Delineating the antigenotoxic and anticytotoxic potentials of 4-methylimidazole against ethyl methanesulfonate toxicity in bone marrow cell of swiss albino mice

Abstract

4-Methylimidazole (4-MEI) is mostly used in beverages and coloring food, dark beers and common brands of cola drinks, which may contain more than 100 μg of this compound per 12-ounce serving. This study was aimed to investigate the antigenotoxic and anticytotoxic effects of 4-MEI (100, 130 and 160 mg/kg) against ethyl methanesulfonate (240 mg/kg) using chromosome aberrations (CAs) and Mitotic index (MI) tests in bone marrow cells of Swiss Albino Mice at 12 h and 24 h treatment periods. So, the t-test was used for the statistical analysis. In this research, 4-MEI at all concentrations for 12 h treatment period reduced chromosomal aberrations and at 130 and 160 mg/kg concentrations for 24 h treatment period increased chromosomal aberrations induced by EMS (240 mg/kg), but th ese reductions and increases were not signifi cant. Also, intraperitoneal injection of 4-MEI at doses of 100, 130 and 160 mg/kg combined with EMS (240 mg/kg) showed that the mitotic index was decreased at 100 and 130 mg/kg for 12h and 130 mg/kg for 24 h treatment periods, when compared to positive sample (EMS), but did not show any statistically difference from the EMS treated group. It can be concluded that 4-MEI might not be antigenotoxic and protective effects in bone marrow cells of Swiss Albino Mice, because 4-MEI could not reduce the chromosomal aberrations induced by EMS (Tab. 2, Fig. 2, Ref. 36). Text in PDF www.elis.sk.

Caramel Dye IV Induces Oxidative Stress Damage In Liver And Kidney From Mice

“…In this sense, mice treated with 4-MEI at 100, 130 and 160 g/kg presented a higher percentage of chromosomal mutations after 12 hours and 24 hours after 4-MEI exposure, indicating that 4-MEI is genocytotoxic to mice 23 . Besides, studies evidenced increased neonatal mortality in rodents treated with C-IV, the formation of full moon neoplasms 6 , tissue pigmentation alteration, and changes in body weight, in urine and hematological examinations 10 .…”