Four rats were each fixed with two chronically indwelling bipolar electrodes for intracranial stimulation (ICS). ICS with one bipolar electrode was rewarding, and ICS with the other was aversive. After demonstration of the efficacy of ICS, rats were placed in a chamber with a retractable lever. A depression of the lever yielded four trains of ICS. All possible combinations of positive and negative ICS within the constraint of four events were then tested with high intensities of both rewarding and aversive ICS at intertrain intervals of .125, .25, and 1.0 sec, and with different intensities of ICS. It was found that the rats pressed more for the contingencies containing the greater number of positive ICS. The sequence of rewarding and aversive ICS was also shown to be an important variable in determining pressing rates. It was concluded that knowing the net positiveness of complex contingencies and knowing whether the first event was rewarding or aversive was sufficient to predict closely the acceptability of a contingency.Behavior is frequently followed by more than one or two affective even ts. Available laboratory data, however, are limited to parametric studies using a single positive reinforcer, a single aversive event, or a combination of the two. The demonstrations that intracranial stimulations (ICS) can control behavior as conventional affective stimulations (Buckwalter, Gibson, Reid, & Porter, 1967;Gibson, Reid, Sakai, & Porter, 1965 ;Hunsicker & Reid, 1974;Olds, 1969; Reid, Hunsicker, Kent. Johnson, & Gallistel, 1973) opens the way to using ICS as a tool for studying more complex contingencies. This study, consequen tJy, employed ICS to determine how leverpressing was controlled by sequences of affective events. Rats fixed with bipolar electrode for rewarding, positive ICS and an electrode pair for aversive, r:egative ICS were allowed to press a lever for four ICSs in all possible combinations of positive and negative, e.g., positive-positive-negativenegative and negative-negative-positive-positive were among the 16 sequences tested.
METHOD
SubjectsFour experimentally naive adult male Sprague-Dawley rats were each lixed, in standard ways, with two chronically indwelling bipolar electrodes. The electrodes were stainless steel and insulated except at the cross section of their tips. The tips of each electrode of an electrode pair were separated only by the width of each electrode's insulation. Histological analyses of frozen sections of the brains of the rats, following behavioral testing, conlirmed that ICS with one clectrode pair stimulated the medial forebrain bundle of the lateral hypothalamus. ICS with the other electrode pair stimulated an area near the geniculates and the geniculates themselves. Other studies have shown previously that these sites of stimulation are, respectively, rewarding and aversivc (Olds, 1962 ; Buckwalter et ai, 1967). Prior to testing in the apparatus, it was conlirmcd that the 'Supported by Bradley University by way of its Board for Research which administers NSF Grant ...