Delay in flowering and increase in biomass of transgenic tobacco expressing the Arabidopsis floral repressor gene FLOWERING LOCUS C

Salehi, H.; Ransom, Callista; Oraby, Hesham F.; Seddighi, Zahra; Sticklen, Mariam B.

doi:10.1016/j.jplph.2004.12.002

Cited by 54 publications

(25 citation statements)

References 24 publications

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“…Studies in Arabidopsis and rice show that they are also involved in modulating developmental phase changes. Delayed flowering (reproductive growth) and prolonged vegetative phase greatly improve biomass production (Salehi et al 2005). These suggest that expression of the BdGI or its orthologues can be modified to engineer flowering initiation and phase transitions in Brachypodium and other grass crop species with an aim of improving biomass production.…”

Section: Discussionmentioning

confidence: 99%

Identification and molecular characterization of a Brachypodium distachyon GIGANTEA gene: functional conservation in monocot and dicot plants

et al. 2009

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Developmental phase change and flowering transition are emerging as potential targets for biomass agriculture in recent years. The GIGANTEA (GI) gene is one of the central regulators that direct flowering promotion and phase transition. In this work, we isolated a GI gene orthologue from the small annual grass Brachypodium distachyon inbred line Bd21 (Brachypodium), which is perceived as a potential model monocot for studies on bioenergy grass species. A partial GI gene sequence was identified from a Brachypodium expressed sequence tag library, and a full-size gene (BdGI) was amplified from a Brachypodium cDNA library using specific primer sets designed through analysis of monocot GI gene sequences. The BdGI gene was up-regulated by light and cold. A circadian rhythm set by light-dark transition also regulated the expression of the BdGI gene. The deduced amino acid sequence of the BdGI protein shares higher than 70% of sequence identity with the GI proteins in monocots and Arabidopsis. In addition, the BdGI protein is constitutively targeted to the nucleus and physically interacts with the ZEITLUPE (ZTL) and CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) proteins, like the Arabidopsis GI protein. Interestingly, heterologous expression of the BdGI gene in a GI-deficient Arabidopsis mutant rescued efficiently the late flowering phenotype. Together, our data indicate that the role of the GI gene in flowering induction is conserved in Arabidopsis and Brachypodium. It is envisioned that the GI genes of bioenergy grasses as well as Brachypodium could be manipulated to improve biomass by engineering developmental timing of phase transitions.

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Section: Discussionmentioning

confidence: 99%

Identification and molecular characterization of a Brachypodium distachyon GIGANTEA gene: functional conservation in monocot and dicot plants

et al. 2009

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“…of fi cinarum ( SoLFY ) in antisense orientation was suf fi cient to suppress fl owering in some of the transgenic sugarcane lines tested under fi eld conditions (Ulian 2006 ) . Other studies in tobacco have shown that expression of the Arabidopsis fl ower repressor, FLOWERING LOCUS C , enhanced biomass production (Salehi et al 2005 ) . Transgenic sugarcane lines with impaired fl owering and high biomass would be a valuable source of cellulosic biofuel.…”

Section: Modulation Of Floweringmentioning

confidence: 96%

Genetic Engineering of Saccharum

Beyene

Curtis

Damaj

et al. 2012

Genomics of the Saccharinae

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Over the last two decades, substantial progress has been made in the genetic engineering of sugarcane ( Saccharum spp.) through improvements in tissue culture procedures, allowing a higher ef fi ciency of generating transgenic plants using Agrobacterium -mediated and biolistic gene transfers. Elucidation of gene function and development of varieties with improved yield, sugar level, fi ber content, and other desirable traits and products for superior performance have been possible through transgenic technologies. Researchers are now focusing on optimizing existing methodologies and developing new technologies for the production of elite varieties, enhancement of transgene expression, and manipulation of metabolic pathways for improved molecular breeding and commercial exploitation. At present, no transgenic sugarcane has been released to the commercial market, but with the aid of large investments from the private sector, the commercialization of this major sugar-and biomass-producing crop should be accelerated.

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“…Therefore, FLC counteracts FT activation by other factors such as CO (Bäurle and Dean 2006) and possibly also FPF1. In another study, transgenic plants expressing the Arabidopsis FLOWERING LOCUS C gene showed delayed flowering and increased biomass production (Salehi et al 2005). In addition, PtFT1 is not only an efficient regulator of FTi but also a suppressor of short-dayinduced growth cessation and bud set in poplar.…”

Section: Links Between Fti Gene Expression and Other Developmental Prmentioning

confidence: 99%

Influence of over-expression of the FLOWERING PROMOTING FACTOR 1 gene (FPF1) from Arabidopsis on wood formation in hybrid poplar (Populus tremula L. × P. tremuloides Michx.)

Hoenicka

Lautner

Klingberg

et al. 2011

Planta

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Constitutive expression of the FPF1 gene in hybrid aspen (Populus tremula L. × P. tremuloides Michx.) showed a strong effect on wood formation but no effect on flowering time. Gene expression studies showed that activity of flowering time genes PtFT1, PtCO2, and PtFUL was not increased in FPF1 transgenic plants. However, the SOC1/TM3 class gene PTM5, which has been related to wood formation and flowering time, showed a strong activity in stems of all transgenic lines studied. Wood density was lower in transgenic plants, despite significantly reduced vessel frequency which was overcompensated by thinner fibre cell walls. Chemical screening of the wood by pyrolysis GC/MS showed that FPF1 transgenics have higher fractions of cellulose and glucomannan products as well as lower lignin content. The latter observation was confirmed by UV microspectrophotometry on a cellular level. Topochemical lignin distribution revealed a slower increase of lignin incorporation in the developing xylem of the transgenics when compared with the wild-type plants. In line with the reduced wood density, micromechanical wood properties such as stiffness and ultimate stress were also significantly reduced in all transgenic lines. Thus, we provide evidence that FPF1 class genes may play a regulatory role in both wood formation and flowering in poplar.

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Delay in flowering and increase in biomass of transgenic tobacco expressing the Arabidopsis floral repressor gene FLOWERING LOCUS C

Cited by 54 publications

References 24 publications

Identification and molecular characterization of a Brachypodium distachyon GIGANTEA gene: functional conservation in monocot and dicot plants

Identification and molecular characterization of a Brachypodium distachyon GIGANTEA gene: functional conservation in monocot and dicot plants

Genetic Engineering of Saccharum

Influence of over-expression of the FLOWERING PROMOTING FACTOR 1 gene (FPF1) from Arabidopsis on wood formation in hybrid poplar (Populus tremula L. × P. tremuloides Michx.)

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