Delignification overmatches hemicellulose removal for improving hydrolysis of wheat straw using the enzyme cocktail from Aspergillus niger

Wang, Jiahong; Chen, Xuntong; Chio, Chonlong; Yang, Chun; Su, Erzheng; Jin, Yongcan; Cao, Fuliang; Qin, Wensheng

doi:10.1016/j.biortech.2018.12.029

Cited by 29 publications

(11 citation statements)

References 42 publications

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“…The results of ANOVA for this model are shown in Table . The squared multiple R is 0.849, which indicates that the model can explain the test results well . The P values of ‘regression’ and ‘lack of fit’ were 0.032 and 0.592, respectively, which indicated that the model is credible .…”

Section: Resultsmentioning

confidence: 89%

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

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A low‐cost recycling system for the production of pectinase was built to improve the yield and quality of Muzao date juice and to reduce production costs. An Aspergillus niger strain, Gyx086, was selected from 20 A. niger strains on potato agar plates containing Muzao date residues, and the strain was used to produce an enzyme cocktail with high pectinase activity using Muzao date residues as the medium. The high pectinase activity level was therefore used in the production of Muzao date juice. Under optimized enzymatic hydrolysis conditions, a date juice yield of 92.88% was obtained, which was 11% higher than the control, and was not significantly different from a commercial preparation. Further storage trials indicated that the clarity, viscosity, storage stability, and soluble solid content could also be greatly improved by enzymatic treatment. These results suggested that the cheap experimental preparation could effectively replace the commercial preparation used for the production of date juice. This is the first systematic report detailing the production of Muzao date juice using a cheap and effective pectinase cocktail, which could promote the development of the date juice industry. © 2019 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Resultsmentioning

confidence: 89%

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Yang

Wang

Chio

et al. 2019

Biofuels Bioprod Bioref

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“…Synergy between cellulolytic enzymes and these enzymes from Acremonium alcalophilum and Aspergillus nidulans has been described, which deserves more attention in the future [48,49]. The currently reported lignocellulosic degrading bacteria and fungi with industrial application prospects presented distinct optimal conditions for different enzymes even in the same induced enzyme system, and the stabilities against temperature and pH were also different, so it is necessary to determine the optimal conditions for enzymatic hydrolysis and saccharification with overall consideration [1,50,51]. In the present study, similar optimal temperature and pH for various functional enzymes in T. harzianum EM0925 enzyme cocktail were observed, which gave rise to better synergistic effects in efficient deconstruction of lignocellulose complex.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, bio-based industries have developed rapidly, and potential bio-based products including ethanol, aldehydes, organic acids, polyhydric alcohols, and other bio-chemicals and biomaterials have attracted a lot of attention. As the most important intermediate compounds of biological and chemical transformation from biomass, fermentable sugars (mainly glucose and xylose) are crucial for the production of downstream products [1,2]. Due to the structural complexity of lignocellulose, complete enzymatic deconstruction requires the synergistic action of cellulases, hemicellulases, and ligninases [3].…”

Section: Introductionmentioning

confidence: 99%

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Zhang

Yang

Luo

et al. 2020

IJMS

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Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications.

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“…The enzyme cocktail produced by A. oryzae showed the best enzymatic activity for endoxylanases (Table 1), which are responsible for hemicellulose hydrolysis. Hemicellulose is the most abundant part of corn cobs [35,36]. Moreover, cellulase and xyloglucanase activities were also found, suggesting an efficient biomass digestion.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Optimized Bioproduction of Itaconic and Fumaric Acids Based on Solid-State Fermentation of Lignocellulosic Biomass

et al. 2020

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The bioproduction of high-value chemicals such as itaconic and fumaric acids (IA and FA, respectively) from renewable resources via solid-state fermentation (SSF) represents an alternative to the current bioprocesses of submerged fermentation using refined sugars. Both acids are excellent platform chemicals with a wide range of applications in different market, such as plastics, coating, or cosmetics. The use of lignocellulosic biomass instead of food resources (starch or grains) in the frame of a sustainable development for IA and FA bioproduction is of prime importance. Filamentous fungi, especially belonging to the Aspergillus genus, have shown a great capacity to produce these organic dicarboxylic acids. This study attempts to develop and optimize the SSF conditions with lignocellulosic biomasses using A. terreus and A. oryzae to produce IA and FA. First, a kinetic study of SSF was performed with non-food resources (wheat bran and corn cobs) and a panel of pH and moisture conditions was studied during fermentation. Next, a new process using an enzymatic cocktail simultaneously with SSF was investigated in order to facilitate the use of the biomass as microbial substrate. Finally, a large-scale fermentation process was developed for SSF using corn cobs with A. oryzae; this specific condition showed the best yield in acid production. The yields achieved were 0.05 mg of IA and 0.16 mg of FA per gram of biomass after 48 h. These values currently represent the highest reported productions for SSF from raw lignocellulosic biomass.

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Delignification overmatches hemicellulose removal for improving hydrolysis of wheat straw using the enzyme cocktail from Aspergillus niger

Cited by 29 publications

References 42 publications

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Low‐cost recycling production of pectinase to increase the yield and quality of Muzao jujube juice by Aspergillus niger

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Optimized Bioproduction of Itaconic and Fumaric Acids Based on Solid-State Fermentation of Lignocellulosic Biomass

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