Deleted in Azoospermia-Like Enhances In Vitro Derived Porcine Germ Cell Formation and Meiosis

Park, Bong-Wook; Shen, Wei; Linher‐Melville, Katja; Li, Julang

doi:10.1089/scd.2012.0323

Cited by 16 publications

(18 citation statements)

References 51 publications

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“…Consistent with previous reports (Dyce et al, 2006;Park et al, 2013;Shen et al, 2012), after culturing for 20 days some round cells appeared to 5hmC in germ cell reprogramming, is upregulated (Wu & Zhang, 2014). We also analysed the mRNA levels of Dnmt3a, Dnmt3b and Tet1, and the results revealed that while Dnmt3a and Dnmt3b were decreased, Tet1 was increased in pPGCLCs when compared with pSDSCs ( Figure 3c).…”

Section: Pgclcs Formation From Cultured Psdscssupporting

confidence: 89%

“…RA promotes the proliferation of pPGCLCs in vitro during induced differentiation During the pPGCLCs induction stage, a subpopulation of the pPGCLCs progressively detached and grew as suspended single or clustered cells(Dyce et al, 2006;Park et al, 2013;Shen et al, 2012;Figure 1). For testing the potential effects of different RA concentrations on the proliferation of pPGCLCs, a final concentration of 0, 2, 5 and 10 μM of RA was added to the differentiation medium.…”

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confidence: 99%

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RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

Yan

Liu

et al. 2019

Journal Cellular Physiology

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Previous studies have shown that primordial germ cell‐like cells (PGCLCs) can be obtained from human, porcine and mouse skin‐derived stem cells (SDSCs). In this paper, we found retinoic acid (RA), the active derivative of vitamin A, accelerated the growth of porcine primordial germ cells (pPGCs) and porcine PGCLCs (pPGCLCs) which were derived from porcine SDSCs (pSDSCs). Moreover, flow cytometry results revealed that the proliferation promoting effect of RA was attenuated by U0126, a specific inhibitor of extracellular signal‐regulated kinase (ERK). Western blot analysis showed the protein level of ERK, phosphorylated ERK, cyclin D1 (CCND1), and cyclin‐dependent kinase 2 (CDK2) increased after stimulation with RA, and this effect could also be abolished by U0126. Our data revealed that ablation of ERK expression by U0126 should significantly decrease proliferation of pPGCLCS. This reduction was because CCND1 and CDK2 proteins level decrease and subsequently the pPGCLCs were arrested in the G0/G1 phase. In addition, we also confirmed RA indeed promoted the proliferation of pPGCs isolated from porcine fetal genital ridges in vitro. Furthermore, our data indicated that DNA methylation pattern were changed in pPGCLCs and this pattern were more similar to pPGCs.

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Section: Pgclcs Formation From Cultured Psdscssupporting

confidence: 89%

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confidence: 99%

RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

Yan

Liu

et al. 2019

Journal Cellular Physiology

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“…44 Interestingly, the transfection of SDSCs with a deleted in azoospermia-like (DAZL) expression vector at the initiation of induced differentiation significantly enhanced the formation of PGCLCs (~4 times), and stimulated the expression of meiotic germ cell genes. 45 Moreover, the heparin-binding growth factor midkine was found to promote the proliferation of SDSC-derived PGCLCs and that of endogenous PGCs as well. 46 During differentiation, follicle-like structures were formed from the fetal SDSCs surrounding the OLCs.…”

Section: Open Questionsmentioning

confidence: 98%

“…45 Although these results need to be reproduced in species with a gametogenesis more similar to humans, they represent promising news for women suffering from reduced estradiol production, a common phenomenon observed after menopause transition because of the exhaustion of the ovarian reserve. 84 In this regard, transplantation of human SDSCs could be used for POF treatments.…”

Section: Open Questionsmentioning

confidence: 99%

Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells

Cheng

Dyce

et al. 2016

Cell Death Dis

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The skin is a unique organ that contains a variety of stem cells for the maintenance of skin homeostasis and the repair of skin tissues following injury and disease. Skin-derived stem cells (SDSCs) constitute a heterogeneous population of stem cells generated in vitro from dermis, which can be cultured as spherical aggregates of cells in suspension culture. Under certain in vitro or in vivo conditions, SDSCs show multipotency and can generate a variety of neural, mesodermal, and endodermal cell types such as neurons, glia, fibroblasts, adipocytes, muscle cells, chondroblasts, osteoblats, and islet β-cell-like cells. SDSCs are likely derived from multipotent stem cells located in the hair follicles that are, in turn, derived from embryonic migratory neural crest or mesoderm cells. During the past decade, a wave of reports have shown that germ cells can be generated from various types of stem cells. It has been shown that SDSCs are able to produce primordial germ cell-like cells in vitro, and even oocyte-like cells (OLCs). Whether these germ cell-like cells (GCLCs) can give rise to viable progeny remains, however, unknown. In this review, we will discuss the origin and characteristics of SDSCs from which the GCLC are derived, the possible mechanisms of this differentiation process, and finally the prospective biomedical applications of the SDSC-derived GCLCs.

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“…In previous studies, skin has been shown as an excellent adult stem cell source, which differentiated into osteogenic and neural cells in vitro and enhanced bone formation in the maxillary sinus floor and nerve regeneration in vivo 5,6. Further, stem cells derived from fetus differentiated into primordial germ cell and oocyte, and exhibited similar characteristics compared to embryonic stem cells7,8. However, harvesting skin tissue also involves an invasive procedure, and stem cells from the skin of older donors possess less stemness and regenerative potential than that of younger donors4,5.…”

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confidence: 94%

Dental tissues as adult stem cell source

Park

2013

J Korean Assoc Oral Maxillofac Surg

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Deleted in Azoospermia-Like Enhances In Vitro Derived Porcine Germ Cell Formation and Meiosis

Cited by 16 publications

References 51 publications

RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

RA promotes proliferation of primordial germ cell‐like cells differentiated from porcine skin‐derived stem cells

Skin-derived stem cells as a source of primordial germ cell- and oocyte-like cells

Dental tissues as adult stem cell source

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