Delineation by use of specific monoclonal antibodies of the T-cell receptor and major histocompatibility complex interaction sites on the superantigen toxic shock syndrome toxin 1

Shimonkevitz, Richard; Boen, E; Malmström, Sebastian; Brown, Emma; Hurley, J M; Kotzin, Brian L.; Matsumura, Masazumi

doi:10.1128/iai.64.4.1133-1139.1996

Cited by 10 publications

(4 citation statements)

References 20 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The mouse hybridoma producing the anti-idiotypic monoclonal IgG1 anti(anti-K13) (clone 5868C [10]) was kindly provided by Dr K. Stein, NIH, Bethesda, MD. The control clone (IgG1 anti-toxic shock syndrome toxin-anti-TSST, clone 49A3.7 [12]), was kindly provided by Dr A. Tarkowski, Göteborg.…”

Section: Methodsmentioning

confidence: 99%

Antibodies Given Orally in the Neonatal Period Can Affect the Immune Response for Two Generations: Evidence for Active Maternal Influence on the Newborn's Immune System

Lundin¹,

Dahlman‐Höglund²,

Pettersson³

et al. 1999

Scand J Immunol

View full text Add to dashboard Cite

Two day old Wistar rats were tube fed with 1 or 10 micrograms of a mouse IgG1 monoclonal anti-idiotypic (a-Id) antibody that was directed against an anti-Escherichia coli-K13 capsular polysaccharide antibody. A control group was given 10 micrograms of an unrelated control antibody. Six weeks after the administration of antibodies, the rats were intestinally colonised with an ovalbumin (OVA)-producing E. coli O6K13 strain. At 8 weeks of age, the male rats (first generation) and the offsprings of the female rats (second generation), were parenterally immunised with OVA and dead wild type E. coli O6K13, and the immune response was followed. In the rats of the first generation, there were no major differences between the groups in the immune response to the bacterium. However, the offspring of the neonatally a-Id administered rats had a profoundly affected immune response to the idiotypically connected antigen K13, but also to other antigens on the bacteria. Thus, a-Id treatment in the first generation gave, in the second generation, a greatly enhanced serum antibody response to the spatially related antigens OVA and O6 LPS, as well as to the idiotypically connected antigen K13. Concurrently, the in vitro spleen cell proliferative response to both OVA and the wild type bacterium was lowered. Overall, greater effects were seen with the higher dose of a-Id. In conclusion, our results demonstrate that by giving monoclonal antibodies idiotypically connected to a single bacterial component to neonatal rats, one profoundly influence the immune response also to other-spatially related-bacterial antigens in their offsprings.

show abstract

Section: Methodsmentioning

confidence: 99%

Antibodies Given Orally in the Neonatal Period Can Affect the Immune Response for Two Generations: Evidence for Active Maternal Influence on the Newborn's Immune System

Lundin¹,

Dahlman‐Höglund²,

Pettersson³

et al. 1999

Scand J Immunol

View full text Add to dashboard Cite

show abstract

“…In this study, three anti-SEB mAbs (B327, 2B33, and B344; reference 23 ), two anti-SEA mAbs (A116.3 and A108.6; Kappler, J., and P. Marrack, unpublished data), and two anti– TSST-1 mAbs (TS327H1.1 and 477L; reference 24 ) were used. The anti–TSST-1 mAbs were a gift of M. Matsumura (NeXstar Pharmaceuticals, Lakewood, CO).…”

Section: Methodsmentioning

confidence: 99%

Transcytosis of Staphylococcal Superantigen Toxins

Hamad

Marrack

Kappler

1997

The Journal of Experimental Medicine

124

View full text Add to dashboard Cite

Staphylococcus aureus produces a set of proteins (e.g., staphylococcal enterotoxin A [SEA], SEB, toxic shock syndrome toxin 1 [TSST-1]) which act both as superantigens (SAgs) and toxins. Although their mode of action as SAgs is well understood, little is known about how they enter the body via the intestine and cause food poisoning. To examine this problem we used an in vitro culture system to study the capacity of class II MHC-negative human intestinal epithelial cells (Caco-2) to transcytose several staphylococcal toxins. We found that Caco-2 cells are capable of dosedependent, facilitated transcytosis of SEB and TSST-1, but not SEA. We extended these studies in vivo in mice by showing that ingested SEB appears in the blood more efficiently than SEA. Our data suggest that these toxins can cross the epithelium in an immunologically intact form. These results may have important implications for the pathogenesis of food poisoning.

show abstract

“…The concentration of immunoglobulins was determined by the radial immunodiffusion method [7]. These MoAbs discriminate various binding sites of the TSST-1 molecule [8]. MAbA5 is directed at an MHC class II binding domain of human monocytes, and neutralizes TSST-1induced lethality in D-galactosamine-sensitized BALB/c mice [9].…”

Section: Monoclonal Antibodiesmentioning

confidence: 99%

“…In the next three experiments antibodies 93E1.2 and 769U1.1 were used alone or in combination and administered after bacterial injection. They were chosen because of their property to inhibit TSST-1-mediated T cell stimulation and to inhibit TSST-1 binding to HLA-DR, respectively [8]. The antibodies were administered 48 h after bacterial injection and then every second or every third day until the mice were killed.…”

Section: Impact Of Anti-tsst-1 Antibodies On Development Of Arthritismentioning

confidence: 99%

The effect of site-specific monoclonal antibodies directed to toxic shock syndrome toxin-1 in experimentalStaphylococcus aureusarthritis

Verdrengh

Kum

Chow

et al. 1999

Clinical and Experimental Immunology

View full text Add to dashboard Cite

Staphylococcus aureus produces a large number of potential virulence factors, among these the superantigen toxic shock syndrome toxin-1 (TSST-1). We have recently demonstrated that TSST-1 is involved in the pathogenesis of septic arthritis. Recent data show that the TSST-1 molecule is composed of two distinct domains, one proposed to interact with T cell receptor (TCR) and one with the MHC class II. The aim of this study was to assess if interaction between TSST-1-specific MoAbs directed to sites on the MHC and/or TCR Vbeta affects the development of experimental S. aureus-induced arthritis. For that purpose we used a panel of seven MoAbs, which were injected intraperitoneally before and after inoculation with a TSST-1-producing S. aureus strain. Administration of antibodies did not affect the development of arthritis, suggesting inefficacy of such a procedure in neutralization of exotoxin-mediated disease manifestations.

show abstract

Delineation by use of specific monoclonal antibodies of the T-cell receptor and major histocompatibility complex interaction sites on the superantigen toxic shock syndrome toxin 1

Cited by 10 publications

References 20 publications

Antibodies Given Orally in the Neonatal Period Can Affect the Immune Response for Two Generations: Evidence for Active Maternal Influence on the Newborn's Immune System

Antibodies Given Orally in the Neonatal Period Can Affect the Immune Response for Two Generations: Evidence for Active Maternal Influence on the Newborn's Immune System

Transcytosis of Staphylococcal Superantigen Toxins

The effect of site-specific monoclonal antibodies directed to toxic shock syndrome toxin-1 in experimentalStaphylococcus aureusarthritis

Contact Info

Product

Resources

About