Deletion of miR-15a inhibited glioma development via targeting Smad7 and inhibiting EMT pathway

Guo, Yanfeng; Gao, Xiaopeng; An, Shien; Li, Xin; Pan, Lekun; Liu, Hongyan; Liu, Jixiang; Gao, Jianzhou; Zhao, Zhihuang; Li, Gang; Han, Yonggang; Li, Yabin; Ji, Zhisheng

doi:10.18632/aging.203684

Cited by 5 publications

(6 citation statements)

References 25 publications

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“…Anti-miRNA oligonucleotide (AMO)-mediated inhibition of miR-15a in SHG139 cells reduced migration and downregulated mesenchymal markers-Vimentin and N-cadherin. However, the combined knockdown of miR-15a and SMAD7 reversed these effects [79]. Further, miR-15a inhibition attenuated GBM tumour growth in vivo.…”

Section: Mir-15amentioning

confidence: 95%

“…Guo et al, using a microarray screen, observed that miR-15a-5p is upregulated in glioma tissues [79]. Bioinformatics analysis and luciferase reporter assay confirmed the direct interaction of miR-15a with SMAD7.…”

Section: Mir-15amentioning

confidence: 96%

“…Bioinformatics analysis and luciferase reporter assay confirmed the direct interaction of miR-15a with SMAD7. Overexpression of miR-15a promoted migration and invasion of SHG139 cells [79]. Whereas inhibition of miR-15a displayed the opposite effect, indicating the tumour-promoting ability of miR-15a [79].…”

Section: Mir-15amentioning

confidence: 96%

“…Overexpression of miR-15a promoted migration and invasion of SHG139 cells [79]. Whereas inhibition of miR-15a displayed the opposite effect, indicating the tumour-promoting ability of miR-15a [79]. Anti-miRNA oligonucleotide (AMO)-mediated inhibition of miR-15a in SHG139 cells reduced migration and downregulated mesenchymal markers-Vimentin and N-cadherin.…”

Section: Mir-15amentioning

confidence: 99%

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Role of Non-Coding RNAs in TGF-β Signalling in Glioma

Shree,

Sharma

2023

Brain Sciences

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Brain tumours and Gliomas, in particular, are among the primary causes of cancer mortality worldwide. Glioma diagnosis and therapy have not significantly improved despite decades of efforts. Autocrine TGF-β signalling promotes glioma proliferation, invasion, epithelial-to-mesenchymal transition (EMT), and drug resistance. Non-coding RNAs such as miRNA, lncRNA, and circRNAs have emerged as critical transcriptional and post-transcriptional regulators of TGF-β pathway components in glioma. Here, we summarize the complex regulatory network among regulatory ncRNAs and TGF-β pathway during Glioma pathogenesis and discuss their role as potential therapeutic targets for Gliomas.

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Section: Mir-15amentioning

confidence: 95%

Section: Mir-15amentioning

confidence: 96%

Section: Mir-15amentioning

confidence: 96%

Section: Mir-15amentioning

confidence: 99%

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Role of Non-Coding RNAs in TGF-β Signalling in Glioma

Shree,

Sharma

2023

Brain Sciences

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“…In the nucleus, SMAD7 disrupts the interaction between the SMAD2/3/4 complex and DNA 21 . Recent studies have shown that noncoding RNAs, including miRNAs and circRNAs, contribute to cancer progression by regulating SMAD7 and modulating TGF‐β signaling 22–25 . However, the mechanisms underlying abnormal SMAD7 expression and its role in GC progression remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA circWNK1 inhibits the progression of gastric cancer via regulating the miR‐21‐3p/SMAD7 axis

Dai,

Qiu,

Gao

et al. 2024

Cancer Science

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Gastric cancer (GC) is a highly aggressive malignancy with limited treatment options for advanced‐stage patients. Recent studies have highlighted the role of circular RNA (circRNA) as a novel regulator of cancer progression in various malignancies. However, the underlying mechanisms by which circRNA contributes to the development and progression of GC remain poorly understood. In this study, we utilized microarrays and real‐time quantitative polymerase chain reaction (qRT‐PCR) to identify and validate a downregulated circRNA, hsa_circ_0003251 (referred to as circWNK1), in paired GC and normal tissues. Through a series of in vitro and in vivo gain‐of‐function and loss‐of‐function assays, we demonstrated that circWNK1 exerts inhibitory effects on the proliferation, migration, invasion, and epithelial–mesenchymal transition (EMT) of GC cells. Additionally, we discovered that circWNK1 acts as a competitive endogenous RNA (ceRNA) for SMAD7 by sequestering miR‐21‐3p. Our findings were supported by comprehensive biological information analysis, as well as RNA pull‐down, luciferase reporter gene, and western blot assays. Notably, the downregulation of circWNK1 in GC cells resulted in reduced SMAD7 expression, subsequently activating the TGF‐β signaling pathway. Collectively, our study reveals that circWNK1 functions as a tumor suppressor in GC by regulating the miR‐21‐3p/SMAD7‐mediated TGF‐β signaling pathway. Furthermore, circWNK1 holds promise as a potential biomarker for the diagnosis and treatment of GC.

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