Dehydroepiandrosterone Reduced Lipid Droplet Accumulation via Inhibiting Cell Proliferation and Improving Mitochondrial Function in Primary Chicken Hepatocytes

Abstract

Dehydroepiandrosterone (DHEA) possesses fat-reducing effect, while little information is available on whether DHEA regulates cell proliferation and mitochondrial function, which would, in turn, affect lipid droplet accumulation in the broiler. In the present study, the lipid droplet accumulation, cell proliferation, cell cycle and mitochondrial membrane potential were analysis in primary chicken hepatocytes after DHEA treated. The results showed that total area and counts of lipid droplets were significantly decreased in hepatocytes treated with DHEA. The cell viability was significantly increased, while cell proliferation was significantly inhibited in a dose-dependent manner in primary chicken hepatocytes after DHEA treated. DHEA treatment significantly increased the cell population in S phase and decreased the population in G2/M in primary chicken hepatocytes. Meanwhile, the cyclin A and cyclin-dependent kinases 2 (CDK2) mRNA abundance were significantly decreased in hepatocytes after DHEA treated. No significant differences were observed in the number of mitochondria, while the mitochondrial membrane permeability and succinate dehydrogenase (SDH) activity were significantly increased in hepatocytes after DHEA treated. In conclusion, our results demonstrated that DHEA reduced lipid droplet accumulation by inhibiting hepatocytes proliferation and enhancing mitochondrial function in primary chicken hepatocytes.

Osteocalcin reduces fat accumulation and inflammatory reaction by inhibiting ROS-JNK signal pathway in chicken embryonic hepatocytes

“…Primary chicken hepatocytes can be isolated from freshly perfused chicken liver, but the mixed cells from chicken liver also contain hepatic nonparenchymal cells including macrophages, stellate cells or biliary endothelial cells ( Mackei et al, 2020 ). It is easier to get purified hepatocytes using chicken embryonic livers ( Li et al, 2018 ; Wu et al, 2021a ). The purified hepatocytes can be identified by PAS staining which is used to specifically identify them by the markers of carbohydrates and stored glycogens ( Hui et al, 2017 ; Tao et al, 2021 ).…”

Beyond energy provider: multifunction of lipid droplets in embryonic development

“…The effect of adding a synthetic serum to cultured embryos was similar to that of adding fetal calf serum [ 61 ], and the serum did not cause lipid accumulation or organelle damage to bovine embryonic cells [ 62 ], reducing the concentration of fetal bovine serum to 5% for culturing in vitro embryonic cells is an appropriate concentration [ 63 ]. Supplementation with all-trans retinoic acid [ 64 ], L-carnitine [ 65 ], melatonin [ 66 ], and dehydroepiandrosterone [ 67 ] in serum can improve in vitro embryo culturing, which provides a method for the livestock industry including other fields that require embryo culturing in vitro, and hopefully, more serum additives will enhance embryo In vitro culture viability. However, human in vitro embryo culture is still in the IVF period, and there are many difficulties in the late in vitro culture technology.…”

PLIN2 Mediates Neuroinflammation and Oxidative/Nitrosative Stress via Downregulating Phosphatidylethanolamine in the Rostral Ventrolateral Medulla of Stressed Hypertensive Rats

“…[43][44][45] Inhibition of LDs accumulation regulates the metabolic pattern of proliferating cells and partially block cell proliferation signals. [46][47][48] Perilipins are the important structural protein of LDs membrane, which separates the hydrophobic lipid core from the cytoplasm and participates in the maintenance of LDs homeostasis. PLIN2 is an inherent perilipin protein, and its expression and phosphorylation are involved in lipolysis, lipophagy, lipid droplet fusion, and signal transduction.…”