Delayed bactericidal activity of beta-lactam antibiotics against Listeria monocytogenes: antagonism of chloramphenicol and rifampin

Winslow, Dean L.; Damme, J; Dieckman, E

doi:10.1128/aac.23.4.555

Cited by 60 publications

(34 citation statements)

References 25 publications

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“…However, the combination of beta-lactam antibiotics and rifampin has previously been shown to result in an antagonistic relationship in some bacteria, where the bactericidal activity of the beta-lactam is decreased by the presence of rifampin (10,36). The antagonistic relationship of this antibiotic combination was reinforced further by the biofilm data.…”

Section: Discussionsupporting

confidence: 56%

Biofilm Formation by Group A Streptococci: Is There a Relationship with Treatment Failure?

Conley¹,

Olson²,

Cook³

et al. 2003

J Clin Microbiol

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Group A streptococcus (GAS) is the primary cause of bacterial pharyngitis in children and adults. Up to one-third of patients treated for GAS pharyngitis fail to respond to antibiotic therapy. The objective of this cohort study was to evaluate GAS biofilm formation as a mechanism for antibiotic treatment failure using previously collected GAS isolates and penicillin treatment outcome data. The minimum biofilm eradication concentration (MBEC) assay device was used to determine the biofilm-forming capabilities, efficiencies, and antibiotic susceptibilities of GAS isolates. The MBECs and MICs of several antibiotics for GAS were determined. All 99 GAS isolates available for this study formed biofilms, with various efficiencies. Antibiotic MBECs were consistently higher than MICs for all of the GAS isolates. MBECs indicated penicillin insensitivity in 60% of GAS isolates, producing the first report of in vitro GAS insensitivity to penicillin. Using MBECs to predict penicillin treatment failure had better sensitivity (56%) but lower specificity (36%) than the sensitivity (0%) and specificity (100%) when MICs were used. However, the positive predictive value of the MBEC was superior to that of the MIC (56 versus 0%), while the negative predictive values (42 and 47%) were similar. More studies are needed to understand the roles of biofilms and the MBEC assay in predicting GAS treatment failure. In addition, further investigations are necessary to determine if non-biofilm-forming strains of GAS exist and the roles of in vivo monospecies and multispecies biofilms in streptococcal pharyngitis treatment failure.

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Section: Discussionsupporting

confidence: 56%

Biofilm Formation by Group A Streptococci: Is There a Relationship with Treatment Failure?

Conley¹,

Olson²,

Cook³

et al. 2003

J Clin Microbiol

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“…However, in a previous study we observed a biphasic killing pattern when L. monocytogenes was exposed to increasing concentrations of norfloxacin and gentamicin (34). A similar biphasic killing patterns can be found in older literature when the bacterium was treated with bactericidal antibiotics (35), although some reports also demonstrate a complete killing of L. monocytogenes by bactericidal antibiotics (36,37).…”

mentioning

confidence: 74%

Survival of Bactericidal Antibiotic Treatment by a Persister Subpopulation of Listeria monocytogenes

Knudsen

Gram

2013

Appl Environ Microbiol

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Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, which are a dormant antibiotic-tolerant subpopulation. The purpose of this study was to determine whether L. monocytogenes can form persisters and how bacterial physiology affects the number of persisters in the population. A stationary-phase culture of L. monocytogenes was adjusted to 10 8 CFU ml ؊1, and 10 3 to 10 4 CFU ml ؊1 survived 72-h treatment with 100 g of norfloxacin ml ؊1 , indicating a persister subpopulation. This survival was not caused by antibiotic resistance as regrown persisters were as sensitive to norfloxacin as the parental strain. Higher numbers of persisters (10 5 to 10 6 ) were surviving when older stationary phase or surface-associated cells were treated with 100 g of norfloxacin ml ؊1. The number of persisters was similar when a ⌬sigB mutant and the wild type were treated with norfloxacin, but the killing rate was higher in the ⌬sigB mutant. Dormant norfloxacin persisters could be activated by the addition of fermentable carbohydrates and subsequently killed by gentamicin; however, a stable surviving subpopulation of 10 3 CFU ml ؊1 remained. Nitrofurantoin that has a growth-independent mode of action was effective against both growing and dormant cells, suggesting that eradication of persisters is possible. Our study adds L. monocytogenes to the list of bacterial species capable of surviving bactericidal antibiotics in a dormant stage, and this persister phenomenon should be borne in mind when developing treatment regimens.

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“…In view of the high rate of clinical failures observed with the reference treatment, alternative treatments have been proposed, and these alternative treatments have been based on the high degree of intracellular penetration of certain antibiotics. The value of rifampin, certain fluoroquinolones, and even chloramphenicol, alone or in combination, has been emphasized (30). Co-trimoxazole has been proposed for use in patients who are hypersensitive to beta-lactam antibiotics and has also been recommended as first-line treatment because of the good diffusion of sulfame-thoxazole and trimethoprim into the meninges (22,26).…”

mentioning

confidence: 99%

Inhibition of intracellular growth of Listeria monocytogenes by antibiotics

Michelet¹,

Avril²,

Cartier³

et al. 1994

Antimicrob Agents Chemother

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We studied the activities of 15 antibiotics on the intracellular growth of Listeria monocytogenes in a HeLa cell line. After 24 h of contact with the infected cells, the antibiotics most effective against the intracellular growth of the 10 strains tested were amoxicillin, temafloxacin, and sparfloxacin, which nevertheless failed to totally eliminate the intracellular bacteria. Rifampin and co-trimoxazole had variable effects, depending on the isolates studied. The most active combinations were amoxicillin-sparfloxacin, co-trimoxazole-gentamicin, and sparfloxacin-co-trimoxazole. The results suggest the value of using a cell culture technique to study the activities of antibiotics against certain bacteria with intracellular sites of multiplication.

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Delayed bactericidal activity of beta-lactam antibiotics against Listeria monocytogenes: antagonism of chloramphenicol and rifampin

Cited by 60 publications

References 25 publications

Biofilm Formation by Group A Streptococci: Is There a Relationship with Treatment Failure?

Biofilm Formation by Group A Streptococci: Is There a Relationship with Treatment Failure?

Survival of Bactericidal Antibiotic Treatment by a Persister Subpopulation of Listeria monocytogenes

Inhibition of intracellular growth of Listeria monocytogenes by antibiotics

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