Deletion of the murein hydrolase CbpD reduces transformation efficiency in Streptococcus thermophilus

Biørnstad, Truls Johan; Ohnstad, Hilde Solheim; Håvarstein, Leiv Sigve

doi:10.1099/mic.0.056150-0

Cited by 6 publications

(7 citation statements)

References 28 publications

(38 reference statements)

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“…Genes encoding these three CBPs were found in the S. oralis , S. mitis , and the S. infantis genomes, which contained the lic clusters described above. All streptococcal genomes that did not contain CBPs, and thus did not contain CbpD, encoded a protein related to LytF of S. gordonii and possessing a similar function ( 47 , 48 ) or another, new autolysin with a related CHAP domain ( Streptococcus sp. strains DD10 and DD13).…”

Section: Resultsmentioning

confidence: 99%

Highly Variable Streptococcus oralis Strains Are Common among Viridans Streptococci Isolated from Primates

Denapaite

Rieger

Köndgen

et al. 2016

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Streptococcus pneumoniae is a rare example of a human-pathogenic bacterium among viridans streptococci, which consist of commensal symbionts, such as the close relatives Streptococcus mitis and S. oralis. We have shown that S. oralis can frequently be isolated from primates and a variety of other viridans streptococci as well. Genes and genomic islands which are known pneumococcal virulence factors are present in S. oralis and S. mitis, documenting the widespread occurrence of these compounds, which encode surface and secreted proteins. The frequent occurrence of CRISP-Cas gene clusters and a surprising variation of a set of small noncoding RNAs are factors to be considered in future research to further our understanding of mechanisms involved in the genomic diversity driven by horizontal gene transfer among viridans streptococci.

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Section: Resultsmentioning

confidence: 99%

Highly Variable Streptococcus oralis Strains Are Common among Viridans Streptococci Isolated from Primates

Denapaite

Rieger

Köndgen

et al. 2016

mSphere

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“…These results clearly show that DNA uptake in S. pneumoniae and S. gordonii does not depend on CbpD-Sp or LytF and therefore argue strongly against a role for these murein hydrolases in assembly of the DNA uptake apparatus. In contrast, depending on the experimental setup used, deletion of the cbpD-St gene in S. thermophilus resulted in a 6-to 18-fold reduction in transformation efficiency (7). The presence of CbpD-St is not required for transformation, but it stimulates the process.…”

Section: Do Fratricins Have a Role In Dna Uptake?mentioning

confidence: 89%

“…Zymogram analyses in which S. thermophilus cells were incorporated in the resolving gel as the substrate revealed that CbpD-St is a murein hydrolase that is produced only during competence. Furthermore, examination of the cell surface-binding properties of a fusion protein in which the CHAP domain had been exchanged with green fluorescent protein (GFP) demonstrated that the C-terminal domain of CbpD-St specifically attaches this protein to the equatorial region of S. thermophilus cells (7). Evidently, the CbpD-like proteins depicted in Fig.…”

Section: Cbpd Proteins Without Cbdsmentioning

confidence: 99%

“…Experiments have shown that about 4% of the cells in a liquid culture of S. thermophilus are lysed when the culture is induced to competence by the addition of ComS*. CbpD-St is very sensitive to oxidative inactivation by H 2 O 2 , and it is therefore likely that the fraction of lysed cells would be higher in a microaerophilic or anaerobic environment (7).…”

Section: Are Fratricins Used For Acquisition Of Homologous Dna?mentioning

confidence: 99%

“…The latter possibility is supported by the findings that CbpD-Sp and CbpD-St display similar but nonidentical binding patterns. CbpD-Sp binds to the equator, septum, and poles of pneumococci, while CbpD-St binds predominantly to the equatorial region of S. thermophilus cells (7,22).…”

Section: Cbpd Proteins Without Cbdsmentioning

confidence: 99%

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Properties and Biological Role of Streptococcal Fratricins

Berg

Biørnstad

Johnsborg

et al. 2012

Appl Environ Microbiol

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ABSTRACTCompetence for natural genetic transformation is widespread in the genusStreptococcus. The current view is that all streptococcal species possess this property. In addition to the proteins required for DNA uptake and recombination, competent streptococci secrete muralytic enzymes termed fratricins. Since the synthesis and secretion of these cell wall-degrading enzymes are always coupled to competence development in streptococci, fratricins are believed to carry out an important function associated with natural transformation. This minireview summarizes what is known about the properties of fratricins and discusses their possible biological roles in streptococcal transformation.

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High‐efficiency transformation of Streptococcus thermophilus using electroporation

et al. 2021

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BACKGROUND: Streptococcus thermophilus, one of the most important lactic acid bacteria, is widely used in food fermentation, which is beneficial to improve food quality. However, the current genetic transformation systems are inefficient for S. thermophilus S-3, which hinders its further study. RESULTS: We developed three electroporation transformation methods for S. thermophilus S-3, and optimized various parameters to enhance the transformation efficiency up to 1.3 × 10 6 CFU/∼g DNA, which was 32-fold higher than that of unoptimized. Additionally, transcriptional analysis showed that a series of competence genes in S. thermophilus S-3 were remarkedly upregulated after optimization, indicating that improvement of transformation efficiency was attributed to the expression level of competence genes. Furthermore, to prove their potential, expression of competence genes (comEA, cbpD and comX) were employed to increase transformation efficiency. The maximum transformation efficiency was obtained by overexpression of comEA, which was 14-fold higher than that of control.CONCLUSION: This is the first report of competence gene expression for enhancing transformability in S. thermophilus, which exerts a positive effect on the development of desirable characteristics strains.

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Deletion of the murein hydrolase CbpD reduces transformation efficiency in Streptococcus thermophilus

Cited by 6 publications

References 28 publications

Highly Variable Streptococcus oralis Strains Are Common among Viridans Streptococci Isolated from Primates

Highly Variable Streptococcus oralis Strains Are Common among Viridans Streptococci Isolated from Primates

Properties and Biological Role of Streptococcal Fratricins

High‐efficiency transformation of Streptococcus thermophilus using electroporation

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