Delayed Cutaneous Hypersensitivity Reactions to Autologous Extracts of Burkitt-Lymphoma Cells

Paw Test with Syngeneic Tumor Cells in Inbred Mice Bearing a Transplanted Sarcoma

“…The finding of an inverse correlation between tumor size and cuta neous reactivity to the tumor appears to parallel experience in humans with Burkitt-lymphoma and malignant melanoma [5,6]. Skin reactivity to extracts of autologous tumor was lost in patients with extensive neoplasm.…”

“…Skin reactivity to extracts of autologous tumor was lost in patients with extensive neoplasm. When the tumor mass was reduced by effective therapy, skin reactivity frequently returned [6].…”

Delayed hypersensitivity reactions of cancer patients to antigens on lymphoid cell lines

“…DISCUSSION Skin testing has been shown to be of great value in studying cellular immunity in patients with a number of infectious and non-infectious diseases. Early studies in cancer patients utilizing HMP obtained from fresh cells also had provided promising results, suggesting tumor specificity in two pilot projects, one studying BL (Fass et al, 1970) and another studying identical twins, one with leukemia and one without (Levine et al, 1972). Because of the limited amounts of fresh tumor cells available and the difficulties in standardizing preparations from fresh cells, we began to study tissue-cultured cells as a source of antigen.…”

“…In attempting to define the mechanisms responsible for controlling Epstein-Barr virus (EBV) infections and the subsequent occurrence of malignancies associated with EBV, we have been utilizing a battery of cellular and humoral immunity assays in order to delineate certain aspects of the oncogenic process. In this report, we will describe our results to date with skin testing, an in vivo assay of welldocumented importance in certain infectious diseases (Badger et al, 1963;Dharmendra, 1947) which also is of potential importance in oncologic studies (Fass et al, 1970;Eilber and Morton, 1970;Aisenberg, 1962;Levine et al, 1972;Magrath, 1975).…”

“…Second, the skin test appears to be one of the best immunologic correlates of prognosis. Having been proven to be of extreme clinical importance in certain diseases such as tuberculosis and leprosy, skin testing has been noted to be of prognostic value in patients with melanoma, sarcoma, Burkitt's lymphoma (BL) and a number of other neoplasms (Eilber and Morton, 1970;Fass et al, 1970;Herberman, 1974;Nkrumah et al, 1977). Finally, as a test that is readily standardized and available to countries which do not have sophisticated laboratory technology available for clinical studies, the skin test has allowed us to initiate international collaborative studies in a number of countries interested in EBV immunity.…”