Delayed antiretroviral therapy in HIV-infected individuals leads to irreversible depletion of skin- and mucosa-resident memory T cells

Processing human skin samples for single-cell assays

“…Right: example of cell identity assignments for skin and blood cells. Figure reprinted with permission from Saluzzo et al. (2021) .…”

“…We believe that coupling scRNA-Seq with scTCR-Seq from both skin and blood is fundamental when studying skin diseases, as the skin hosts more memory T cells compared with the circulation ( Clark, 2015 ). The identification of T cell receptor via scTCR-Seq allows tracking of specific TCR clones between skin and blood to analyze their phenotype and distribution in tissue ( Saluzzo et al., 2021 ). We provide evidence of the suitability of this protocol to study structural cells in the human skin and suggest statistical tools that can be used for quantification and bioinformatics analysis of their interaction with immune cells.…”

“…We provide evidence of the suitability of this protocol to study structural cells in the human skin and suggest statistical tools that can be used for quantification and bioinformatics analysis of their interaction with immune cells. This is an improved protocol, modified from Saluzzo et al. (2021) .…”

“…We provide an example in which we coupled single-cell RNA sequencing (scRNA-seq) with single-cell T-cell receptor sequencing (scTCR-seq) of skin and blood cells to study transcriptional profiles and clonotypes of skin resident and peripheral circulating, memory, and effector T cells. This is an improved protocol based on Saluzzo et al. (2021) .…”

“…(2021) . For complete details on the use and execution of this protocol, please refer to Saluzzo et al. (2021) .…”