Deletion of the Sphingosine Kinase-1 gene influences cell fate during hypoxia and glucose deprivation in adult mouse cardiomyocytes

Tao, Rong; Zhang, Jianqing; Vessey, Donald A.; Honbo, Norman; Karliner, Joel S.

doi:10.1016/j.cardiores.2007.01.015

Cited by 76 publications

(75 citation statements)

References 33 publications

(53 reference statements)

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“…Accumulating evidence has implicated the S1P metabolism in hypoxia in normal cells. Indeed, we and others have shown the involvement of SphK1 in the adaptation of cardiomyocytes to ischemia both in vitro and in animal models (11,12), and SphK1-null cardiomyocytes have been shown to be more susceptible to hypoxia (13). Moreover, increased proliferation of smooth muscle cells induced by hypoxia relies on the generation of S1P (14), and increased SphK1 expression was reported in pulmonary smooth muscle cells under both acute and chronic hypoxia (15).…”

Section: Introductionmentioning

confidence: 80%

When the Sphingosine Kinase 1/Sphingosine 1-Phosphate Pathway Meets Hypoxia Signaling: New Targets for Cancer Therapy

2009

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The reduction in the normal level of tissue oxygen tension or hypoxia is a characteristic of solid tumors that triggers the activation of signaling pathways promoting neovascularization, metastasis, increased tumor growth, and resistance to treatments. The activation of the transcription factor hypoxiainducible factor 1A (HIF-1A) has been identified as the master mechanism of adaptation to hypoxia. In a recent study, we identified the sphingosine kinase 1/sphingosine 1-phosphate (SphK1/S1P) pathway, which elicits various cellular processes including cell proliferation, cell survival, or angiogenesis, as a new modulator of HIF-1A activity under hypoxic conditions. Here, we consider how the SphK1/S1P signaling pathway could represent a very important target for therapeutic intervention in cancer. [Cancer Res 2009;69(9):3723-6]

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Section: Introductionmentioning

confidence: 80%

When the Sphingosine Kinase 1/Sphingosine 1-Phosphate Pathway Meets Hypoxia Signaling: New Targets for Cancer Therapy

2009

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“…In culture, S1P reduced apoptosis in mesoangioblasts and in cardiac cells (Donati et al, 2007). Tao et al (2007) found that decreased S1P synthesis reduced cell survival. Neutralisation of S1P with a monoclonal antibody blocked in vivo angiogenesis induced by fibroblast growth factor and VEGF (Argraves et al, 2010).…”

Section: Discussionmentioning

confidence: 98%

Sphingosine-1-phosphate and ceramide are associated with health and atresia of bovine ovarian antral follicles

Hernández‐Coronado

Guzmán

Espinosa-Cervantes

et al. 2015

Animal

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The follicle destiny towards ovulation or atresia is multi-factorial in nature and involves outcries, paracrine and endocrine factors that promote cell proliferation and survival (development) or unchain apoptosis as part of the atresia process. In several types of cells, sphingosine-1-phospate (S1P) promotes cellular proliferation and survival, whereas ceramide (CER) triggers cell death, and the S1P/CER ratio may determine the fate of the cell. The aim of present study was to quantify S1P and CER concentrations and their ratio in bovine antral follicles of 8 to 17 mm classified as healthy and atretic antral follicles. Follicles were dissected from cow ovaries collected from a local abattoir. The theca cell layer, the granulosa cells and follicular fluid were separated, and 17β-estradiol (E2) and progesterone (P4) concentrations were measured in the follicular fluid by radioimmunoassay. Based on the E2/P4 ratio, the follicles were classified as healthy (2.2 ± 0.3) or atretic (0.2 ± 0.3). In both follicular compartments (granulosa and theca cell layer), sphingolipids were extracted and S1P and CER concentrations were quantified by HPLC (XTerra RP18; 5 µm, 3.0 × 150 mm column). Results showed that in both follicular compartments, S1P concentrations were higher in healthy antral follicles than in atretic antral follicles (P < 0.05). The concentration of CER in the granulosa cells was higher in atretic antral follicles than in healthy antral follicles, but no differences were observed in the theca cell layer. The S1P/CER ratio in both follicular compartments was also higher in healthy antral follicles. Interestingly, in these follicles, there was a 45-fold greater concentration of S1P than CER in the granulosa cells (P < 0.05), whereas in the theca cell layer, S1P had only a 14-fold greater concentration than CER when compared with atretic antral follicles. These results suggest that S1P plays a role in follicle health, increasing cellular proliferation and survival. In contrast, reduction of S1P and the S1P/CER in the antral follicle could trigger cellular death and atresia.Keywords: bovine, antral follicle, atresia, ceramide, sphingosine-1-phospohate Implications Follicular development involves selection of a follicle apt to grow in response to stimuli (FSH and LH) that induce proliferation and survival of follicular cells so that it reaches ovulation. However, if follicles do not receive these stimuli (reduction in FSH and LH or their receptors), cellular death signals are triggered and the follicle fate will be atresia. Sphingosine-1-phospohate (S1P) induces survival and proliferation of cells, whereas ceramide (CER) is involved in cellular death. The present study reveals that S1P and CER concentrations differ within follicular status suggesting these sphingolipids may participate in follicular health or atresia.Knowledge of signals that control follicular development and atresia may help us to improve follicular development and thus reproductive performance. IntroductionThe development of bovine ovarian follic...

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“…Additionally, a connection has been hypothesized between the SphK1/S1P metabolism and hypoxia based on the SphK1-dependent vascular smooth muscle cell growth increase under acute hypoxic stress (33) and the transcriptional mediated SphK1 expression in pulmonary smooth muscle cells under both acute and chronic hypoxia (34). More recently, SphK1-null cardiomyocytes have been shown to be more susceptible to hypoxia and glucose deprivation (35). A single article has recently suggested a link between SphK1 and CoCl 2 chemically induced hypoxia in cancer cells (36).…”

Section: Discussionmentioning

confidence: 99%

Sphingosine Kinase 1: A New Modulator of Hypoxia Inducible Factor 1α during Hypoxia in Human Cancer Cells

et al. 2008

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Here, we provide the first evidence that sphingosine kinase 1 (SphK1), an oncogenic lipid kinase balancing the intracellular level of key signaling sphingolipids, modulates the transcription factor hypoxia inducible factor 1A (HIF-1A), master regulator of hypoxia. SphK1 activity is stimulated under low oxygen conditions and regulated by reactive oxygen species. The SphK1-dependent stabilization of HIF-1A levels is mediated by the Akt/glycogen synthase kinase-3B signaling pathway that prevents its von Hippel-Lindau protein-mediated degradation by the proteasome. The pharmacologic and RNA silencing inhibition of SphK1 activity prevents the accumulation of HIF-1A and its transcriptional activity in several human cancer cell lineages (prostate, brain, breast, kidney, and lung), suggesting a canonical pathway. Therefore, we propose that SphK1 can act as a master regulator for hypoxia, giving support to its inhibition as a valid strategy to control tumor hypoxia and its molecular consequences. [Cancer Res 2008;68(20):8635-42]

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Deletion of the Sphingosine Kinase-1 gene influences cell fate during hypoxia and glucose deprivation in adult mouse cardiomyocytes

Abstract: As the effect of GM-1 is blocked both at the receptor and the G-protein (Gi) levels, we conclude that S1P generated by GM-1 treatment must be exported from the cell and acts in a paracrine or autocrine manner to couple with its cognate receptor.

Cited by 76 publications

References 33 publications

When the Sphingosine Kinase 1/Sphingosine 1-Phosphate Pathway Meets Hypoxia Signaling: New Targets for Cancer Therapy

When the Sphingosine Kinase 1/Sphingosine 1-Phosphate Pathway Meets Hypoxia Signaling: New Targets for Cancer Therapy

Sphingosine-1-phosphate and ceramide are associated with health and atresia of bovine ovarian antral follicles

Sphingosine Kinase 1: A New Modulator of Hypoxia Inducible Factor 1α during Hypoxia in Human Cancer Cells

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