Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of Listeria monocytogenes

Abstract

Background Listeria monocytogenes is a gram-positive bacterium that causes listeriosis mainly in immunocompromised hosts. It can also cause foodborne outbreaks and has the ability to adapt to various environments. Peptide uptake in gram-positive bacteria is enabled by oligopeptide permeases (Opp) in a process that depends on ATP hydrolysis by OppD and F. Previously a putative protein Lmo2193 was predicted to be OppD, but little is known about the role of OppD in major processes of L. monocytogenes , such as growth, virulence, and biofilm formation. Objectives To determine whether the virulence traits of L. monocytogenes are related to OppD. Methods In this study, lmo2193 gene deletion and complementation strains of L. monocytogenes were generated and compared with a wild-type strain for the following: adhesiveness, invasion ability, intracellular survival, proliferation, 50% lethal dose (LD 50 ) to mice, and the amount bacteria in the mouse liver, spleen, and brain. Results The results showed that virulence of the deletion strain was 1.34 and 0.5 orders of magnitude higher than that of the wild-type and complementation strains, respectively. The function of Lmo2193 was predicted and verified as OppD from the ATPase superfamily. Deletion of lmo2193 affected the normal growth of L. monocytogenes , reduced its virulence in cells and mice, and affected its ability to form biofilms. Conclusions Deletion of the oligopeptide transporter Lmo2193 decreases the virulence of L. monocytogenes . These effects may be related to OppD's function, which provides a new perspective on the regulation of oligopeptide transporters in L. monocytogenes .

Drug sensitivity and genome-wide analysis of two strains of Mycoplasma gallisepticum with different biofilm intensity

“…oppA and oppD belong to the ATP-dependent transporter family. They exert different roles in bacterial transport and have been shown to influence biofilm formation ( Lee et al, 2004 ; Li H. et al, 2020 ). PdhA , pdhB , pdhC , and pdhD were all predicted as genes encoding pyruvate dehydrogenase complex ( PDH ).…”

Transcriptome analysis of the biofilm formation mechanism of Vibrio parahaemolyticus under the sub-inhibitory concentrations of copper and carbenicillin

“…The quorum sensing system (QS) consists of signals and receptors, quorum sensing regulators, and other controlled genes promotes the production of bacterial extracellular proteins and regulates the biofilm. The genes oppA, oppB, oppC, oppD, and oppF belong to the same operon opp regulation that can regulate the production of bacterial oligopeptide osmotase (Opp) (Li et al, 2020). They were up-regulated by about 1.24, 2.05, 1.46, 1.47, and 0.85 folds under 1/2 MIC CARB treatment, and down-regulated by about 1-4 folds under 1/2 MIC Cu 2+ and 1/2 MIC Cu 2+ +CARB treatment (Figure 9B), which also corresponded to the amount of membrane production earlier.…”

Virulence of Listeria Monocytogenes in Mice is Enhanced by Deletion of Pathogenicity Island 4

“…According to the method of Li [40], HCMECs cells were cultured in a 12-well plate at 37 ℃ and 5% CO 2 .…”