Deletions of the cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) genes, involved in the control of hydrogen sulfide biosynthesis, significantly affect lifespan and fitness components of Drosophila melanogaster

Shaposhnikov, Mikhail; Zakluta, Alexey S.; Zemskaya, Nadezhda; Guvatova, Zulfiya G.; Shilova, Victoria; Yakovleva, Daria; Gorbunova, Anastasia A.; Koval, Liubov; Ulyasheva, Natalia S.; Евгеньев, М. Б.; Zatsepina, Olga G.; Moskalev, Alexey

doi:10.1016/j.mad.2022.111656

Cited by 12 publications

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“…In the experiments described herein, we made use of several D. melanogaster strains obtained using the CRISPR/Cas9 technique. These strains with deletions of the three genes ( CBS, CSE, and MST ) were used and described in detail in our previous studies [ 23 , 28 ]. Transcriptomic studies demonstrated that the deletions of both CBS and CSE ( CBS-/- ; CSE-/- ) have a cumulative effect and drastically alter genome expression, with a more pronounced impact exerted by the deletion of the CBS gene.…”

Section: Introductionmentioning

confidence: 99%

Genes Responsible for H2S Production and Metabolism Are Involved in Learning and Memory in Drosophila melanogaster

et al. 2022

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The gasotransmitter hydrogen sulfide (H2S) produced by the transsulfuration pathway (TSP) is an important biological mediator, involved in many physiological and pathological processes in multiple higher organisms, including humans. Cystathionine-β-synthase (CBS) and cystathionine-γ-lyase (CSE) enzymes play a central role in H2S production and metabolism. Here, we investigated the role of H2S in learning and memory processes by exploring several Drosophila melanogaster strains with single and double deletions of CBS and CSE developed by the CRISPR/Cas9 technique. We monitored the learning and memory parameters of these strains using the mating rejection courtship paradigm and demonstrated that the deletion of the CBS gene, which is expressed predominantly in the central nervous system, and double deletions completely block short- and long-term memory formation in fruit flies. On the other hand, the flies with CSE deletion preserve short- and long-term memory but fail to exhibit long-term memory retention. Transcriptome profiling of the heads of the males from the strains with deletions in Gene Ontology terms revealed a strong down-regulation of many genes involved in learning and memory, reproductive behavior, cognition, and the oxidation–reduction process in all strains with CBS deletion, indicating an important role of the hydrogen sulfide production in these vital processes.

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Section: Introductionmentioning

confidence: 99%