Deleted in Colon Cancer Protein Expression in Colorectal Cancer Metastases: A Major Predictor of Survival in Patients With Unresectable Metastatic Disease Receiving Palliative Fluorouracil-Based Chemotherapy

Aschele, C.; Debernardis, Domizia; Lonardi, Sara; Bandelloni, Roberto; Casazza, Simona; Monfardini, S.; Gallo, Luigi

doi:10.1200/jco.2004.08.066

Cited by 21 publications

(16 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The gene DCC , which maps to 18q21, is recognised as a key player in colorectal carcinogenesis and is the primary candidate for the biological effect of chromosome 18q allelic imbalance. Patients with locally advanced stage II or stage III disease appear to demonstrate a significantly poorer prognosis with loss of 18q 55 – 58…”

Section: Molecular Prognostic Factorsmentioning

confidence: 99%

Prognostic and predictive factors in colorectal cancer

Zlobec

Lugli

2008

Postgraduate Medical Journal

159

131

View full text Add to dashboard Cite

Prognostication of newly diagnosed colorectal cancer (CRC) predominantly relies on stage as defined by the UICC-TNM and American Joint Committee on Cancer classifications. Tumour extent, lymph node status, tumour grade and the assessment of lymphatic and venous invasion are still the most important morphological prognostic factors. Evidence suggests that tumour budding and tumour border configuration are important, additional histological parameters but are not regarded as essential in prognosis. Although several molecular features, such as LOH18q and TP53 mutation analysis, have shown promising results in terms of their prognostic value, the American Society of Clinical Oncology Tumor Markers Expert Panel does not currently recommend their use in routine practice. cDNA-microarray, PCR and fluorescence in situ hybridisation are now frequently used to identity potential prognostic indicators in CRC, but the applicability of these methods in routine use is likely to have limited impact. Reliable prognostic markers identified by immunohistochemical protein profiling have yet to be established. Randomisation of data sets, assessment of interobserver variability for protein markers and scoring systems, as well as the use of receiver operating characteristic curve analysis in combination with multimarker-phenotype analysis of several different markers may be an effective tactical approach to increase the value of immunohistochemical findings. This article reviews the well established and additional prognostic factors in CRC and explores the contribution of molecular studies to the prognostication of patients with this disease. Additionally, an approach to improve the prognostic value of immunohistochemical protein markers is proposed.

show abstract

Section: Molecular Prognostic Factorsmentioning

confidence: 99%

Prognostic and predictive factors in colorectal cancer

Zlobec

Lugli

2008

Postgraduate Medical Journal

159

131

View full text Add to dashboard Cite

show abstract

“…Various studies reported that DCC is a predictor of distant metastasis and prognosis in colorectal cancer (24)(25)(26). Furthermore, DCC expression has been shown recently to predict clinical outcome to fluorouracilbased chemotherapy (27). Other studies have reported that the DCC gene product induced apoptosis through a caspasedependent mechanism (28,29).…”

Section: Discussionmentioning

confidence: 99%

The gene expression profile represents the molecular nature of liver metastasis in colorectal cancer

Yamasaki

Takemasa²,

Komori³

et al. 2007

Int J Oncol

View full text Add to dashboard Cite

Abstract. The major cause of death in colorectal cancer is related to liver metastasis. Although the metastatic process has been well studied, many aspects of the molecular genetic basis of metastasis remain unclear. Elucidation of the molecular nature of liver metastasis is urgent to improve the outcome of colorectal cancer. We analyzed the chronological gene expression profiles of 104 colorectal samples corresponding to oncogenic development including normal mucosa, localized and metastasized primary tumors, and liver metastatic lesions as fundamental samples using a custom cDNA microarray. The gene expression patterns in 104 samples were classified into four groups closely associated with their metastatic status, and the genes of each group appropriately reflected the metastatic process. To investigate the existence of metastatic potential in primary tumors using metastasis-related genes detected by chronological analysis, we performed a hierarchical cluster and supervised classification analysis of 28 independent primary tumors. Hierarchical cluster analysis segregated the tumors according to their final metastatic status, rather than their clinical stages, and the profile of metastasized primary tumors resembled one of a metastatic lesion apart from a primary lesion rather than one of a non-metastasized primary tumor. Using the supervised classification approach, the expression profile of these genes allowed the classification of tumors diagnosed as localized cancer into two classes, the localized and the metastasized class, according to their final metastatic status. The disease-free survival and overall survival were significantly longer in the localized class than the metastasized class. Chronological analysis of the gene expression profile provides a better understanding of the metastatic process. Our results suggest that the metastatic potential is already encoded in the primary tumor and is detectable by a gene expression profile, which allows the prediction of liver metastasis in patients diagnosed with localized tumors and also the design of new strategies for the treatment and diagnosis of colorectal cancer.

show abstract

“…Pada tahun 1990, Fearon dan Vogelstein, seperti dikutip oleh Kelli 2 yang sudah memperkenalkan patogenesis karsinoma kolorektal dan dinyatakan bahwa paling tidak ditemukan 5 mutasi gen. Secara histopatologi melalui pemeriksaan imunohistokimia, mutasi gen yang berperan dalam keganasan kolorektal dapat diketahui dengan cara melihat ekspresi proteinnya. [10][11][12] Meskipun ini sebagai kasus multifaktorial dapat ditinjau dari biologi molekuler keganasan kolorektal ini terjadi akibat mutasi beberapa gen seperti gen APC, K-ras, DCC, dan p53. [3][4][5] Progresivitas dan kemungkinan penyebaran keganasan kolorektal ditentukan oleh adhesi antarselnya.…”

Section: Pendahuluanunclassified

“…Penelitian lain pernah melaporkan tidak terdapat ekspresi protein tersebut berhubungan dengan respons pemberian kemoterapi. 10,12,14 Empat kasus keganasan kolorektal pada usia <40 tahun hanya didapatkan salah satu ekspresi protein gen supresor tumor (ekspresi protein DCC positif dan ekspresi protein p53 negatif atau sebaliknya). Meskipun dilakukan penelitian pada stadium yang sama (stadium III), ternyata masih mungkin didapatkan variasi hasil pemeriksaan imunohistokimia ekspresi protein tersebut pada setiap subjek yang diteliti.…”

Section: Pembahasanunclassified

See 1 more Smart Citation

Ekspresi Protein Deleted in Colorectal Cancer (DCC) dan p53 pada Keganasan Kolorektal

Adnyana¹,

Rudiman²

2017

mkb

View full text Add to dashboard Cite

AbstrakAngka kejadian keganasan kolorektal pada usia muda semakin meningkat, tetapi belum ada data yang cukup menjelaskan mengenai hal tersebut, termasuk mutasi gen yang berperan. Tujuan penelitian mengetahui apakah ekspresi protein DCC dan p53 berperan pada keganasan kolorektal usia kurang dari 40 tahun. Dilakukan pemeriksaan imunohistokimia di Departemen Patologi Anatomi Fakultas Kedokteran Universitas Padjadjaran/ Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung periode Januari sampai Mei 2011 untuk melihat ekspresi protein DCC dan p53 pada sediaan blok parafin penderita keganasan kolorektal kelompok usia <40 tahun dan usia >40 tahun. Bahan pemeriksaan dikumpulkan mulai bulan Januari sampai Mei 2011. Hasil penelitian menunjukkan bahwa pada keganasan kolorektal usia <40 tahun lebih banyak yang tidak didapatkan ekspresi kedua protein tersebut, 9 kasus pada kelompok usia >40 tahun, sedangkan pada usia lebih dari 40 tahun hanya ditemukan pada 2 kasus. Simpulan, pada sebagian besar kasus keganasan kolorektal usia <40 tahun, protein DCC dan p53 sudah tidak terekspresi. [MKB. 2017;49(3):186-91] Kata kunci: Ekspresi DCC, ekspresi p53, keganasan kolorektal, usia Deleted in Colorectal Cancer (DCC) and p53 Protein Expressions in Colorectal Cancer AbstractThe incidence of colorectal cancer in young patients is currently increasing; however, no adequate data available to explain the increase, including the gene mutation involved in these cancer. This study aimed to discover the DCC and p53 protein expressions that play a role in colorectal cancer in people under 40 years old. Immunohistochemical examination was performed at the Department of Anatomy Pathology Faculty of Medicine of Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung in the period of January to May 2011 to look into DCC and p53 expressions in paraffin blocks of colorectal malignancy patients in the age group of <40 and >40 years old. The examination specimens were collected in the period of January to May 2011. The results of the study showed that in colorectal malignancy in <40 years old patients, the expression of the two proteins was rarely found while in the age group of 40 years old, the expressions were only found in 2 cases. It is concluded in most cases of colorectal cancer in the <40 years old group, the DCC and p53 are already not expressed. [MKB. 2017;49(3):186-91]

show abstract

Deleted in Colon Cancer Protein Expression in Colorectal Cancer Metastases: A Major Predictor of Survival in Patients With Unresectable Metastatic Disease Receiving Palliative Fluorouracil-Based Chemotherapy

Abstract: Our results indicate that expression of the DCC protein in CRC metastases is similar to that observed in the corresponding primary tumors and represents a dominant predictor of survival in patients with unresectable, advanced CRC who are undergoing palliative FU-based chemotherapy.

Cited by 21 publications

References 33 publications

Prognostic and predictive factors in colorectal cancer

Prognostic and predictive factors in colorectal cancer

The gene expression profile represents the molecular nature of liver metastasis in colorectal cancer

Ekspresi Protein Deleted in Colorectal Cancer (DCC) dan p53 pada Keganasan Kolorektal

Contact Info

Product

Resources

About