Delayed osteoblast differentiation and altered inflammatory response around implants placed in incisor sockets of type 2 diabetic rats

Colombo, John S.; Balani, Deepak H.; Sloan, Alastair James; Crean, St John; Okazaki, Joji; Waddington, Rachel J.

doi:10.1111/j.1600-0501.2010.01992.x

Cited by 51 publications

(47 citation statements)

References 44 publications

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Section: Abbreviations Used In This Papermentioning

confidence: 99%

“…Animal studies using models of type 2 diabetes have histologically demonstrated a delay in the early stages of bone formation [Casap et al, 2008;Hasegawa et al, 2008;Colombo et al, 2010]. Using immunohistochemistry to monitor the events of bone healing around implants placed into the mandibles of diabetic GotoKakizaki (GK) rats, our studies have demonstrated that, while the infiltration of stro-1-positive mesenchymal cells into the granulation tissue appeared to be unaffected, the onset of cell proliferation is delayed and subsequently prolonged in diabetic healing bone compared to normal bone [Colombo et al, 2010]. Similarly, the production of IL-1 ␤ , TNF-␣ and TGF-␤ 1 (signalling molecules associated with the wound healing process), as well as the presence of macrophages and osteoblast differentiation (monitored by the temporal appearance of the bone matrix proteins, osteopontin and osteocalcin), were initially seen to be delayed and then prolonged [Colombo et al, 2010].…”

Section: Abbreviations Used In This Papermentioning

confidence: 99%

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Characterization of Oxidative Stress Status during Diabetic Bone Healing

Waddington

Alraies

Colombo

et al. 2011

Cells Tissues Organs

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Early events associated with bone healing in patients with type 2 diabetes mellitus appear to be delayed. Hyperglycaemia and an associated increase in oxidative stress are cited as potential factors leading to a change in cellular behaviour. Using an in vivo model monitoring bone formation around implants placed into rat mandibles, we have previously identified that the onset of cell proliferation and osteoblast differentiation are delayed and subsequently prolonged compared with normal bone. This study used the same implant model to characterize oxidative stress biomarkers and primary antioxidant enzyme profiles during diabetic bone healing in vivo. Implants were placed into the sockets of incisors extracted from the mandibles of normal Wistar and diabetic Goto-Kakizaki rats for 3 and 9 weeks after implant insertion. Histochemical analysis confirmed a delay in bone healing around implants in diabetic animals. Immunohistochemical localization of peri-cellular staining for protein carbonyl groups, as a biomarker of oxidized protein content, was slightly higher in diabetic granulation tissue compared with normal tissue. However, no differences were observed in the staining patterns of advanced glycation end products. Minimal differences were observed in the number of cells positive for cytoplasmic superoxide dismutase (SOD)1 or mitochondrial SOD2. Significantly, catalase was absent in diabetic tissues. The results suggest that the oxidative environment in healing bone is differentially affected by hyperglycaemia, particularly in relation to catalase. The significance of these observations for diabetic bone healing is discussed.

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Section: Abbreviations Used In This Papermentioning

confidence: 99%

Section: Abbreviations Used In This Papermentioning

confidence: 99%

Characterization of Oxidative Stress Status during Diabetic Bone Healing

Waddington

Alraies

Colombo

et al. 2011

Cells Tissues Organs

Self Cite

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“…Previous animal studies also support this conclusion. in vivo studies have reported unfavorable socket healing and alveolar bone destruction in diabetic rodent models . Decreased osteoblast differentiation and mineral deposition rates contributing to delayed healing have been observed in diabetic rodent models .…”

Section: Discussionmentioning

confidence: 99%

“…T2DM is characterized by hyperglycemia due to either the relative lack of insulin or resistance to insulin, which is associated with a series of skeletal complications, including poor osseous healing and impaired bone regeneration . Reports based on diabetic animal models have suggested that the delayed healing of postextraction sockets is associated with unfavorable socket dimensions postextraction . However, reports based on observations in humans (ie, diabetic patients and/or diabetic patients under glycemic management) indicate controversial effects of diabetes on the postextraction socket and ridge .…”

Section: Introductionmentioning

confidence: 99%

Type 2 diabetes affects postextraction socket healing and influences first‐stage implant surgery: A study based on clinical and animal evidence

Zhang

Song

Wang

et al. 2019

Clin Implant Dent Rel Res

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AIM To verify the influence of type 2 diabetes mellitus (T2DM) on postextraction socket healing and subsequent first‐stage implant surgery. Materials and Methods We analyzed pre‐extraction and postextraction cone beam computed tomography images of T2DM patients (n = 75) and paired nondiabetic controls to investigate changes in postextraction socket and ridge dimensions. The types of guided bone regeneration (GBR) surgeries were also compared. Three T2DM pig models were established to compare their postextraction socket healing with that of nondiabetic controls. Healing was quantitatively verified by microcomputed tomography. The osteogenic differentiation of mesenchymal stem cells (MSCs) was also compared. Results Compared to nondiabetic controls, T2DM patients had higher socket width/depth values postextraction across all groups with different healing times. Among the T2DM patients, 62.7% could not receive first‐stage implant surgery within 6 months postextraction, and 54.7% received GBR surgery during first‐stage surgery. Ossification was not achieved in the socket center of the T2DM pig models after 3 months of healing. A decrease in osteogenic differentiation was observed in T2DM‐MSCs. Conclusions T2DM interferes with the healing of the extraction socket and thus delays first‐stage implant surgery. This phenomenon may be due to the reduced osteogenic differentiation of MSCs in the sockets.

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“…Il topo non è incluso nella lista; tuttavia, sono numerosi gli studi che lo impiegano come modello [20][21][22][23][24][25][26][27][28] .…”

Section: Microstruttura Ossea Comparata In Alcuni Modelli Animaliunclassified

Microstruttura e funzione del tessuto osseo. Parte II: carico immediato e biomateriali

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Gherlone

Capparè

2012

Italian Oral Surgery

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Delayed osteoblast differentiation and altered inflammatory response around implants placed in incisor sockets of type 2 diabetic rats

Cited by 51 publications

References 44 publications

Characterization of Oxidative Stress Status during Diabetic Bone Healing

Characterization of Oxidative Stress Status during Diabetic Bone Healing

Type 2 diabetes affects postextraction socket healing and influences first‐stage implant surgery: A study based on clinical and animal evidence

Microstruttura e funzione del tessuto osseo. Parte II: carico immediato e biomateriali

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