA formats for detection of antibodies specific for nervous necrosis virus (Betanodavirus) in the serum of immunized barramundi Lates calcarifer and Australian bass Macquaria novemaculeata

et al. 2016

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“…; Vimal, Abdul Majeed et al . ). Then the cells were washed with wash buffer, followed by addition of the goat anti‐rabbit IgG secondary antibody (IgG) conjugated with FITC at a dilution of 1:50 for 45 min at room temperature.…”

Section: Methodsmentioning

confidence: 97%

“…Vaccine is a biologically prepared antigen which helps to improve the immunity in animals against a particular disease or a group of diseases. Different types of vaccines such as heat or formalin killed whole cell vaccine, recombinant protein vaccine and DNA vaccines have been tried to protect the fish from a variety of viral and bacterial diseases (Yuasa, Koesharyani, Roza, Mori, Katata & Nakai ; Sommerset, Lorentzen, Lorenzen, Bleie & Nerland ; Sommerset, Skern, Biering, Bleie, Fiksdal & Grov ; Pakingking, Bautista, Ayson & Reyes ; Oh, Gye & Nishizawa ; Vimal, Abdul Majeed, Nambi, Madan, Farook, Venkatesan, Taju, Venu, Subburaj, Thirunavukkarasu & Sahul Hameed ; Vimal, Madan, Farook, Nambi, Abdul Majeed, Rajkumar, Venu, Thirunavukkarasu & Sahul Hameed ).…”

Section: Introductionmentioning

confidence: 99%

Development, distribution and expression of a DNA vaccine against nodavirus in Asian Seabass,Lates calcarifier(Bloch, 1790)

Vimal¹,

Farook²,

Madan³

et al. 2014

Aquac Res

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Fish nodavirus (betanodavirus), a viral pathogen responsible for viral nervous necrosis (VNN) was isolated from infected Asian sea bass (Lates calcarifer). The distribution, clearance and expression of nodavirus vaccine, on the basis of DNA vaccine (pFNCPE42 DNA-pcDNA3.1) construction, were analysed in tissues of the Asian seabass by PCR, RT-PCR, ELISA and Immunohistochemistry. Fish immunized with a single intramuscular injection of 20 lg of the pFNCPE42-DNA vaccine showed a significant increase in the serum antibody level in the 3rd week after vaccination, compared to control eukaryotic expression vector pcDNA3.1 vaccinated fish. Results from PCR studies indicated that the vaccine-containing plasmids were distributed in heart, intestine, gill, muscle and liver 10 days after vaccination. Clearance of pFNCPE42-DNA vaccine was studied at 10, 25, 50, 75 and 100 days of post vaccination (d p.v). At 100 days p.v. pFNCPE42-DNA was cleared from muscle of vaccinated sea bass. In vitro and in vivo expression of fish nodavirus capsid protein gene (FNCP) was determined by fluorescent microscopy. Asian seabass was immunized with pFNCPE42-DNA vaccine at a dose of 20 lg per fish and were challenged with betanodavirus by intramuscular injection. The vaccinated seabass was protected from nodaviral infection and 77.33% of relative percent survival (RPS) was recorded.

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Delivery of DNA vaccine using chitosan–tripolyphosphate (CS/TPP) nanoparticles in Asian sea bass, Lates calcarifer (Bloch, 1790) for protection against nodavirus infection

Cited by 55 publications

References 37 publications

Betanodavirus and VER Disease: A 30-year Research Review

Betanodavirus and VER Disease: A 30-year Research Review

Comparison of ELISA formats for detection of antibodies specific for nervous necrosis virus (Betanodavirus) in the serum of immunized barramundi Lates calcarifer and Australian bass Macquaria novemaculeata

Development, distribution and expression of a DNA vaccine against nodavirus in Asian Seabass,Lates calcarifier(Bloch, 1790)

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