Delineation of the healthy rabbit liver by immunohistochemistry – A technical note

Bürgisser, Gabriella Meier; Evrova, Olivera; Heuberger, Dorothea M.; Rieber, Julia; Giovanoli, Pietro; Calcagni, Maurizio; Buschmann, Johanna

doi:10.1016/j.acthis.2021.151795

Cited by 4 publications

(2 citation statements)

References 27 publications

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“…The slides were dehydrated, making them transparent, air-dried, sealed, and placed under a bright field microscope to interpret results. 25 …”

Section: Dosing Methodsmentioning

confidence: 99%

Effects of Jianpi Huayu Decoction on Th1/Th2 Immune Balance in Mice With Liver Cancer-Related Fatigue via the IL- 27/STAT1 Signaling Pathway

Jiayi,

Siru,

Xiaoqi

et al. 2024

Integr Cancer Ther

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Objective: The Chinese medicine Jianpi-Huayu decoction (健脾化瘀方, JPHY) can alleviate cancer-related fatigue in patients with liver cancer. However, its mechanism remains unclear. In this study, we used BALB/c mice with liver cancer model to investigate whether JPHY alleviates cancer-related fatigue by regulating Th1/Th2 immune balance; and the possible association with the IL-27/STAT1 signaling pathway. Methods: We established a mouse model of liver cancer fatigue. Mice were gavaged with physiological saline, low, medium, or high concentrations of JPHY respectively; and intraperitoneal injection of fludarabine (STAT1 pathway inhibitor) with JPHY for 21 days. We recorded the general condition of the mice, and assessed fatigue using scoring criteria and Exhausted Swimming Test. We calculated the spleen and thymus indices, performed H&E staining and immunohistochemical analysis on liver tumor tissues to observe the tumor proliferation marker ki67. We quantified the secretion levels of IFN-γ and IL-2 produced by Th1 cells in serum and splenic lymphocytes, as well as the secretion of IL-4, IL-10 by Th2 cells, and IL-27 in the signaling pathway through ELISA analysis. We evaluated the expression levels of p-STAT1 and STAT1 in spleen tissues using Western blot analysis. Results: JPHY exhibits a therapeutic effect on hepatocellular carcinoma-induced splenomegaly in murine models by upregulating the pro-inflammatory cytokines IFN-γ and IL-2 and downregulating the anti-inflammatory cytokines IL-4 and IL-10. Moreover, JPHY suppresses ki67 expression, reduces tumor-related inflammation infiltration, and ameliorates cancer-associated fatigue. Additionally, the expression of phosphorylated protein p-STAT1 is down-regulated. Conclusion: JPHY may improve the Th1/Th2 immune balance through its anti-inflammatory effects and promotion of IL-27-induced STAT1 phosphorylation, thereby alleviating fatigue in mice with liver cancer.

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“…The slides were dehydrated, making them transparent, air-dried, sealed, and placed under a bright field microscope to interpret results. 25 …”

Section: Dosing Methodsmentioning

confidence: 99%

Effects of Jianpi Huayu Decoction on Th1/Th2 Immune Balance in Mice With Liver Cancer-Related Fatigue via the IL- 27/STAT1 Signaling Pathway

Jiayi,

Siru,

Xiaoqi

et al. 2024

Integr Cancer Ther

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“…In modern human medicine, the New Zealand white rabbit is an appropriate animal model for anatomical and histological studies because it is calm and tolerant of disorders better than other species [8]. Besides this, nephrectomy is most commonly performed on healthy rabbits for research purposes because it serves as a model for human renal damage and kidney transplantation [9], so a rabbit kidney was used for this study because several important parameters can be evaluated, where tissue morphology and composition can be studied qualitatively and quantitatively [10]. After nephrectomy, the process of compensation by the remaining kidney leads to structural hypertrophy and functional hyperfiltration in the glomeruli, regarding compensatory structural adaptations, some studies have shown that the degree of structural hypertrophy in the remnant kidney is significantly correlated with postoperative and recovery of renal function [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Nephrectomy on Some Haemato-Biochemical Parameters in Adult White Male New Zealand Rabbits Strain

Ali,

Al-Kattan

2025

Egyptian Journal of Veterinary Sciences

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HE CURRENT STUDY aimed to evaluate the effect of removing the left kidney on renal functions and some hematological variables in white male New Zealand rabbits. This study enrolled randomly on 60 rabbits which allocated in four equal groups 15 of each. Total nephrectomy was performed aseptically for all rabbits and then general health, clinical, and biochemical hematological investigations were taken one month after nephrectomy, then two months, and three months later. The results showed a significant decrease in the number of red blood cells, hemoglobin concentration, white blood cells, and Packed Cells Volume P.C.V. % one month after the operation, then these variables began to rise after two and then three months, compared with the control group, and at the level of probability (P≤0.05). While biochemical analysis for kidney function showed a significant increase in the concentrations of uric acid, urea, and creatinine, in the group of rabbits one month after the nephrectomy compared to the control group, while the level of these variables began to decrease after two months, up to the third month, but this decline did not reach the level of the control group, and at the level of probability (P≤0.05). Conclusion: in this research, it has been found that the unilateral nephrectomy in white male New Zealand rabbits leads to physiological and biochemical changes that vary between decrease and increase depending on over different time periods one, tow, and three months after total nephrectomy.

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Liver organoids cocultured on decellularized native liver scaffolds as a bridging therapy improves survival from liver failure in rabbits

Septiana,

Ayudyasari,

Gunardi

et al. 2023

In Vitro Cell.Dev.Biol.-Animal

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Delineation of the healthy rabbit liver by immunohistochemistry – A technical note

Cited by 4 publications

References 27 publications

Effects of Jianpi Huayu Decoction on Th1/Th2 Immune Balance in Mice With Liver Cancer-Related Fatigue via the IL- 27/STAT1 Signaling Pathway

Effects of Jianpi Huayu Decoction on Th1/Th2 Immune Balance in Mice With Liver Cancer-Related Fatigue via the IL- 27/STAT1 Signaling Pathway

Effect of Nephrectomy on Some Haemato-Biochemical Parameters in Adult White Male New Zealand Rabbits Strain

Liver organoids cocultured on decellularized native liver scaffolds as a bridging therapy improves survival from liver failure in rabbits

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