Deinococcus cellulosilyticus sp. nov., isolated from air

Abstract

A pink-coloured bacterial strain, 5516J-15 T , was isolated from an air sample from Jeju Island, Republic of Korea. The organism was found to have resistance to UV radiation typical of members of the genus Deinococcus, and it was placed within the radiation of the Deinococcus on a phylogenetic tree based on 16S rRNA gene sequences. Strain 5516J-15 T shared low 16S rRNA gene sequence similarity (84.5-87.8 %) with Deinococcus species, showing highest sequence similarity to Deinococcus deserti VCD115 T (87.8 %) and Deinococcus indicus Wt/1a T (87.8 %).Strain 5516J-15 T had type A3b peptidoglycan with L-ornithine, menaquinone 8 (MK-8) as the major quinone and iso-C 12 : 0 , anteiso-C 13 : 0 , iso-C 16 : 0 and C 16 : 0 as the major fatty acids. Its polar lipid profile contained three unknown aminophospholipids, two unknown polar lipids, one unknown phospholipid and one unknown glycolipid. The DNA G+C content of strain 5516J-15 T was 61.3 mol%. Based on the phylogenetic and phenotypic data presented, it is proposed that the unknown strain should be classified within a novel species in the genus Deinococcus with the name Deinococcus cellulosilyticus sp. nov. The type strain is 5516J-15 T (5KACC 11606Since the first report of the genus Deinococcus (Brooks & Murray, 1981), 23 species with validly published names have been recovered from various environments such as soils, hot springs, foods, faeces, air dust and the rhizosphere. The most prominent characteristic of this genus is extreme resistance to UV and gamma radiation and desiccation. In the course of the study of the bacterial population from air samples, we isolated a novel Deinococcus isolate. Here, we report on the taxonomic characterization of pink-coloured strain 5516J-15T .An air sample was collected in the Jeju region using an MAS-100 air sampler (Merck) (single-stage multiple-hole impactor). The sampler contained a Petri dish with R2A agar (BBL) amended with 200 mg cycloheximide ml 21 (Sigma). After sampling, the plate was incubated at 28 u C for 5 days, and strain 5516J-15 T was recovered.Sequencing of the 16S rRNA gene was performed as described previously (Kwon et al., 2003). Sequence comparison between isolate 5516J-15 T and sequences from Deinococcus species with validly published names obtained from GenBank was conducted by using CLUSTAL W software (Thompson et al., 1994). Strain 5516J-15 T showed low 16S rRNA gene sequence similarity (84.5-87.8 %) to Deinococcus species, showing highest sequence similarity to Deinococcus deserti VCD115 T (87.8 %) and Deinococcus indicus Wt/1a T (87.8 %). Phylogenetic analyses were performed using the MEGA 3 program (Kumar et al., 2004). Phylogenetic dendrograms were constructed using the neighbour-joining and maximum-parsimony methods with bootstrap values based on 1000 replications. According to the neighbour-joining tree ( Fig. 1), strain 5516J-6 T was closely related to the genus Deinococcus with the support of a high bootstrap value (100 %) despite beingThe GenBank accession number for the 16S rRNA gene sequenc...

UV-resistant bacteria isolated from upper troposphere and lower stratosphere

“…The airborne Deinococcus was firstly found from hospital air contaminant (strain SARK) (Murray and Robinow, 1958), and isolated later from indoor air dust (Christensen and Kristensen, 1981) and recently from island surface air (Weon et al, 2007). The two Deinococcus strains isolated in the current study are the first documentation of the genus Deinococcus from upper boundary of the biosphere, the upper atmosphere.…”

“…The D-values were calculated from the regression line of the exponential slope of the survival curves as described in Arrage et al (1993a 2 and TR1103-3 exhibited comparable UV-resistance to the most UV-resistant isolate from surface atmosphere. Deinococcus-related isolates (ST0316 and TR0125) exhibited extreme UV resistance common to described Deinococcus strains (Weon et al, 2007). The high UVresistant isolate TR0126 is a Streptomyces-related strain.…”

“…The isolates of the genus Deinococcus have been described from diverse surface habitats (Weon et al, 2007) to the deep-sea biosphere (Kimura et al, 2003). The airborne Deinococcus was firstly found from hospital air contaminant (strain SARK) (Murray and Robinow, 1958), and isolated later from indoor air dust (Christensen and Kristensen, 1981) and recently from island surface air (Weon et al, 2007).…”

Characterization of Deinococcus sahariens sp. nov., a radiation-resistant bacterium isolated from a Saharan hot spring

“…Currently, a large number of studies are being carried out on Deinococcus species because of their high resistance to ionizing radiation. These species have been isolated from a wide range of environments, e.g., desert soil (De Groot et al 2005;Rainey et al 2007;Peng et al 2009), air (Yeon et al 2007;Yoo et al 2010), marine Wsh (Shashidharand and Bandekar 2009), aquifers (Suresh et al 2004), plant rhizosphere (Lai et al 2006), hot springs (Ferreira et al 1997), high atmosphere (Yang et al 2009) and stratosphere (Yang et al 2010). The majority of Deinococcus species are reported to have optimal growth temperatures in the mesophilic range.…”

Complete genome sequence of Deinococcus maricopensis type strain (LB-34T)

“…In conclusion, an emended description of the genus Deinococcus was published, showing that the cells can be spherical or rod-shaped [4]. Members of the genus Deinococcus were isolated from various environmental habitats including air [5-7], arid soil [1,8-12], water and activated sludge [13-15], alpine environments [16], rhizosphere [17], Antarctica [18], hot springs [19], aquifer [20], marine fish [21] and radioactive sites [22]. Here we present a summary classification and a set of features for D. maricopensis LB-34 T , together with the description of the complete genomic sequencing and annotation.…”