Del-1 Plays a Protective Role against COPD Development by Inhibiting Inflammation and Apoptosis

Abstract

Neutrophilic inflammation is a prominent feature of chronic obstructive pulmonary disease (COPD). Developmental endothelial locus-1 (Del-1) has been reported to limit excessive neutrophilic inflammation by inhibiting neutrophil adhesion to the vascular endothelial cells. However, the effects of Del-1 in COPD are not known. We investigated the role of Del-1 in the pathogenesis of COPD. Del-1 protein expression was decreased in the lungs of COPD patients, especially in epithelial cells and alveolar macrophages. In contrast to human lung tissue, Del-1 expression was upregulated in lung tissue from mice treated with cigarette smoke extracts (CSE). Overexpression of Del-1 significantly suppressed IL-8 release and apoptosis in CSE-treated epithelial cells. In contrast, knockdown of Del-1 enhanced IL-8 release and apoptosis. In macrophages, overexpression of Del-1 significantly suppressed inflammatory cytokine release, and knockdown of Del-1 enhanced it. This anti-inflammatory effect was mediated by inhibiting the phosphorylation and acetylation of NF-κB p65. Nuclear factor erythroid 2-related factor 2 (Nrf2) activators, such as quercetin, resveratrol, and sulforaphane, increased Del-1 in both cell types. These results suggest that Del-1, mediated by Nrf2, plays a protective role against the pathogenesis of COPD, at least in part through anti-inflammatory and anti-apoptotic effects.

3-methyladenine ameliorates acute lung injury by inhibiting oxidative damage and apoptosis

“…Proinflammatory factors lead to apoptosis in lung epithelial and endothelial cells [ 20 ], whereas activation of the Nrf2 pathway attenuates cell apoptosis [ 21 ]. Nrf2 also has anti-inflammatory and antiapoptotic properties in chronic obstructive pulmonary disease [ 22 ]. However, whether 3-MA can alleviate ALI by modulating the levels of apoptosis through the PKCα and Nrf2 pathways has not been fully elucidated.…”