Delayed Tattoo Reaction From Red Dye With Overlapping Clinicopathological Features: Examination With High-Frequency Ultrasound and Line-Field Optical Coherence Tomography

Tognetti, Linda; Ekinde, Sean; Habougit, Cyril; Cinotti, Élisa; Rubegni, Pietro; Perrot, Jean

doi:10.5826/dpc.1003a53

Cited by 10 publications

(8 citation statements)

References 2 publications

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“…The features of basal cell carcinoma (BCC), actinic keratosis, squamous cell carcinoma, sebaceous hyperplasia, xanthogranuloma, herpes, scabies, Kaposi sarcoma, aquagenic keratoderma, bullous diseases and normal skin have been described with this technology. [2][3][4][5][6][7][8][9][10][11][12][13][14] RCM and OCT are two other noninvasive technologies, which have different resolutions and depths of penetration into the skin. RCM enables an en face view with cellular resolution (spatial lateral resolution of 1 lm, similar to histology and axial resolution of < 5 lm).…”

Section: Introductionmentioning

confidence: 99%

Morphological evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

Pérez-Ánker

Puig

Alós

et al. 2022

Clinical and Experimental Dermatology

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Background Line‐field confocal optical coherence tomography (LC‐OCT) is a new in vivo emerging technique that provides cellular resolution, allows deep imaging (400 μm) and produces real‐time images in both the horizontal and vertical plane and in three dimensions. No previous description of different subtypes of melanocytic lesions and their correlation with histopathology and reflectance confocal microscopy has been reported. Aim To describe the features of melanocytic lesions by LC‐OCT and their correlation with histopathology and reflectance confocal microscopy (RCM) findings. Methods Selected melanocytic benign lesions and melanomas were imaged in vivo with RCM and LC‐OCT at the Fundación Hospital Clinic (Barcelona, Spain). A minimum area of 4 × 4 mm (block image) at four depths (stratum granulosum, suprabasal, layer dermoepidermal junction and upper dermis) were acquired with RCM and a minimum of three cubes with LC‐OCT. Horizontal, vertical sections and three‐dimensional (3D) cubes of LC‐OCT were matched with RCM (Vivablock two‐dimensional composite mosaic) and histopathology, with ~5 μm lateral resolution accuracy (the same cell nuclei were measured in X, Y and Z) and evaluated by three observers experienced in using RCM and histopathology. Results In total, 12 melanocytic tumours (2 in situ melanomas, 2 invasive melanomas, 4 atypical naevi, 2 intradermal naevi, 1 compound naevus and 1 junctional naevus) were included. High correlation with 5 μm accuracy between RCM and LC‐OCT was observed for each tumour. The 3D images of melanocytic lesions were obtained with cellular resolution and correlated with both RCM and histopathology, allowing an understanding of the architecture and precise correlation at the cellular level with RCM. Similarities between LC‐OCT and RCM for the described diagnostic features and architecture (nests of melanocytic cells, ringed and meshwork pattern, and cellular details of tumour cells as dendritic and pagetoid cells) were confirmed. The main advantage of diagnosis by RCM fixed probe was the ability to produce larger scans of the lesion using mosaicing compared with an LC‐OCT handheld probe. Conclusion LC‐OCT allows the architectural and cellular description of different types of melanocytic lesions. LC‐OCT showed high correlation with histopathology (vertical sections) and RCM (horizontal sections) in melanocytic lesions. Diagnostic criteria for RCM were similar to those for LC‐OCT.

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Section: Introductionmentioning

confidence: 99%

Morphological evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

Pérez-Ánker

Puig

Alós

et al. 2022

Clinical and Experimental Dermatology

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“…[8][9][10][11][12][13][14][15][16][17][18][19] The device is based on a supercontinuum laser, that, briefly, combines the technological advances of reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) in terms of cellular and spatial resolution and vertical/ horizontal navigation. Our group has successfully used LC-OCT to investigate the morphology of epidermis and adnexal structures in both healthy skin, 8 skin cancers and their simulators, [9][10][11] vesicobullous 12 and inflammatory conditions, 13 rare epidermal diseases [14][15][16][17] and uncommon paediatric conditions. 18,19 The accuracy of RCM in characterizing pustular rashes was previously demonstrated by Debarbieux et al, 7 while standard OCT was employed to characterize Malassezia folliculitis pustules, 20 and dermoscopy was employed to imagine acute severe erythemato-pustular rashes.…”

Section: Introductionmentioning

confidence: 99%

“…The line‐field confocal optical coherence tomography (LC‐OCT) is a recently developed imaging technique able to perform a non‐invasive, in vivo , examination of the epidermis and upper dermis, reaching very high image resolution and virtual histology 8–19 . The device is based on a supercontinuum laser, that, briefly, combines the technological advances of reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) in terms of cellular and spatial resolution and vertical/horizontal navigation.…”

Section: Introductionmentioning

confidence: 99%

“…The line-field confocal optical coherence tomography (LC-OCT) is a recently developed imaging technique able to perform a non-invasive, in vivo, examination of the epidermis and upper dermis, reaching very high image resolution and virtual histology. [8][9][10][11][12][13][14][15][16][17][18][19] The device is based on a supercontinuum laser, that, briefly, combines the technological advances of reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) in terms of cellular and spatial resolution and vertical/ horizontal navigation. Our group has successfully used LC-OCT to investigate the morphology of epidermis and adnexal structures in both healthy skin, 8 skin cancers and their simulators, [9][10][11] vesicobullous 12 and inflammatory conditions, 13 rare epidermal diseases [14][15][16][17] and uncommon paediatric conditions.…”

Section: Introductionmentioning

confidence: 99%

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Line‐field confocal optical coherence tomography: a new tool for non‐invasive differential diagnosis of pustular skin disorders

Tognetti

Cinotti

Falcinelli

et al. 2022

Acad Dermatol Venereol

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Background The spectrum of pustular skin disorders (PSD) is large and particularly challenging, including inflammatory, infectious and amicrobial diseases. Moreover, although pustules represent the unifying clinical feature, they can be absent or not fully developed in the early stage of the disease. The line-field confocal optical coherence tomography (LC-OCT) is a recently developed imaging technique able to perform a non-invasive, in vivo, examination of the epidermis and upper dermis, reaching very high image resolution and virtual histology.Objectives We aimed to investigate the potentialities of LC-OCT in the non-invasive differential diagnosis of a series of 11 PSD with different aetiology, microscopic features, body location and incidence rates. Materials and MethodsComplete LC-OCT imaging (i.e. 2D/3D frames, videos) was performed on a total of 19 patients (10 females and 9 males) aged between 35 and 79 years. Images were blindly evaluated and compared with corresponding histopathologic findings. ResultsThe LC-OCT imaging was able to detect with high accuracy the pustule structure including shape, margins, morphology and cellular content, along with peculiar epidermal and adnexal alterations in each condition, including: Acute Generalized Exanthematous Pustulosis, Generalized pustular psoriasis, Generalized pustular figurate erythema, Subcorneal Pustular Dermatosis, Intraepidermal IgA pustulosis, Palmoplantar pustulosis, Palmoplantar pustular psoriasis. Herpetic whitlow, Acrodermatitis continua of Hallopeau, Vesicopustular Sweet syndrome and Vesicopustular Eosinophilic cellulitis, with pustular appearance, were also compared. ConclusionsThe new LC-OCT can represent a rapid, non-invasive and painless tool which can help differentiating among PSD of different aetiology and microscopic morphology in clinical mimickers in daily practice.

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“…We previously demonstrated the ability of LC-OCT to show the in vivo skin structure of healthy skin, with a clear definition of the epidermal layers and a depth of imaging reaching the deeper/papillary dermis [17][18][19][20][21][22][23]. Then, LC-OCT was successfully used to investigate the morphology of skin cancers [16,18,24,25] and rare skin diseases including inflammatory, [19,20] vascular structures [16][17][18] and morpho-functional alteration of stratum corneum and adnexa [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Line field confocal optical coherence tomography: An adjunctive tool in the diagnosis of autoimmune bullous diseases

Tognetti

Cinotti

Suppa

et al. 2021

Journal of Biophotonics

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Autoimmune bullous diseases (AIBDs)still represent a considerable a source of morbidity and mortality: early identification of a specific AIBD is often difficult due to overlapping clinical and/or laboratory features and time-consuming invasive laboratory tests. We aimed to investigate the potential role of a new imaging technology, line-field confocal optical coherence tomography (LC-OCT), in the non-invasive diagnosis of AIBDs. LC-OCT was performed at lesional, perilesional and contralateral healthy sites in 30 patients, before histology and direct immunofluorescence. LC-OCT examination was able to identify the level of split (subcorneal/ suprabasal/subepidermal/sublamina densa), to provide detailed images of the bulla roof morphology and content (eg, erythrocytes/acantholytic cells/polymorphonucleates). Areas of intra/subepidermal detachment were also detected also at clinically normal perilesional skin sites. LC-OCT can support physicians, real time and at bed-site, in the differential diagnosis of various AIBDs and their mimickers. Moreover, it can be used for the identification of subclinical lesions and therapy tapering.

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Delayed Tattoo Reaction From Red Dye With Overlapping Clinicopathological Features: Examination With High-Frequency Ultrasound and Line-Field Optical Coherence Tomography

Cited by 10 publications

References 2 publications

Morphological evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

Morphological evaluation of melanocytic lesions with three-dimensional line-field confocal optical coherence tomography: correlation with histopathology and reflectance confocal microscopy. A pilot study

Line‐field confocal optical coherence tomography: a new tool for non‐invasive differential diagnosis of pustular skin disorders

Line field confocal optical coherence tomography: An adjunctive tool in the diagnosis of autoimmune bullous diseases

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