Delayed fluorescence, steady state fluorescence, photosystem II quantum yield as endpoints for toxicity evaluation of Cu2+ and Ag+

Strauch, Sebastian M.; Richter, Peter; Haag, Ferdinand W. M.; Krüger, Marcus; Krüger, Julia; Azizullah, Azizullah; Breiter, Roman; Lebert, Michael

doi:10.1016/j.envexpbot.2016.06.005

Cited by 7 publications

(3 citation statements)

References 45 publications

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“…Previous studies on the effects of metal toxicity in Euglena have used a metal exposure period ranging from 1 h to 120 d at a temperature of 20–28 °C and various light conditions ranging from complete darkness to continuous illumination. The associated end-point values were based on chlorophyll a fluorescence, chlorophyll contents, delayed fluorescence, DNA damage, growth, photosynthesis, and reactive oxygen species formation ( Table S1 ; [ 10 , 17 , 19 , 20 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 ]). In this study, the EC 50 values based on velocity and r -value in E. agilis appeared to be within the EC 50 range reported in other end-point studies ( Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

A Ten-Minute Bioassay to Test Metal Toxicity with the Freshwater Flagellate Euglena agilis

Choi

Lee

et al. 2022

Biology

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A chemical analysis of water quality cannot detect some toxicants due to time constraints, high costs, and limited interactions for detection. Bioassays would offer a complementary means to assess pollution levels in water. Euglena is a flagellate green alga and an excellent system for toxicity testing thanks to its ease of culture, rapid growth, and quick response to environmental stresses. Herein, we examined the sensitivity of E. agilis to seven heavy metals by analyzing six end-point parameters: motility, velocity, cell compactness, upward swimming, r-value, and alignment. Notably, the velocity of E. agilis was most sensitive to cadmium (96.28 mg·L−1), copper (6.51 mg·L−1), manganese (103.28 mg·L−1), lead (78.04 mg·L−1), and zinc (101.90 mg·L−1), while r-values were most sensitive to arsenic (12.84 mg·L−1) and mercury (4.26 mg·L−1). In this study, velocity and r-values are presented as useful biomarkers for the assessment of metal toxicity in Euglena. The metals As, Cd, Cu, and Pb were suitable for this test. The advantages of the ecotoxicity test are its rapidity: It takes 10 min to obtain results, as opposed to the typical 3–4 d of exposure time with intensive labor. Moreover, this test can be performed at room temperature under dark conditions.

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Section: Resultsmentioning

confidence: 99%

A Ten-Minute Bioassay to Test Metal Toxicity with the Freshwater Flagellate Euglena agilis

Choi

Lee

et al. 2022

Biology

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“…Significant dose-response of Euglena photosynthesis was reported for copper, zinc, and lead (Danilov & Ekelund, 2001) while cadmium and nickel did not alter photosynthesis in chronic assays (Ahmed & Häder, 2010b). However, photosynthetic parameters of E. gracilis were less sensitive against copper and silver ions as compared to cell motility parameters (Strauch et al, 2016). (Albergoni et al, 1980) reported that copper did not affect the growth rate of E. gracilis while cadmium did, accompanied by a significant intracellular accumulation of the latter.…”

Section: Heavy Metalsmentioning

confidence: 99%

Euglenoid Flagellates in Ecotoxicological Research: Progress and Perspectives

Kottuparambil¹

2022

Preprint

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Euglenoids are a common component of primary producers in high acidic and organic shallow freshwater systems. They are free-living photosynthetic motile flagellates exhibiting rapid responses to various external stimuli including chemical pollution. The unique combination of diverse physiological processes of Euglena such as photosynthesis, movement, and orientation offers a range of sensitive criteria that respond to aquatic pollutants. This has led to the development of several acute and chronic assay methods to monitor the adverse impacts of potent toxicants in aquatic ecosystems. Euglenoids also reflect the consequences of physical stress factors such as UV radiation. This review summarizes the ecotoxicity studies using euglenoid flagellates as model species and their potential to contribute toward rapid screening of ecological impacts of pollution in the aquatic environment. Factors determining the reliability of Euglena tests are outlined.

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“…Moreover, various diseases, such as congestive heart failure, liver infarction, and lung cancer, can occur in the presence of such toxic ions. Some essential metal ions, including Fe 2+ , Cu 2+ , Mn 2+ , and Co 2+ , can also be toxic at higher doses. − In addition, other heavy metal ions, such as Hg 2+ , Cd 2+ , Pb 2+ , and As 3+ , are extremely toxic to humans and aquatic species even at lower concentrations, and the bioaccumulation of these metal ions can culminate in severe health hazards. − …”

Section: Introductionmentioning

confidence: 99%

Gd(III)-Doped Boehmite Nanoparticle: An Emergent Material for the Fluorescent Sensing of Cr(VI) in Wastewater and Live Cells

et al. 2019

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This article reports the effect of Gd(III) doping on the structure, microstructure, and optical properties of boehmite nanoparticles. The bright-blue fluorescence along with a long lifetime makes our material an efficient candidate for optical applications. Our material particularly targets and eliminates hexavalent chromium ions (Cr(VI)) from aqueous media, which turns it into a multifunctional fluorescent nanosensor (MFNS). The development of an efficient hexavalent chromium ion (Cr(VI)) sensor to detect and quantify Cr(VI) ions is still a serious issue worldwide. Thus, this work will be very beneficial for various environmental applications. No such work has been reported so far which includes cost-effective and biocompatible boehmite nanoparticles in this field. Detailed synthesis and characterization procedures for the MFNS have been incorporated here. The biocompatibility of the MFNS has also been studied rigorously by performing cell survivability assay (MTT) and cellular morphology assessments. Our extensive research confirmed that the “turn-off” sensing mechanism of this sensor material is based on a collisional quenching model which initiates the photoinduced electron transfer (PET) process. High selectivity and sensitivity (∼1.05 × 10–5 M) of the MFNS toward hexavalent chromium ions even in real life wastewater samples have been confirmed, which makes this fluorescent probe a potential candidate for new age imaging and sensing technologies.

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Delayed fluorescence, steady state fluorescence, photosystem II quantum yield as endpoints for toxicity evaluation of Cu2+ and Ag+

Cited by 7 publications

References 45 publications

A Ten-Minute Bioassay to Test Metal Toxicity with the Freshwater Flagellate Euglena agilis

A Ten-Minute Bioassay to Test Metal Toxicity with the Freshwater Flagellate Euglena agilis

Euglenoid Flagellates in Ecotoxicological Research: Progress and Perspectives

Gd(III)-Doped Boehmite Nanoparticle: An Emergent Material for the Fluorescent Sensing of Cr(VI) in Wastewater and Live Cells

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