“…Anesthesia was induced by 2% isoflurane mixed with oxygen. Once they lost their reflex of deep pain, the subjects were fixed on the stereotaxic instrument and maintained anesthesia with 1.5% isoflurane 60 . Four tetrodes, consisting of two twisted 0.05 mm stainless steel wires (California Fine Wire, Grover Beach, CA) in each one, were implanted into the NCL (AP, 1.0 mm; ML, − 4.5 mm; DV, 4.0 mm relative to y point), the ENTO (AP, 3.0 mm; ML, − 3.6 mm; DV, 3.3 mm relative to y point), and the ROT (AP, 2.6 mm; ML, − 2.0 mm; DV, 5.0 mm relative to y point), and the VW (AP, 5.5 mm; ML, − 2.0 mm; DV, 1.5 mm relative to y point) in the left hemisphere.…”