Deletion of Dictyostelium discoideum Sir2A impairs cell proliferation and inhibits autophagy

Lohia, Rakhee; Jain, Punita; Jain, Mukul; Mishra, Himanshu; Burma, Pradeep Kumar; Shrivastava, Anju; Saran, Shweta

doi:10.1007/s12038-018-9753-6

Cited by 3 publications

(4 citation statements)

References 30 publications

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“…It contains a wider variety of histone variants that are more similar to vertebrates than other simple eukaryotic model organisms 44 – 46 . The major PTMs on histones are also observed in this organism, including ADPr 45 – 49 . Importantly, Dictyostelium also contains single copy histone genes that are amenable to genetic manipulation, opening up the possibility to perform gene replacement and site-specific mutation strategies to assess the functional significance of histone PTMs 45 , 50 , 51 .…”

Section: Introductionmentioning

confidence: 77%

“…Although the ability to phosphorylate S-T sites may be context dependent, we predict this strategy will be applicable to other ADPr sites in histones and other targets for Ser-ADPr. The conservation of the major histone PTMs in Dictyostelium 45 – 49 , together with the ability to generate site-specific mutations in histones, makes this an attractive system to study these possibilities.…”

Section: Discussionmentioning

confidence: 99%

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Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones

et al. 2022

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Although serine ADP-ribosylation (Ser-ADPr) by Poly(ADP-ribose)-polymerases is a cornerstone of the DNA damage response, how this regulates DNA repair and genome stability is unknown. Here, we exploit the ability to manipulate histone genes in Dictyostelium to identify that ADPr of the histone variant H3b at S10 and S28 maintains genome stability by integrating double strand break (DSB) repair with mitotic entry. Given the critical requirement for mitotic H3S10/28 phosphorylation, we develop separation of function mutations that maintain S10 phosphorylation whilst disrupting ADPr. Mechanistically, this reveals a requirement for H3bS10/28 ADPr in non-homologous end-joining by recruiting Ku to DSBs. Moreover, this also identifies H3bS10/S28 ADPr is critical to prevent premature mitotic entry with unresolved DNA damage, thus maintaining genome stability. Together, these data demonstrate how serine ADPr of histones coordinates DNA repair with cell cycle progression to maintain genome stability.

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Section: Introductionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones

et al. 2022

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“…Antisense RNA probe (DIG labeled; Roche Diagnostics, India) was synthesized by in vitro transcription from T3 promoter (a run off template generated by digestion with Sal I) and sense probe with T7 RNA polymerase (template generated by digestion with Bam HI). The probes were further used for hybridization in multicellular structures developed according to the protocol of Lohia et al ().…”

Section: Methodsmentioning

confidence: 99%

“…The probes were further used for hybridization in multicellular structures developed according to the protocol of Lohia et al (2018).…”

Section: Analysis Of Spatiotemporal Messenger Rna (Mrna) Expression B...mentioning

confidence: 99%

Deletion of Htt cause alterations in cAMP signaling and spatial patterning in Dictyostelium discoideum

Bhadoriya

Jain

Kaicker

et al. 2019

Journal Cellular Physiology

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In the present study, we have analyzed in detail the functions of Htt during growth and development of the protist, Dictyostelium discoideum by creating mutants (both overexpressor and knockout). The mRNA was present at all stages of growth and development. Overexpression of htt did not show any major anomaly, while deletion resulted in delayed aggregation territory formation and showed asynchronous development especially after slug stage. The slugs formed by htt − cells showed aberration in anterior–posterior boundary, showing increased prestalk region. DdHtt regulates STAT transcription factors in the tip organizer region that help maintain patterning and culmination. In chimeras with the wild‐type, htt − cells preferentially localized to the tip of the slug and basal disc regions of the fruiting body showing prestalk/stalk bias, while the overexpressing cells majorly populated the prespore/spore region showing spore bias. These differences could be attributed to protein kinase A (PKA)‐regulated cyclic adenosine monophosphate (cAMP) signaling.

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Glimpses of Dictyostelid research in India

Begum¹,

Saran²

2020

Int. J. Dev. Biol.

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Simple organisms are preferred for understanding the molecular and cellular function(s) of complex processes. Dictyostelium discoideum is a lower eukaryote, a protist and a cellular slime mould, which has been in recent times used for various studies such as cell differentiation, development, cell death, stress responses etc. It is a soil amoeba (unicellular) that undertakes a remarkable, facultative shift to multicellularity when exposed to starvation and requires signal pathways that result in alteration of gene expression and finally show cell differentiation. The amoebae aggregate, differentiate and form fruiting bodies with two terminally differentiated cells: the dead stalk (non-viable) and dormant spores (viable). In India, starting from the isolation of Dictyostelium species to morphogenesis, cell signalling and social evolution has been studied with many more new research additions. Advances in molecular genetics make Dictyostelium an attractive model system to study cell biology, biochemistry, signal transduction and many more.

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Deletion of Dictyostelium discoideum Sir2A impairs cell proliferation and inhibits autophagy

Cited by 3 publications

References 30 publications

Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones

Linking DNA repair and cell cycle progression through serine ADP-ribosylation of histones

Deletion of Htt cause alterations in cAMP signaling and spatial patterning in Dictyostelium discoideum

Glimpses of Dictyostelid research in India

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