“…We believe that coupling scRNA-Seq with scTCR-Seq from both skin and blood is fundamental when studying skin diseases, as the skin hosts more memory T cells compared with the circulation ( Clark, 2015 ). The identification of T cell receptor via scTCR-Seq allows tracking of specific TCR clones between skin and blood to analyze their phenotype and distribution in tissue ( Saluzzo et al., 2021 ). We provide evidence of the suitability of this protocol to study structural cells in the human skin and suggest statistical tools that can be used for quantification and bioinformatics analysis of their interaction with immune cells.…”