Delayed skin reactions to inhalant antigens

Green, George R.; Zweiman, Burton; Beerman, Herman; Hildreth, Eugene A.

doi:10.1016/0021-8707(67)90085-8

Cited by 32 publications

(13 citation statements)

References 8 publications

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“…This finding contrasts with the reports of others (4)(5)(6)22). The explanation for this is not known, but other workers have also experienced similar difficulties in attempting to demonstrate delayed hypersensitivity skin tests (23).…”

Section: Discussioncontrasting

confidence: 68%

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Rocklin¹,

Pence²,

Kaplan³

et al. 1974

J. Clin. Invest.

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A B S T R A C T The in vivo and in vitro responses to ragweed antigen E were evaluated in 28 untreated atopic patients with ragweed hayfever. The methods employed included direct skin testing, measurement of total serum IgE, measurement of specific IgE anti-ragweed antibodies, leukocyte histamine release, lymphocyte transformation, and release of lymphocyte mediators (migration inhibitory factor and mitogenic factor). The patients could be divided into sensitive and insensitive groups on the basis of their in vitro reactivity to antigen E. 20 patients in the sensitive group had statistically higher levels of total serum IgE, higher levels of specific IgE anti-ragweed antibodies, and greater leukocyte sensitivity as measured by antigen-induced histamine release than did eight patients in the insensitive group. Lymphocytes from sensitive patients produced greater amounts of migration inhibitory factor and mitogenic factor when challenged by antigen E than did lymphocytes from insensitive patients. A possible role for the lymphocyte in this allergic disease is discussed. serum IgE levels of paIgEAR levels but normal total serum IgE levels. Of interest was the finding that a small number of patients had diminished lymphocyte mediator production, histamine release, IgE, and IgEAR levels. One could not distinguish clinically between these two groups. METHODSPatients. 28 atopic patients with symptoms compatible with seasonal allergic rhinitis and no previous history of receiving ragweed immunotherapy were included in this study. The ages ranged from 15 to 64 yr (mean 28.9 yr) and included 23 males and 5 females. 18 nonatopic subjects served as controls.Skin tests. At one forearm site 0.05 ml of aqueous ragweed extract (100 protein nitrogen units/ml) alone was injected intradermally. In the opposite forearm 0.05 ml chlorpheniramine maleate (10 mg/ml) was injected before the subsequent intradermal injection of 0.05 ml ragweed extract at the same site. At a third site chlorpheniramine alone was injected (0.05 ml). The amount of erythema and induration were measured at each site at 15 min and 48 h. In one subject a punch biopsy specimen was obtained at 48 h from sites injected with ragweed extract plus chlorpheniramine and chlorpheniramine alone.Serum IgE levels. Serum samples were obtained from patients at the time of initial evaluation and IgE levels determined by use of a Phadebas kit purchased from Pharmacia (Uppsala, Sweden). Serum samples for IgE levels were also obtained from 50 subjects in the same age range as the patient population.Specific IgEAR levels. The radioallergosorbent test (RAST) was used to measure specific IgEAR levels (9). 10 ml of a 20% whole ragweed extract was conjugated to 500 mg of cyanogen bromide-activated cellulose particles (10). 25-, 50-, and 75-il samples of sera were added to 0.5-mg aliquots of the antigen-cellulose conjugate and incubated overnight in a horizontal rotator. After washing, 0.05 ml of "I-labeled immunosorbent purified anti-IgE (Fc) was added to each tube. The anti-IgE (...

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Section: Discussioncontrasting

confidence: 68%

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Rocklin¹,

Pence²,

Kaplan³

et al. 1974

J. Clin. Invest.

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“…Marked infiltration with many mononuclear cells was the predominant feature. In another study (23) skin biopsies performed 48 hours after skin tests with common inhalant antigens showed histologic findings indistinguishable from those of tuberculin reactions, with dense perivascular and interstitial round cell infiltrations in delayed skin reactors.…”

Section: Discussionmentioning

confidence: 94%

Relationship of Dermal and Pulmonary Sensitivity to Extracts ofCandida albicans¹

Kabe¹,

Aoki²,

Ishizaki³

et al. 1971

Am Rev Respir Dis

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“…Late skin reactions to atopic allergens are well documented [3,7]. Late responses to provocative allergen chal lenge in both nose and bronchi have been reported [8,19].…”

Section: Discussionmentioning

confidence: 99%

Studies of the Radioallergosorbent Test and the Lymphocyte Stimulation Index in Symptomatic Ragweed Pollenosis

Yoo¹,

Kuo²,

Rajtora³

et al. 1978

Int Arch Allergy Immunol

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We followed 47 ragweed hay fever patients (not on immunotherapy) through a ragweed season by means of clinical evaluation and symptom scores. The radioallergosorbent test (RAST) and lymphocyte stimulation index (LSI) were done to determine their reliability as predictors of clinical symptomatology. Symptom scores of 10 or above, denoting at least moderate daily symptoms, occurred in 36 patients (80%). Preseasonally, RAST class 2 or above was found in 34 patients (75%), of whom 29 patients (64%) had symptom scores of 10 or above. At that time, LSI was elevated in about 50% of the patients. Patients in RAST class 3 had the highest LSI as a group. Seasonal exposure boosted the LSI in 50% of the patients, whereas 25% were unchanged and 25% showed a decrease. The results suggest that neither RAST nor LSI are reliable predictors of clinical severity in ragweed pollenosis.

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Delayed skin reactions to inhalant antigens

Cited by 32 publications

References 8 publications

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Relationship of Dermal and Pulmonary Sensitivity to Extracts ofCandida albicans¹

Studies of the Radioallergosorbent Test and the Lymphocyte Stimulation Index in Symptomatic Ragweed Pollenosis

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Delayed skin reactions to inhalant antigens

Cited by 32 publications

References 8 publications

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Cell-Mediated Immune Response of Ragweed-Sensitive Patients to Ragweed Antigen E

Relationship of Dermal and Pulmonary Sensitivity to Extracts ofCandida albicans1

Studies of the Radioallergosorbent Test and the Lymphocyte Stimulation Index in Symptomatic Ragweed Pollenosis

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Relationship of Dermal and Pulmonary Sensitivity to Extracts ofCandida albicans¹