"Delfia" and "Amerlite": two sensitive nonisotopic immunoassay systems for assay of thyrotropin compared.

Parnham, A.J.; Tarbit, I

doi:10.1093/clinchem/33.8.1421

Cited by 19 publications

(1 citation statement)

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“…After the very early recognition of the value of rare earth biolabel complexes ( Soini and Hemmilä, 1979 ) and the proof of principle of the dissociation enhanced fluorescence immuno assay (DELFIA) ( Siitari et al, 1983 ) for antigens, the method was rapidly extended to the analysis of proteins and antibodies, enzymes, polypeptides, DNA, hormones, drugs and to FRETs and more ( Bailey et al, 1984 ; Parnham and Tarbit, 1987 ; Christopoulos and Diamandis, 1992 ; Selvin, 2002 ; Matsumoto et al, 2020 ). Time resolved luminescence imaging and time resolved flow cytometry ( Jin et al, 2009 ; Chen et al, 2020 ) are further methods, which have of recent drawn attention.…”

Section: Introductionmentioning

confidence: 99%

High brightness red emitting polymer beads for immunoassays: Comparison between trifluoroacetylacetonates of Europium

et al. 2023

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Efficiently luminescing spherical polymer particles (beads) in the nanoscale regime of up to approximately 250 nm have become very valuable tools in bioanalytical assays. Eu3+- complexes imbedded in polymethacrylate and polystyrene in particular proved to be extraordinarily useful in sensitive immunochemical and multi-analyte assays, and histo- and cytochemistry. Their obvious advantages derive from both, the possibility to realize very high ratios of emitter complexes to target molecules, and the intrinsically long decay times of the Eu3+-complexes, which allows an almost complete discrimination against bothersome autofluorescence via time-gated measuring techniques; the narrow line emission in conjunction with large apparent Stokes shifts are additional benefits with regard to spectral separation of excitation and emission with optical filters. Last but not least, a reasonable strategy to couple the beads to the analytes is mandatory. We have thus screened a variety of complexes and ancillary ligands; the four most promising candidates evaluated and compared to each other were β-diketonates (trifluoroacetylacetonates, R-CO-CH-CO-CF3, R = - thienyl, -phenyl, -naphthyl and -phenanthryl); highest solubilities in polystyrene were obtained with trioctylphosphine co-ligands. All beads had overall quantum yields in excess of 80% as dried powders and lifetimes well beyond 600 µs. Core-shell particles were devised for the conjugation to model proteins (Avidine, Neutravidine). Their applicability was tested in biotinylated titer plates using time gated measurements and a Lateral Flow Assay as practical examples.

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