Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay

Metcalf, Mary G.

doi:10.1016/0039-128x(76)90042-8

Cited by 32 publications

(6 citation statements)

References 8 publications

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“…Serum T and DHEA-S were measured by radioimmunoassay with previously published procedures (Furuyama et al, 1970;Metcalf, 1976), and serum A by radioimmunoassay after column chromatography (Endocrine Sciences Androstenedione Procedure Manual, 1972). SHBG was determined by a saturation technique similar to that described by Rosner (1972).…”

Section: Methodsmentioning

confidence: 99%

Serum androgens and sex hormone binding globulin in obese Pima Indian females

Purifoy

Koopmans

Tatum

et al. 1981

American J Phys Anthropol

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Levels of serum androgens and sex hormone binding globulin (SHBG) were measured in 20 obese Pima Indian females aged 19-44 and compared with those of normal-weight Caucasians aged 20-46. The Pima exhibited significantly decreased SHBG compared to Caucasians, but a strong effect of age on androgen levels rendered mean comparisons useless. Androstenedione (A) and dehydroepiandrosterone-sulfate (DHEA-S) decreased significantly, and testosterone (T) declined slightly with age in the Pima, whereas these androgens showed no significant decreases in Caucasians for this age range. A possible relationship of androgens to the Pima female's propensity for android obesity as well as possible effects of obesity on SHBG, and aging is discussed.

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Section: Methodsmentioning

confidence: 99%

Serum androgens and sex hormone binding globulin in obese Pima Indian females

Purifoy

Koopmans

Tatum

et al. 1981

American J Phys Anthropol

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“…Dehydroepiandrosterone sulphate in peripheral serum (S-DHAS) was determined by a modification of the procedure of Metcalf (24). The method includes dilution of the serum 1 : 10 000 with 0.04 M sodium acetate buffer pH 4.…”

Section: Months Of Therapymentioning

confidence: 99%

Treatment with oral estrone sulphate in the female climacteric: II. Hormonal aspects

Lagrelius

Carlström

Lunell

1981

Acta Obstet Gynecol Scand

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Of forty-three perimenopausal women aged 40.1-56.5 years with climacteric complaints 25 were treated with sodium piperazine estrone sulphate 2.5 mg daily and 18 were given methyl scopolamine. All subject had initial serum FSH levels in the postmenopausal range (20 U/l). The serum levels of FSH, LH, unconjugated immunoreactive estrogens, total estrone, total dehydroepiandrosterone (DHAS), and the urinary low polar estrogens were followed during 18 months of treatment. In the estrogen treated group serum unconjugated immunoreactive estrogens and total estrone as well as urinary low polar estrogens increased significantly during treatment. The serum levels of FSH decreased significantly during the whole test period; LH only at three months. No effects could be seen on serum DHAS. In the group given methyl scopolamine the only changes found were a decrease in FSH at three months and a positive trend for LH (during the whole treatment period). In the total material, positive correlations were found between pretreatment values of unconjugated immunoreactive estrogens and total estrone and between total estrone and DHAS. A negative correlation was found between unconjugated immunoreactive estrogens and FSH. It is concluded that the effects of estrogens on the endocrine system in peri- and postmenopausal women are influenced by chemical structure, dosage and mode of administration of the estrogen as well as by the endogenous hormone profile.

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“…The concentration in serum of most adrenal androgens declines sharply after the age of 40 and after 60 years the level of the main adrenal androgen DHAS corresponds to only 20% of its maximum value at 20-40 years (24,63,86,110). In contrast, the cortisol levels remain stable throughout life (107).…”

Section: Acta Obster Gynecol Scand Suppl107mentioning

confidence: 99%

“…Intra-and inter-assay variations were 8.0 and 10.5% for FSH, 8.7 and 8.7% for LH, 7.2 and 14.6% for prolactin, 11.7 and 13.1% for estradiol-17P and 7.0 and 8.9% for total estrone. Serum DHAS was measured by a modification of Metcalf's procedure (16,63). The method includes hydrolysis of DHAS by autoclaving diluted serum at pH 4.5 at 120"C, extraction with diethyl ether and radioimmunoassay using anti-dehydro-epiandrosterone-1 7-(carboxymethyl oxime) bovine serum albumin (Hypolab S.A., Coinsins, Switzerland).…”

Section: Hormone Analysesmentioning

confidence: 99%

Estrogen Replacement Therapy after the Menopause

Helgason

1982

Acta Obstet Gynecol Scand

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Dehydroepiandrosterone sulfate in plasma: hydrolysis, extraction and radioimmunoassay

Cited by 32 publications

References 8 publications

Serum androgens and sex hormone binding globulin in obese Pima Indian females

Serum androgens and sex hormone binding globulin in obese Pima Indian females

Treatment with oral estrone sulphate in the female climacteric: II. Hormonal aspects

Estrogen Replacement Therapy after the Menopause

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