Deletion of chromosome 11 and of 14q sequences in neuroblastoma

Abstract

Restriction fragment length polymorphism (RFLP) analysis carried out on 45 primary neuroblastomas showed deletion of chromosome 11 sequences in 12 of 37 (32%) informative cases. Both 11p and 11q probes were informative in seven tumors; loss of all of chromosome 11, of only 11p sequences, and of only 11q sequences was observed in 4, 1, and 2 tumors, respectively. A cytogenetic abnormality involving translocation of chromosome arm 11q to chromosome arm 1p was observed in a primary tumor. Deletion of 14q was observed in 6 of 27 (22%) informative cases. Deletion of chromosome 11 but not 14q may correlate with regional and metastatic disease. These results suggest a possible role for sequences localized to chromosome 11 and to 14q in the development and/or progression of neuroblastoma.

Novel risk stratification of patients with neuroblastoma by genomic signature, which is independent of molecular signature

“…The frequently observed GGP tumors are as follows: GGP1a tumors with both 1p loss and MYCN amplification and GGP3s tumors with 11q loss but without MYCN amplification. The former may belong to a typical MYCN-amplified neuroblastoma (White et al, 1995) with a 5-year cumulative survival rate of 42% in our series, whereas the latter to the so-called intermediate type tumor (Srivatsan et al, 1993;Attiyeh et al, 2005) with the rate of 75%. In GGP tumors, it is obvious that MYCN amplification has the most powerful impact on the patient prognosis.…”

Localization of deletion to a 300 Kb interval of chromosome 11q13 in cervical cancer

“…Besides cervical cancer, LOH studies have implicated chromosome 11q13 in a number of human tumors including breast, head and neck, neuroblastoma and endocrine tumors (Srivatsan et al, 1993;Zhuang et al, 1995;Chakrabarti et al, 1998;Venugopalan et al, 1998;Tanaka et al, 1998). Recently, the minimal 11q13 deletion for a nasopharyngeal carcinoma tumor suppressor gene was localized to a 1.8 Mb interval that overlapped with the 300 kb deletion observed in cervical cancer (Cheng et al, 2002).…”

Genomic structure and mutational analysis of the human KIF1B gene which is homozygously deleted in neuroblastoma at chromosome 1p36.2

“…We cloned the human KIF1B-b gene from a region that was homozygously deleted in the NB cell line, NB-1. We physically mapped the KIF1B-b gene to 1p36.2, telomeric to D1S244 between WI-14873 and stSG 14169, within the commonly deleted region of NB (Schwab et al, 1996;Srivatsan et al, 1993;Maris and Matthay, 1999). Using two KIF1B-b polymorphic markers and two microsatellite markers in both sides of KIF1B-b, we found that the incidence of AI in the KIF1B-b gene was slightly higher than in the D1S244 and D1S1350 loci.…”

“…One of these is ampli®cation of the MYCN gene that is associated with a poor prognosis in NB (Seeger et al, 1985;Hayashi et al, 1989;Brodeur and Fong, 1989). Another is the loss of heterozygosity (LOH) at the 1p (Fong et al, , 1992Takita et al, 1995;Schwab et al, 1996), long arm of chromosome 11 (11q) (Srivatsan et al, 1993) and 14q (Srivatsan et al, 1993;Suzuki et al, 1989) in NB. In addition to those mentioned above, we recently found a LOH at 2q, 9p and 18q (Takita et al, 1995(Takita et al, , 1998.…”