Dehydrin expression in seeds: an issue of maturation drying

Kleinwächter, Maik; Radwan, Ahmed; Hara, Masakazu; Selmar, Dirk

doi:10.3389/fpls.2014.00402

Cited by 22 publications

(21 citation statements)

References 25 publications

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“…LEA proteins are associated mainly with water deficit tolerance, due to protein protection, membrane stabilisation, and ion sequestration (Battaglia and Covarrubias 2013). They are also involved in seed development, playing a protective role during maturation drying (Kleinwächter et al 2014). Hundertmark et al (2011) showed that seed-specific dehydrins play a role against deterioration during storage at low moisture content.…”

Section: Proteins Involved In Stress Responsesmentioning

confidence: 99%

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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& Key message Adequate storability of black poplar (Populus nigra L.) seeds at temperatures below 0°C was associated with preservation of proteins of energy and carbohydrate metabolism, protein turnover, and proteins maintaining longterm stability of dehydrated tissue. & Context Understanding seed storability is a key factor for effective seed preservation and conservation. Black poplar is an endangered tree species and its seed loses rapidly viability during storage. & Aims The aim of this study was to determine, and functionally characterise, the proteins associated with storability of black poplar seeds. & Methods Dried seeds (7.1% MC) were stored at 3°C, − 3°C, − 20°C, and − 196°C, for a period of 12 and 24 months. Proteins were extracted and separated according to their isoelectric point (pI) and mass using 2-dimensional electrophoresis. Proteins that varied in abundance for temperature and time of storage were identified by mass spectrometry. & Results A germination test showed that seeds remained viable at − 3, − 20, and − 196°C. Storage at 3°C caused loss of seed viability. This loss in seed vigour was related to the largest changes in protein abundance. As storage temperature decreased, a smaller number of proteins displayed changed abundance. & Conclusion Good storability of black poplar seeds under freezing conditions may be associated with the following: an inhibition of synthesis of energy and carbohydrate metabolism and protein turnover proteins, LEA proteins that maintain long-term stability of dehydrated tissue, GDSL esterases/lipases that inhibit hydrolysing activity, and by chaperonins that protect protein functionality. Storage conditions influence the preservation of protein function that governs seed viability.

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Section: Proteins Involved In Stress Responsesmentioning

confidence: 99%

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Pawłowski

Klupczyńska

Leśniewska

et al. 2019

Annals of Forest Science

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“…Another is a 6-phosphogluconate dehydrogenase protein, which are components of the pentose phosphate pathway and important for stress tolerance and seed growth (Spielbauer et al 2013;Hou et al 2007). Other MEGs are involved in desiccation and cold tolerance (Kleinwächter et al 2014;Borovskii et al 2002) and other stress tolerances (Liu et al 2015;Roy and Wu 2002), vacuolar storage (Bolte et al 2011;Jauh, Phillips, and Rogers 1999), metabolism and recycling, cell division and growth, RNA binding, and there is also a ribosomal protein.…”

Section: Putative Functions Of Imprinted Genesmentioning

confidence: 99%

Mechanisms driving endosperm-based hybrid incompatibilities: insights from hybrid monkeyflowers

et al. 2018

Preprint

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Angiosperm endosperm requires genomic and epigenomic interactions between maternal and paternal genomes for proper seed development. Genomic imprinting, an epigenetic phenomenon where the expression of certain genes is predominantly contributed by one parent, is an essential part of this process and unique to endosperm. Perturbation of imprinting can be fatal to developing seeds, and can be caused by interspecific or interploidy hybridization. However, underlying mechanisms driving these endosperm-based hybridization barriers are not well understood or described. Here we investigate the consequences of genomic imprinting in a naturally occurring interploidy and interspecies hybrid between the diploid, Mimulus guttatus, and the allotetraploid (with two subgenomes), M. luteus (Phrymaceae). We find that the two parental species differ in patterns of DNA methylation, gene expression, and imprinting. Hybrid crosses in both directions, which suffer from endosperm abnormalities and decreased germination rates, display altered methylation patterns compared to parent endosperm. Furthermore, imprinting and expression patterns appear perturbed in hybrid endosperm, where we observe global expression dominance of each of the two M. luteus subgenomes, which share similar expression patterns, over the M. guttatus genome, regardless of crossing direction. We suggest that epigenetic repatterning within the hybrid may drive global shifts in expression patterns and be the result of diverged epigenetic/regulatory landscapes between parental genomes. This may either establish or exacerbate dosage-based epistatic incompatibilities between the specific imprinting patterns that have diverged between parental species, thus driving potentially rapid endosperm-based hybridization barriers.

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“…In two papers published recently [48,49], the authors suggest an interesting point of view and "paradigm change": LEA proteins are synthesized as response to drought stress, which takes place at the end of maturation (of orthodox seeds). But it is known that dehydrins/LEA proteins are synthesized before maturation drying [6].…”

Section: Dehydrins In Orthodox and Recalcitrant Seedsmentioning

confidence: 99%

“…During embryogenesis and maturation drying of orthodox seeds, dehydrins are synthesized and seeds may survive dry storage. "Typical" recalcitrant seeds [48,49] such as Avicennia and Brunguiera have no dehydrins and high water content and they do not survive water loss during storage. Intermediate seeds (Coffea, Barringtinia) have no dehydrins during embryogenesis but have genes of dehydrins and may synthesize dehydrins after partial water loss in storage, so these seeds may survive [48,49].…”

Section: Dehydrins In Orthodox and Recalcitrant Seedsmentioning

confidence: 99%

“…"Typical" recalcitrant seeds [48,49] such as Avicennia and Brunguiera have no dehydrins and high water content and they do not survive water loss during storage. Intermediate seeds (Coffea, Barringtinia) have no dehydrins during embryogenesis but have genes of dehydrins and may synthesize dehydrins after partial water loss in storage, so these seeds may survive [48,49]. And "atypical" recalcitrant seeds (Camellia, Castanea, Euterpe, Quercus) accumulate some dehydrins at the end of embryogenesis and undergo a weak maturation drying and can be stored for a limited time [48,49].…”

Section: Dehydrins In Orthodox and Recalcitrant Seedsmentioning

confidence: 99%

See 1 more Smart Citation

Stress-Induced Proteins in Recalcitrant Seeds During Deep Dormancy and Early Germination

Azarkovich¹

2016

Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives

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The role and functions of dehydrins and heat shock proteins in seeds especially in desiccation-sensitive recalcitrant seeds are discussed.During the periods of dehydration, a wide variety of plants can express dehydration proteins dehydrins , which are also members of the plant late embryogenesis abundant LE" protein family. Dehydrins have been most extensively studied in relation to drought and cold stresses. Dehydrins are synthesized in orthodox seeds, and their development at the final stage is associated with genetically determined seeds drying. Dehydrins amount can reach % of total cell proteins. "t the same time, dehydrins are found in desiccation-sensitive recalcitrant seeds. The following are the functions of dehydrins with experimental evidence binding to water and ions, binding to phospholipids, radical scavenging, phosphorylation, binding to calcium, protection of enzymes, binding to cytoskeletons, and binding to nucleic acids.It seems evident that, in the embryo cells, heat shock induced changes in gene expression and HSP synthesis but did not result in translational discrimination of mRN"s for nonheat shock proteins. Such specific feature has been observed earlier for orthodox seeds during their development and early stages of germination. It is suggested that such response to HS is characteristic just of embryo tissues it could be considered an additional molecular mechanism improving embryo tolerance to unfavorable environmental conditions.

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Dehydrin expression in seeds: an issue of maturation drying

Cited by 22 publications

References 25 publications

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Proteomic analysis of black poplar (Populus nigra L.) seed storability

Mechanisms driving endosperm-based hybrid incompatibilities: insights from hybrid monkeyflowers

Stress-Induced Proteins in Recalcitrant Seeds During Deep Dormancy and Early Germination

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