Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders: Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation

Long, Yang; Wang, Jingtao; Wang, Dongmei; Hu, Guojie; Liu, Zi Yuan; Yan, Dongni; Serikuly, Nazar; Alpyshov, Erik T.; Demin, Konstantin A.; Strekalova, Tatyana; Abreu, Murilo S. de; Song, Cai; Kalueff, Allan V.

doi:10.1016/j.bbr.2020.112644

Cited by 24 publications

(18 citation statements)

References 91 publications

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“…Knowing the molecular mechanism of action of the applied antidepressant agent, it is possible to determine its influence on the level of a respective monoamine neurotransmitter in the brain, which can also be affected by exposure to a stressor [ 144 , 145 ]. When analyzing the stress-related behavior in zebrafish, expression levels of pro-inflammatory markers are assessed as well [ 146 , 147 ].…”

Section: Zebrafish In Neurological Studiesmentioning

confidence: 99%

Zebrafish as an Animal Model for Testing Agents with Antidepressant Potential

Lachowicz

Niedziałek

Rostkowska³

et al. 2021

Life

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Depression is a serious mental disease that, according to statistics, affects 320 million people worldwide. Additionally, a current situation related to the COVID-19 pandemic has led to a significant deterioration of mental health in people around the world. So far, rodents have been treated as basic animal models used in studies on this disease, but in recent years, Danio rerio has emerged as a new organism that might serve well in preclinical experiments. Zebrafish have a lot of advantages, such as a quick reproductive cycle, transparent body during the early developmental stages, high genetic and physiological homology to humans, and low costs of maintenance. Here, we discuss the potential of the zebrafish model to be used in behavioral studies focused on testing agents with antidepressant potential.

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Section: Zebrafish In Neurological Studiesmentioning

confidence: 99%

Zebrafish as an Animal Model for Testing Agents with Antidepressant Potential

Lachowicz

Niedziałek

Rostkowska³

et al. 2021

Life

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“…Candidate genes coming from other fish or more generally vertebrate studies with role in memory consolidation could be added (e.g. [112,169] for proposals).…”

Section: Discussionmentioning

confidence: 99%

“…Candidate genes coming from other fish studies with role in memory consolidation could be added (see, e.g. Nyman et al 2020; Yang et al 2020 for proposals).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide epigenomic stress response in the European sea bass (Dicentrarchus labrax, L.)

Krick

Desmarais

Σαμαράς

et al. 2020

Preprint

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AbstractUnderstanding the molecular basis of stress is of long standing interest in biology and fish science. We tackled this question by modifying the epiGBS (epiGenotyping By sequencing) technique to screen for cytosine methylation and explore the genome-wide epigenomic response to a repeated acute stress challenge in the European sea bass (Dicentrarchus labrax). Following a minimally invasive sampling using nucleated red blood cells (RBCs), our modified epiGBS protocol retrieved 501,108,033 sequencing reads after trimming, with a mean mapping efficiency of 73.0% (unique best hits). Sequencing reads mapped across all linkage groups (LGs). A total of 47,983 CpG coordinates with a minimum 30X read depth was retained for differential methylation analysis between pre- and post-stress fish. A family effect was demonstrated, and 57 distinct differentially methylated cytosines (DMCs) distributed on 17 of 24 LGs were found between RBCs of pre- and post-stress individuals and located close to 51 distinct stress-related genes. Thirty-eight of these genes were previously reported as differentially expressed in the brain of zebrafish, most of them involved in stress coping differences. Some DMC-related genes appear as good candidates to study the stress response, especially a set of them associated to the Brain Derived Neurotrophic Factor (BDNF), a protein that favors stress adaptation and fear memory. Limits to our study and future directions are presented, including the use of RBCs as a surrogate to other target tissues, to provide with classical physiological measurements a more complete picture of the stress response in fish.

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“…Reaction (22.5 µl total volumes) contained 4.5 µl of water, 10 µl of 2 x SYBR green mixes, 2 µl of 10 µM forward and reverse primer of a gene, and 4 µl cDNA. The following 3 step qRT-PCR reaction was performed: Pre-denaturation at 95°C for 30 s, followed by 35 cycles of denaturation at 95°C for 5 min, annealing at 60°C for 30 s, and elongation at 72°C for 20 s. The expression levels of the cytokine genes relative to β -actin were calculated by the comparative Ct method (2-ΔΔCt) [19,20].…”

Section: Step 3: Real-time Polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Evaluation of immunomodulatory activity of Immusante® in zebrafish

Agraz¹,

Rafiq²,

Guru³

et al. 2021

J App Biol Biotech

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Immusante ® (IM-133N) a proven polyherbal immunomodulator was evaluated for its immunomodulatory activity in zebrafish models. In the present study, Aeromonas hydrophila (A. hydrophila) is used to develop the experimental model for studying immunomodulatory activity in zebrafish. To determine the A. hydrophila dose for the study, either sex of 30 zebrafish were taken and divided into 5 groups followed by intra-peritoneal injected with 10 µl of 6.0 × 10 6 , 6.0 × 20 5 , 6.0 × 10 5 , 6.0 × 10 4 , and 6.0 × 10 3 cells/ml of live A. hydrophila suspension, respectively and continuously cultured. Acute toxicity study of Immusante ® was done as per the procedures described in OECD 203 guideline. To examine the protective role of Immusante ® and to check the survival rate, two zebrafish survival assays were performed. Immusante ® was evaluated on A. hydrophila infection in zebrafish to study the effect and differential WBC count was also measured in the zebrafish infected with A. hydrophila. Gene expression analysis was done to check the expression levels of pro-inflammation cytokines. Immusante ® at a dose of 200 and 400 mg/L enhanced the survival rate of infected zebrafish and at 400 mg/L, decreased the number of colony forming units (CFUs) of A. hydrophila in the blood and kidney of infected fishes. A significant increase in lymphocyte and decrease in neutrophils was observed in the treatment groups, which are the signs of improvement in non-specific immune response. Immusante ® at a dose of 400 mg/L suppressed the pro-inflammatory cytokine's expression such as IL-1β, IFN-γ, and TNF-α in the kidney of infected zebrafish and prevented the detrimental effects on zebrafish caused due to excessive inflammatory response.

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Delayed behavioral and genomic responses to acute combined stress in zebrafish, potentially relevant to PTSD and other stress-related disorders: Focus on neuroglia, neuroinflammation, apoptosis and epigenetic modulation

Cited by 24 publications

References 91 publications

Zebrafish as an Animal Model for Testing Agents with Antidepressant Potential

Zebrafish as an Animal Model for Testing Agents with Antidepressant Potential

Genome-wide epigenomic stress response in the European sea bass (Dicentrarchus labrax, L.)

Evaluation of immunomodulatory activity of Immusante® in zebrafish

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