Delayed-Hypersensitivity Reactions to Soluble Membrane Antigens of Human Malignant Lung Cells2

Hollinshead, Ariel C.; Stewart, T. H. M.; Herberman, Ronald B.

doi:10.1093/jnci/52.2.327

Cited by 83 publications

(23 citation statements)

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“…Hollinshead et al (1974Hollinshead et al ( , 1975) using a partially purified lung TAMA isolated by sequential low-frequency sonication, demonstrated its reactivity by skin testing. Other investigators, using more crude lung TAMA generated by 3M KCI solubilization, have demonstrated cell-mediated immune (CMI) reactivity of lung-cancer patients to such preparations.…”

Section: Discussionmentioning

confidence: 99%

“…An important observation regarding lung TAMA-1 was its apparent denaturation by treatment with 3M KCI but not by sequential low-frequency sonication, a protocol reported to isolate lung TAMA (Hollinshead et al, 1974(Hollinshead et al, , 1975. Such data emphasizes that different modalities of membrane solubilization may yield different TAA.…”

Section: Discussionmentioning

confidence: 99%

“…Membrane fractions were also treated by 3M KCI extraction according to the method of Meltzer et al (1971) and by sequential sonic disruption as per Hollinshead et al (1974) and Davies (1966). The exact procedures used were reported in detail earlier by Veltri et al (1977).…”

Section: Methodsmentioning

confidence: 99%

“…Alternatively, lung TAA may be membrane associated and present in lower concentrations making them more difficult to isolate than the soluble lung TAA. Hollinshead et al (1974Hollinshead et al ( , 1975) extracted a membrane-bound antigen by lowfrequency sonication, and partially purified the substance by column chromatography and polyacrylamide-gel electrophoresis. The demonstration of lung TAA in the circulation of lung-cancer patients seems to be confined to the soluble type of lung TAA (Viza et al, 1975;DeCarvalho, 1973;Maxim et al, 1976).…”

mentioning

confidence: 99%

“…The demonstration of lung TAA in the circulation of lung-cancer patients seems to be confined to the soluble type of lung TAA (Viza et al, 1975;DeCarvalho, 1973;Maxim et al, 1976). Finally, the demonstration of a cell-mediated immune response to human lung TAA has involved the use of membrane-extractable antigens and has used in vivo skin testing (Hollinshead et al, 1974) and in vitro methods McCoy et al, 1977;Dean et al, 1978;Pierce & DeVald, 1975;Roth et al, 1975).…”

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confidence: 99%

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A human tumour-associated membrane antigen from squamous-cell carcinoma of the lung

Veltri¹,

Maxim²,

Boehlecke³

1980

Br J Cancer

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Summary.-Primary human squamous-cell lung tumours were disrupted using mechanical high-speed homogenization. Crude membranes were isolated by differential centrifugation at low speeds followed by 100,000 g centrifugation. Such membrane preparations were extracted with Triton-X-100 and passed over a DEAE cellulose column; the DEAE unbound fraction and the bound fraction eluted with 0-4M NaCl were used to immunize rabbits. We present here only data on a single lung-tumour-associated membrane antigen (TAMA) found in the unbound DEAE cellulose column eluates. This new Triton-X-100 extractable antigen is termed lung TAMA-1.A further purification of the antigen was achieved using two methods. The first used G-200 molecular-seive chromatography and this revealed lung TAMA-1 to have a mol. wt of 200,000. A second method used sucrose density-gradient isoelectric focusing, and the antigen had an isoelectric point of -3 0.Several important properties of the lung TAMA-1 were determined. The antigen has a cathodal gamma-type electrophoretic mobility at pH 7-6. The antigen was not detected in any normal human adult or foetal tissue extracts tested. It did not crossreact immunochemically with CEA, AFP or p2 microglobulin. Lung TAMA-1 was detected in 80% of lung tumour Triton-X-100 extracts tested by counterimmunoelectrophoresis (CIEP) but was undetectable in breast or colon carcinoma extracts.Low frequency sonication did not deleteriously affect lung TAMA-1, but, 3M KCI eliminated its immunologic reactivity in CIEP. Finally, preliminary data were obtained using immunohistochemistry to localize in vivo lung TAMA-1 production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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A human tumour-associated membrane antigen from squamous-cell carcinoma of the lung

Veltri¹,

Maxim²,

Boehlecke³

1980

Br J Cancer

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Review: Perioperative therapy for locoregional nonsmall-cell lung cancer

Takita

1996

J. Surg. Oncol.

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Surgical therapy remains the treatment of choice for resectable nonsmall‐cell lung cancer (NSCLC). However, the 5‐year survival results of surgical therapy is 40–70%, which is far from acceptable. In this report, past results of perioperative therapies were reviewed to identify the future direction of effort in improving the therapy of NSCLC. Two perioperative modes of treatment that may possibly improve postsurgical survival were identified, i.e., neoadjuvant chemotherapy for resectable NSCLC and postoperative specific active immunotherapy. © 1996 Wiley‐Liss, Inc.

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