Delineation of the CYP505E subfamily of fungal self-sufficient in-chain hydroxylating cytochrome P450 monooxygenases

Smit, Martha S.; Maseme, Mpeyake Jacob; Marwijk, Jacqueline van; Aschenbrenner, Jasmin C; Opperman, Diederik J.

doi:10.1007/s00253-022-12329-8

Cited by 4 publications

(2 citation statements)

References 40 publications

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“…A total of 29 distinct compounds were present in F2. Dodecanol and dodecanoic acid were found in the F1 variety and discovered to be hydroxylated at the ω-7 position to form δ -dodecalactone by sub-terminal fatty acid hydroxylases, which is crucial for future research on cultivating and transforming the aroma among different cultivars of F. filiformis [ 32 ]. F3 comprised 15 distinctive compounds.…”

Section: Resultsmentioning

confidence: 99%

Variations in Key Aroma Compounds and Aroma Profiles in Yellow and White Cultivars of Flammulina filiformis Based on Gas Chromatography–Mass Spectrometry–Olfactometry, Aroma Recombination, and Omission Experiments Coupled with Odor Threshold Concentrations

Song,

Sun,

et al. 2024

Foods

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Flammulina filiformis (F. filiformis) is called the ‘benefiting intelligence’ mushroom. There is a notable difference between a yellow cultivar (with a robust aroma) and a white mutant cultivar (with a high yield) of F. filiformis. A thorough analysis of aroma differences is essential to improve the aroma of high-yield strains. This study employed a combination of gas chromatography–mass spectrometry–olfactometry (GC-MS-O) and aroma extract dilution analysis (AEDA) to analyze the variations in aroma compounds. Then, the contribution of the odorants was determined using flavor dilution (FD) factors and odor activity values (OAVs). Aroma omission and recombination experiments were used to identify the key odorants. A total of 16 key aroma compounds were characterized in F. filiformis, along with four eight-carbon volatiles (3-octanone, 3-octanol, octanal, and 1-octen-3-ol). Finally, the dominant aroma characteristic was “sweet” for the yellow strain, while it was “green” for the white strain. More research is required to investigate the enzymes and corresponding genes that regulate the synthesis of aroma compounds in F. filiformis for future breeding programs.

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Section: Resultsmentioning

confidence: 99%

Variations in Key Aroma Compounds and Aroma Profiles in Yellow and White Cultivars of Flammulina filiformis Based on Gas Chromatography–Mass Spectrometry–Olfactometry, Aroma Recombination, and Omission Experiments Coupled with Odor Threshold Concentrations

Song,

Sun,

et al. 2024

Foods

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“…We, and others, have explored CYPs for the selective in-chain hydroxylation of saturated fatty acids for the production of δ-lactones. CYP505E3 hydroxylates dodecanoic acid or dodecanol at ω-7 (C5) for the production of δ-dodecalactone, , and CYP116B46 has been shown to hydroxylate both decanoic and dodecanoic acid at C5 to form the corresponding δ-lactones . To date, only the lactonization of methyl hexanoate has been achieved with a UPO, through typical subterminal (ω-1) hydroxylation .…”

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confidence: 99%

Lactones from Unspecific Peroxygenase-Catalyzed In-Chain Hydroxylation of Saturated Fatty Acids

Ebrecht,

Mofokeng,

Hollmann

et al. 2023

Org. Lett.

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γand δ-lactones are valuable flavor and fragrance compounds. Their synthesis depends on the availability of suitable hydroxy fatty acid precursors. Three short unspecific peroxygenases were identified that selectively hydroxylate the C4 and C5 positions of C8−C12 fatty acids to yield after lactonization the corresponding γand δ-lactones. A preference for C4 over C5 hydroxylation gave γ-lactones as the major products. Overoxidation of the hydroxy fatty acids was addressed via the reduction of the resulting oxo acids using an alcohol dehydrogenase in a bienzymatic cascade reaction.

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Cytochrome P450 for environmental remediation: catalytic mechanism, engineering strategies and future prospects

Yang,

Yu,

Qian

et al. 2023

World J Microbiol Biotechnol

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Delineation of the CYP505E subfamily of fungal self-sufficient in-chain hydroxylating cytochrome P450 monooxygenases

Cited by 4 publications

References 40 publications

Variations in Key Aroma Compounds and Aroma Profiles in Yellow and White Cultivars of Flammulina filiformis Based on Gas Chromatography–Mass Spectrometry–Olfactometry, Aroma Recombination, and Omission Experiments Coupled with Odor Threshold Concentrations

Variations in Key Aroma Compounds and Aroma Profiles in Yellow and White Cultivars of Flammulina filiformis Based on Gas Chromatography–Mass Spectrometry–Olfactometry, Aroma Recombination, and Omission Experiments Coupled with Odor Threshold Concentrations

Lactones from Unspecific Peroxygenase-Catalyzed In-Chain Hydroxylation of Saturated Fatty Acids

Cytochrome P450 for environmental remediation: catalytic mechanism, engineering strategies and future prospects

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