Delayed Fluorescence

Berden-Zrimec, Maja; Drinovec, Luka; Zrimec, Alexis

doi:10.1007/978-90-481-9268-7_14

Cited by 17 publications

(16 citation statements)

References 45 publications

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“…Pi-limitation has also been detected using delayed chl a fluorescence in Scenedesmus obtusiusculus (Mellvig & Tillberg, 1986). In delayed chl a fluorescence (emitted light after a period of illumination) the effect of a nutrient limitation does not primarily affect the extent of the chl a fluorescence response, but rather the timing of the delayed fluorescence peak (Berden-Zrimec et al, 2011), as shown in Dunaliella tertiolecta in response to N-limitation (Berden -Zrimec et al, 2008). After two days of growth, Pi-replete cells were growing at high rates (0.94 ± 0.02 d -1 and 0.64 ± 0.03 d -1 for high and low CO 2 cultures, respectively) that lie well within the range reported previously (Spijkerman, 2005(Spijkerman, , 2008bTittel et al, 2005).…”

Section: Effect Of Pmentioning

confidence: 98%

Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga

Spijkerman

Stojkovic

Holland

et al. 2015

European Journal of Phycology

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Nutrient Induced Fluorescence Transients (NIFTs) have been shown to be a possible way of testing for the limiting nutrient in algal populations. In this study we tested the hypothesis that NIFTs can be used to detect a (co-)limitation for inorganic phosphorus (Pi) and CO 2 in the green alga Chlamydomonas acidophila and that the magnitude of the NIFTs can be related to cellular P:C ratios. We show a co-limitation response for Pi and CO 2 via traditional nutrient enrichment experiments in natural phytoplankton populations dominated by C. acidophila. We measured NIFT responses after a Pi-or a CO 2 -spike in C. acidophila batch cultures at various stages of Pi and inorganic C limitation. Significant NIFTs were observed in response to spikes in both nutrients. The NIFT response to a Pi-spike showed a strong negative correlation with cellular P:C ratio that was pronounced below 3 mmol P: mol C (equivalent to 0.2 pg P cell -1 ). Both cellular P and C content influenced the extent of the Pi-NIFT response. The NIFT response to a CO 2 -spike correlated to low CO 2 culturing conditions and also had a negative correlation with cellular P content. A secondary response within the Pi-NIFT response was related to the CO 2 concentration and potentially reflected co-limitation. In conclusion, NIFTs provided a quick and reliable method to detect the growth-limiting nutrient in an extremophile green alga, under Pi-, CO 2-and Pi/CO 2 (co-)limited growth conditions.

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Section: Effect Of Pmentioning

confidence: 98%

Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga

Spijkerman

Stojkovic

Holland

et al. 2015

European Journal of Phycology

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“…Unlike the relative units from prompt fluorescent methods, the DF technique provides quantitative readings and the integral represents the amount of electrons that at the beginning of photoncounting resided in the photosynthetic apparatus (Strehler and Arnold 1951). Thus, the DFI is affected by both the Chl a concentration and the phytoplankton activity (Istvánovics et al 2005, Berden-Zrimec et al 2010. Due to the downregulation of the number of PSII involved in the lightharvesting process at light saturation, the correlation of the DFI with the Chl a concentration as a biomass parameter for light-adapted algae is limited to the times when the active Chl a concentration is at its daily maximum (Istvánovics et al 2005).…”

Section: Discussionmentioning

confidence: 99%

“…DF instruments were successfully tested for their capability to conduct rapid and highly sensitive biotests based on toxicant-induced changes in the DF decay kinetics of green algae and cyanobacteria (Katsumata et al 2006, Berden-Zrimec et al 2007, Leunert et al 2013. A general overview over the various applications of DF can be found in a book chapter by Berden-Zrimec et al (2010).…”

Section: Introductionmentioning

confidence: 99%

Diurnal changes in the delayed fluorescence response of an ambient light-excited green alga

Eckert¹,

Leunert²,

Yacobi³

et al. 2019

Photosynt.

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Delayed fluorescence (DF) is a characteristic feature of light-excited plant cells caused by the back-reaction of electrons through the electron transport chain. Targeting the response of light-adapted green algae to diurnal light changes, the present study presents novel results of DF measurements in the absence of an artificial excitation light source. Based on a linear relationship between the DF counts and light intensities from 0.15 to 0.65 W m-2 during daybreak, we estimated an initial algal response to light intensities of 0.01 W m-2. Dissolved oxygen concentrations began to increase at 1.0 W m-2. A noon depression similar to that reported for prompt fluorescence occurred above 100 W m-2. Our results from multiple day-night cycles emphasize that the DF response is a function of the chlorophyll concentration and of a rapid light adaptation. The DF counts alone cannot provide a reliable unambiguous measure of photosynthetic activity.

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“…Approximately 0.03% of absorbed solar energy is re-emitted in this manner [ 53 ]. The intensity of the delayed fluorescence emission decays rapidly to undetectable near-background levels over the course of a minute [ 56 , 57 ]; it is therefore critical that images are taken over exactly the same period following lights off in order to observe rhythmicity. This persistence allows the long exposure windows required for detailed imaging.…”

Section: Lumino-fluorescent Circadian Reportersmentioning

confidence: 99%

A comparison of high-throughput techniques for assaying circadian rhythms in plants

et al. 2015

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Over the last two decades, the development of high-throughput techniques has enabled us to probe the plant circadian clock, a key coordinator of vital biological processes, in ways previously impossible. With the circadian clock increasingly implicated in key fitness and signalling pathways, this has opened up new avenues for understanding plant development and signalling. Our tool-kit has been constantly improving through continual development and novel techniques that increase throughput, reduce costs and allow higher resolution on the cellular and subcellular levels. With circadian assays becoming more accessible and relevant than ever to researchers, in this paper we offer a review of the techniques currently available before considering the horizons in circadian investigation at ever higher throughputs and resolutions.

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Delayed Fluorescence

Cited by 17 publications

References 45 publications

Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga

Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga

Diurnal changes in the delayed fluorescence response of an ambient light-excited green alga

A comparison of high-throughput techniques for assaying circadian rhythms in plants

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