Delimitation of the time of death by immunohistochemical detection of insulin in pancreatic β-cells

Wehner, Frank; Wehner, Heinz‐Dieter; Schieffer, Maria Christine; Subke, Jörg

doi:10.1016/s0379-0738(99)00124-3

Cited by 55 publications

(27 citation statements)

References 6 publications

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“…Cases with Stages 3 and 4 decay were not included in this analysis, as the correlation between burial time and degree of decay was obvious in Detection after 29 days in pancreatic b-cells [29] Each finding includes up to three intervals.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the correlation between time corpses spent in in-ground graves and findings at exhumation

Breitmeier

Graefe-Kirci

Albrecht

et al. 2005

Forensic Science International

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Section: Resultsmentioning

confidence: 99%

Evaluation of the correlation between time corpses spent in in-ground graves and findings at exhumation

Breitmeier

Graefe-Kirci

Albrecht

et al. 2005

Forensic Science International

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“…Finally, the background was stained with haematoxylin (Picture 1). For the morphometric semi-quantitative analysis of stained β-cells in pancreas of rats, the stained Langerhan's islets in 13 ocular fields per specimen were measured at 10× magnification (Wehner et al, 1999). Effect of intraperitoneally administration of coriander seeds ethanol extract at a dose of 250 mg/kg body weight on the number of active beta cells in streptozotocin-induced diabetic fasted rats 3 h after administration.…”

Section: Determination Of Insulin Releasing Activitymentioning

confidence: 99%

Effect of coriander seed (Coriandrum sativum L.) ethanol extract on insulin release from pancreatic beta cells in streptozotocin‐induced diabetic rats

Eidi

Saeidi

et al. 2008

Phytotherapy Research

109

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Coriander (Coriandrum sativum L.) is grown as a spice crop all over the world. The seeds have been used to treat indigestion, diabetes, rheumatism and pain in the joints. In the present study, an ethanol extract of the seeds was investigated for effects on insulin release from the pancreatic beta cells in streptozotocin-induced diabetic rats. Blood samples were drawn from the retro-orbital sinus before and 1.5, 3 and 5 h after administration of the seed extract. Serum glucose levels were determined by the glucose oxidase method. To determine the insulin releasing activity, after extract treatment the animals were anaesthetized by diethyl ether, the pancreas was excised, fixed in 10% formaldehyde and embedded in paraffin for sectioning. Pancreatic sections of 5 microm were processed for examination of insulin-releasing activity using an immunocytochemistry kit. The results showed that administration of the ethanol extract (200 and 250 mg/kg, i.p.) exhibited a significant reduction in serum glucose. Administration of streptozotocin decreased the number of beta cells with insulin secretory activity in comparison with intact rats, but treatment with the coriander seed extract (200 mg/kg) increased significantly the activity of the beta cells in comparison with the diabetic control rats. The extract decreased serum glucose in streptozotocin-induced diabetic rats and increased insulin release from the beta cells of the pancreas.

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“…Wehner et al [60][61][62] studied if a positive immunoreaction to various antigens like insulin, thyroglobulin or calcitonin is correlated with the time since death ( Table 6). The philosophy of these investigations is that with increasing postmortem interval the tertiary structure of the antigen undergoes postmortal changes and due to protein denaturation staining becomes negative.…”

Section: Immunohistochemical Detection Of Insulin Thyroglobulin and mentioning

confidence: 99%

Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?

Madea

2005

Forensic Science International

176

101

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Delimitation of the time of death by immunohistochemical detection of insulin in pancreatic β-cells

Cited by 55 publications

References 6 publications

Evaluation of the correlation between time corpses spent in in-ground graves and findings at exhumation

Evaluation of the correlation between time corpses spent in in-ground graves and findings at exhumation

Effect of coriander seed (Coriandrum sativum L.) ethanol extract on insulin release from pancreatic beta cells in streptozotocin‐induced diabetic rats

Is there recent progress in the estimation of the postmortem interval by means of thanatochemistry?

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