Deletions within the amino-terminal half of the c-src gene product that alter the functional activity of the protein.

Abstract

To examine how amino acid sequences outside of the catalytic domain of pp6O-src influence the functional activity of this protein, we have introduced deletion mutations within the amino-terminal half of pp60cs. These mutations caused distinct changes in the biochemical properties of the c-src gene products and in the properties of cells infected with retroviruses carrying these mutant c-src genes. Cells expressing the c-srcNX protein, which contains a deletion of amino acids 15 to 89, displayed a refractile, spindle-shaped morphology, formed intermediate-sized, tightly packed colonies in soft agar, and contained elevated levels of cellular phosphotyrosine-containing proteins. Thus, deletion of amino acids 15 to 89 can activate the kinase activity and transforming potential of the c-src gene product. Deletion of amino acids 112 to 225, however, did not increase the kinase activity or transforming ability of pp60csrc; indeed, deletion of these sequences in c-srcHP suppressed phenotypic alterations induced by pp60.csrc* Cells expressing the c-srcNP or c-srcBS gene products (containing deletions of amino acids 15 to 225 and 55 to 169, respectively) displayed a fusiform, refractile morphology and formed diffuse colonies in soft agar; the mutant proteins displayed an increased in vitro protein-tyrosine kinase activity. However, only a few cellular proteins contained elevated levels of phosphotyrosine in vivo. Thus, deletions downstream of amino acid 89 severely restricted the ability of c-src to phosphorylate cellular substrates in vivo without affecting the intrinsic tyrosine kinase activity of the c-src gene product. These results suggest the existence of at least two modulatory regions within the amino-terminal half of pp6Ocsrc that are important for the regulation of tyrosine kinase activity and for the interaction of pp60"csrc with cellular substrates.Rous sarcoma virus (RSV) encodes a 60-kilodalton (kDa) tyrosine protein kinase, pp6Ov-src, that is both necessary and sufficient for the induction of oncogenic transformation by RSV (for reviews, see references 2 and 3). This protein represents a mutated variant of the normal cell protein, pp60csr, which is unable to induce oncogenic transformation even when expressed at high levels (for a review, see reference 26). There are multiple amino acid differences that distinguish the viral and cellular src gene products (60). Whereas many of the mutations in pp6Ov-sr' contribute to the transforming potential of this protein, it has been shown that single-amino-acid substitutions can activate the transforming potential of pp60c-src (26). Thus, the c-src gene product is highly sensitive to alterations in structure that affect the functional activity of this protein.pp6Osrc can be divided into distinct functional domains. The catalytic domain is contained within the carboxy-terminal half of the protein (5, 39). The greatest degree of amino acid sequence homology among all cellular and viral tyrosine kinases is found within this region (for a review, see reference 27). Muta...

DNMT3A and DNMT3B mediate autocrine hGH repression of plakoglobin gene transcription and consequent phenotypic conversion of mammary carcinoma cells

“…The PLA-KOGLOBIN promoter pGL-Pg5 (À297/ þ 61) was a gift from Dr Brabant (Potter et al, 2001). The dominant negative c-Src was a gift from Dr Brugge (Nemeth et al, 1989) and JAK2 was a gift from Dr Silvennoinen (Saharinen et al, 2000).…”

pp60v-src transformation of rat cells but not chicken cells strongly correlates with low-affinity phosphopeptide binding by the SH2 domain.

“…Additionally, this domain can serve a direct negative regulatory function. For example, in pp60csrc, which does not have transforming activity or high kinase activity (Parker et al, 1984), the SH2 domain negatively regulates kinase activity and transformation (Nemeth et al, 1989;Hirai and Varmus, 1990a;O'Brien et al, 1990;Seidel-Dugan et al, 1992) by binding to the P-Tyr at position 527 and stabilizing an inactive conformation (Liu et al, 1993b; reviewed in Cooper and Howell, 1993). The loss of Tyr-527 in pp6Ov-src is partly responsible for its transforming ability (Cartwright et al, 1987;Kmiecik and Shalloway, 1987;Piwnica-Worms et al, 1987; reviewed in Sefton and Hunter, 1986).…”

Biological and biochemical properties of the c-src+ gene product overexpressed in chicken embryo fibroblasts.

“…Total cell lysates were prepared and analyzed for phosphotyrosinecontaining proteins according to a previously published procedure (40). SB23 antibody was prepared by using phosphotyrosine (Sigma) conjugated to Keyhole limpet hemocyanin as the immunogen and was purified on a phosphotyramine-Sepharose column prepared with CNBr-activated Sepharose (Pharmacia, Inc., Piscataway, N.J.) as previously described (16 (Fig.…”