Delayed tooth movement in Runx2+/− mice associated with mTORC2 in stretch-induced bone formation

Aonuma, Tomo; Tamamura, Nagato; Fukunaga, Tomohiro; Sakai, Yuichi; Takeshita, Nobuo; Shigemi, Shohei; Yamashiro, Takashi; Takano‐Yamamoto, Teruko

doi:10.1016/j.bonr.2020.100285

Cited by 8 publications

(4 citation statements)

References 61 publications

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“…Therefore, this experimental tooth movement model simulated orthodontic tooth movement in clinical practice. Our results were consistent with previous reports of orthodontic tooth movement in mouse or rat 6 , 31 , 32 , 47 , 48 . Furthermore, no change in body weight was observed between the experimental tooth movement and control groups during the experimental period, suggesting that the experimentally induced tooth movement caused minimal stress to the rats.…”

Section: Discussionsupporting

confidence: 94%

The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats

Shigemi,

Sato,

Sakamoto

et al. 2023

Sci Rep

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When orthodontic forces are applied to teeth, bone remodeling, which consists of bone resorption and bone formation, occurs around the teeth. Transient receptor potential vanilloid 2 (TRPV2) is a cation channel expressed in various cell types that responds to various stimuli, including mechanical stress, and involved in calcium oscillations during the early stages of osteoclast differentiation. However, in vivo expression of TRPV2 in osteoclasts has not yet been reported, and temporo-spatial expression of TRPV2 during osteoclast differentiation is unclear. In this study, we examined the TRPV2 expression during experimental tooth movement and assessed the effect of TRPV2 on osteoclast differentiation. TRPV2 was detected on day 1 after experimental tooth movement on the compression side, and the number of TRPV2-expressing cells significantly increased on day 7. These TRPV2-expressing cells had a single, or multiple nuclei and were positive for TRAP activity. Consistent with these in vivo findings, in vitro experiments using RAW264.7 osteoclast progenitor cells showed that TRPV2 mRNA was increased at the early stage of osteoclast differentiation and maintained until the late stage. Furthermore, a TRPV2 channel selective antagonist significantly inhibited osteoclast differentiation. These findings suggest that TRPV2 may have a regulatory role in osteoclast differentiation during orthodontic tooth movement.

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Section: Discussionsupporting

confidence: 94%

The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats

Shigemi,

Sato,

Sakamoto

et al. 2023

Sci Rep

Self Cite

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“…For example, mTOR is regulated by Runx2 to phosphorylate Akt for modulating cell proliferation and differentiation in OBs and BMSCs on the tension side during OTM in vivo and in vitro. Aonuma reported that Runx2(+/-) mice exhibited suppressed mTORC2/Akt activity 127 . P70S6 K as a downstream molecule of mTOR is activated by phosphorylation and subsequently promotes the synthesis of ribosomal and translational proteins.…”

Section: Fig 3 Hypothetical Mechanisms Of Mechanical Signal Transduct...mentioning

confidence: 99%

Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade

et al. 2021

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Nowadays, orthodontic treatment has become increasingly popular. However, the biological mechanisms of orthodontic tooth movement (OTM) have not been fully elucidated. We were aiming to summarize the evidences regarding the mechanisms of OTM. Firstly, we introduced the research models as a basis for further discussion of mechanisms. Secondly, we proposed a new hypothesis regarding the primary roles of periodontal ligament cells (PDLCs) and osteocytes involved in OTM mechanisms and summarized the biomechanical and biological responses of the periodontium in OTM through four steps, basically in OTM temporal sequences, as follows: (1) Extracellular mechanobiology of periodontium: biological, mechanical, and material changes of acellular components in periodontium under orthodontic forces were introduced. (2) Cell strain: the sensing, transduction, and regulation of mechanical stimuli in PDLCs and osteocytes. (3) Cell activation and differentiation: the activation and differentiation mechanisms of osteoblast and osteoclast, the force-induced sterile inflammation, and the communication networks consisting of sensors and effectors. (4) Tissue remodeling: the remodeling of bone and periodontal ligament (PDL) in the compression side and tension side responding to mechanical stimuli and root resorption. Lastly, we talked about the clinical implications of the updated OTM mechanisms, regarding optimal orthodontic force (OOF), acceleration of OTM, and prevention of root resorption.

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“…The activity of alkaline phosphatase (ALP) and calcium content were evaluated according to the methods of previous studies [ 38 , 39 ], with some modifications. ALP activity and calcium content were examined according to the protocols of the alkaline phosphatase assay kit (Cat.…”

Section: Methodsmentioning

confidence: 99%

Three-Dimensional Printing of Polycaprolactone/Nano-Hydroxyapatite Composite Scaffolds with a Pore Size of 300/500 µm is Histocompatible and Promotes Osteogenesis Using Rabbit Cortical Bone Marrow Stem Cells

Yang,

Qiu,

Zhou

et al. 2023

Ann Transplant

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Background Many patients have bone defects that exceed the healing size. This study aimed to construct polycaprolactone/nano-hydroxyapatite (PCL/nHA) composite scaffolds with different pore sizes and investigate the osteogenesis and histocompatibility of cortical bone mesenchymal stem cells (BMSCs-C) seeded on it after inoculation. Material/Methods After mixing PCL and nHA proportionally, three-dimensional (3D) printing was used to print scaffolds. Porosity, compressive strength, and elastic modulus of PCL/nHA scaffolds were tested. The proliferation of BMSCs-C cells was examined and osteogenesis, chondrogenesis, and adipogenesis were evaluated. BMSCs-C cells were inoculated into 3D printing scaffolds, and histocompatibility between BMSCs-C cells and scaffolds was observed by the cell count kit (CCK-8) assay and LIVE/DEAD staining. After inoculating BMSCs-C cells into scaffolds, alkaline phosphatase (ALP) activity and calcium content were measured. Results There was no obvious difference in characteristics between the 3 PCL/nHA composite scaffolds. The porosity, compressive strength, and elastic modulus of the 300/500-μm scaffold were between those of the 300-μm and 500-μm scaffolds. With increasing pore size, the mechanical properties of the scaffold decrease. BMSCs-C cells demonstrated faster growth and better osteogenic, adipogenic, and chondrogenic differentiation; therefore, BMSCs-C cells were selected as seed cells. PCL/nHA composite scaffolds with different pore sizes had no obvious toxicity and demonstrated good biocompatibility. All scaffolds showed higher ALP activity and calcium content. Conclusions The 300/500 μm mixed pore size scaffold took into account the mechanical properties of the 300 μm scaffold and the cell culture area of the 500 μm scaffold, therefore, 300/500 μm scaffold is a better model for the construction of tissue engineering scaffolds.

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Delayed tooth movement in Runx2+/− mice associated with mTORC2 in stretch-induced bone formation

Cited by 8 publications

References 61 publications

The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats

The role of TRPV2 as a regulator on the osteoclast differentiation during orthodontic tooth movement in rats

Biomechanical and biological responses of periodontium in orthodontic tooth movement: up-date in a new decade

Three-Dimensional Printing of Polycaprolactone/Nano-Hydroxyapatite Composite Scaffolds with a Pore Size of 300/500 µm is Histocompatible and Promotes Osteogenesis Using Rabbit Cortical Bone Marrow Stem Cells

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