Deletion of Gcw13 represses autophagy in Pichia pastoris cells grown in methanol medium with sufficient amino acids

The Transcriptomic Mechanism of a Novel Autolysis Induced by a Recombinant Antibacterial Peptide from Chicken Expressed in Pichia pastoris

“…As a result, the cells from the autolysis group had relatively weak cell viability, and the initiation of autolysis, induced by the recombinant AMP, was improved. Zou et al found that autophagy was induced in P. pastoris when cells were grown in an amino acid-rich methanol medium, and that the autophagy may be due to a GPI-anchored protein (Gcw13)-dependent decrease in amino acid uptake during methanol adaptation [ 17 ]. The P. pastoris mutant with the two deleted catabolite repressor genes MIG1 and MIG2 realized the derepression of the alcohol oxidase (AOX) when grown in glycerol.…”

“…However, the cell growth is affected at this time and is closely related to autophagy [ 16 ]. When cells encountered hypoxia, a lack of nutrition, organelle damage, abnormal protein accumulation, an invasion of some microorganisms, or chemical treatments, autophagy helped cells survive adversity by degrading intracellular components and maintaining intracellular physiological balance [ 17 ]. When P. pastoris was cultured on the amino acid-rich methanol medium, autophagy was induced.…”

“…When P. pastoris was cultured on the amino acid-rich methanol medium, autophagy was induced. A transcriptomic analysis showed that the DEGs related to amino acid biosynthesis were up-regulated, and the autophagy induced in P. pastoris resulted in a Gcw13-dependent decrease in amino acid uptake, which played a role in the endocytosis of the amino acid permease Gap1 [ 17 ]. A methanol-induced high-level production of the Hepatitis B virus surface antigen (HBsAg) was obtained in P. pastoris , to which the physiological responses of P. pastoris were analyzed through proteome and ultrastructural cell morphology methods.…”