Dehydrozaluzanin C, a natural sesquiterpenolide, causes rapid plasma membrane leakage

Galindo, Juan C. G.; Hernández, A.; Dayan, Franck E.; Tellez, Mario R.; Macı́as, Francisco A.; Paul, Rex N.; Duke, Stephen O.

doi:10.1016/s0031-9422(99)00303-9

Cited by 94 publications

(58 citation statements)

References 31 publications

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“…4). This observation is in agreement with that obtained with cucumber cotyledons treated with natural sesquiterpenolide, dehydrozaluzanin C, causing a dramatic and rapid plasma membrane leakage (Galindo et al, 1999). In the present paper, symptoms of membrane injuries occurred concomitantly to H 2 O 2 accumulation (Fig.…”

Section: Discussionsupporting

confidence: 93%

“…They include disruption of membrane permeability (Galindo et al, 1999), ion uptake (Lehman and Blum, 1999), inhibition of electron transport in both photosyntetic and the respiratory chain (Peñuelas et al, 1996;Abrahim et al, 2000;Czarnota et al, 2001), alterations of enzyme activities (Cruz-Ortega et al, 1990;Politycka, 1998), and inhibition of cell division (Cruz-Ortega et al, 1988;Anaya and Pelayo-Benavides, 1997). It has also been postulated that allelopathy stress might lead to an imbalance between antioxidant defenses and the amount of reactive oxygen species (ROS), resulting in oxidative stress (Cruz-Ortega et al, 2002;Bais et al, 2003;Romero-Romero et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Induction of Oxidative Stress by Sunflower Phytotoxins in Germinating Mustard Seeds

et al. 2007

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The aim of this study was to investigate the phytotoxic effect of sunflower on physiological and biochemical processes during germination of mustard seeds (Sinapis alba L. cv. Nakielska). To exclude the involvement of osmotic stress in seed reaction to phytotoxic compounds, we compared the effect of 10% (w/v) water extract from sunflower (Helianthus annuus L. cv. Ogrodowy) leaves and 28.4% (w/v) polyethylene glycol (PEG) 8000 solution characterized by an equal Y =−1 MPa. We evaluated (1) the amount of hydrogen peroxide (H 2 O 2 ); (2) activities of antioxidant enzymes: superoxide dismutase, catalase, and glutathione reductase; (3) membrane permeability; and (4) level of malondialdehyde (MDA). Both, sunflower compounds and PEG solutions inhibited mustard seed germination, but only phytotoxins caused an increase in the cell membrane permeability, MDA level, H 2 O 2 concentration, and alterations in activities of antioxidant enzymes. Our results demonstrate that despite the activation of the antioxidant system by sunflower phytotoxins, reactive oxygen species accumulation caused cellular damage, which resulted in the decrease of germinability and gradual loss of seed vigor. It seems that the negative effect of sunflower on germination of mustard seeds is mostly because of its toxicity and not to its contribution to osmotic potential.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Induction of Oxidative Stress by Sunflower Phytotoxins in Germinating Mustard Seeds

et al. 2007

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“…The electric conductivities of E. coli suspensions treated with orange pigment increased significantly (P < 0.05) from 100.6 to 231.0 µS/cm with treatment time (0-9 h). The increase in the electric conductivity of the cell suspensions suggested that the cytoplasmic membranes were disrupted, which caused cellular leakage (Dayan et al 1999;Galindo et al 1999). The disruption of bacterial membrane was probably caused by the interaction between orange pigment and the phospholipid of cytoplasmic membranes.…”

Section: Resultsmentioning

confidence: 99%

Antibacterial characteristics of orange pigment extracted from Monascus pigments against Escherichia coli

Zhao¹,

Li²,

Yang³

et al. 2016

Czech J. Food Sci.

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Zhao G.P., Li Y.Q., Yang J., Cui K.Y. (2015): Antibacterial characteristics of orange pigment extracted from Monascus pigments against Escherichia coli. Czech J. Food Sci.,.The antibacterial characteristics of orange pigment, which is one of the Monascus pigments, against Escherichia coli were investigated. Orange pigment exhibited strong antibacterial activity against E. coli evidenced by an increase in the diameter of inhibition zone with orange pigment treatment. The concentration of 2.5 mg/ml was the minimum inhibitory concentration of orange pigment against E. coli. Scanning electron microscopy revealed that orange pigment could damage bacterial cells, eventually resulting in cell death. The increase in the electric conductivity of bacterial cell suspensions suggested that the cytoplasmic membrane was broken by treatment with orange pigment. The result of orange pigment incorporation into egg PC further demonstrated the interaction between orange pigment and the phospholipid led to the disruption of bacterial membrane.

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“…Each research team develops their own approach that best addresses their needs, and teams often rely on methods with which they are most comfortable. Dehydrozaluzanin C, a natural sesquiterpene lactone, causes rapid plasma membrane leakage in cucumber cotyledon disks (9). The essential oil extracted from palmarosa (Cymbopogon martinii) causes the proportion of membrane fatty acids to change (33).…”

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confidence: 99%

Isolation and Characterization of a Novel Antialgal Allelochemical from Phragmites communis

2005

Appl Environ Microbiol

185

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Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC 50 ) of the allelopathic fractions on C. pyrenoidosa and M. aeruginosa were 0.49 and 0.79 mg/liter, respectively. The allelopathic activity of the fraction was species-specific. The isolated allelopathic fraction caused metal ion leakage from algal cells. The fraction decreased the activities of antioxidant enzymes, such as superoxide dismutase and peroxidase. The addition of the isolated fraction increased the concentration of unsaturated lipid fatty acids in cell membrane of C. pyrenoidosa and M. aeruginosa. This caused a change in plasma membrane integrity and the leakage of ions in the protoplast. The allelopathic compound was identified by nuclear magnetic resonance and gas chromatography-mass spectrometry as ethyl 2-methylacetoacetate. Synthesized ethyl 2-methylacetoacetate also showed allelopathic activity on C. pyrenoidosa and M. aeruginosa. The EC 50 of synthesized ethyl 2-methylacetoacetate on C. pyrenoidosa and M. aeruginosa were 0.49 and 0.65 mg/liter, respectively.

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Dehydrozaluzanin C, a natural sesquiterpenolide, causes rapid plasma membrane leakage

Cited by 94 publications

References 31 publications

Induction of Oxidative Stress by Sunflower Phytotoxins in Germinating Mustard Seeds

Induction of Oxidative Stress by Sunflower Phytotoxins in Germinating Mustard Seeds

Antibacterial characteristics of orange pigment extracted from Monascus pigments against Escherichia coli

Isolation and Characterization of a Novel Antialgal Allelochemical from Phragmites communis

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